Modified Black Soldier Fly Larva Fat in Broiler Diet: Effects on Performance, Carcass Traits, Blood Parameters, Histomorphological Features and Gut Microbiota

SIMPLE SUMMARY: Black soldier fly (Hermetia illucens L.; BSF) is gaining interest as a functional feed additive, due to the high amount of medium-chain fatty acids (MCFAs) and monoglycerides, which provide antimicrobial activities and stimulate gastrointestinal health through inhibition of potentially pathogenic bacteria. The present study evaluated the effect of BSF and modified BSF larvae fat in broiler chicken’s diet. Overall results were comparable among the studied diets, suggesting that modified BSF larvae fat showed a positive modulation of fecal microbiota by a positive reduction in potentially pathogenic bacteria such as Clostridium and Corynebacterium, without affecting intestinal morphology or showing any adverse histopathological alternations. ABSTRACT: In this study, a total of 200 male broiler chickens (Ross 308) were assigned to four dietary treatments (5 pens/treatment and 10 birds/pen) for two feeding phases: starter (0–11 days of age) and grower-finisher (11–33 days of age). A basal diet containing soy oil (SO) as added fat was used as control group (C), tested against three experimental diets where the SO was partially substituted by BSF larvae fat (BSF) or one of two types of modified BSF larvae fat (MBSF1 and MBSF2, respectively). The two modified BSF larvae fats had a high and low ratio of monobutyrin to monoglycerides of medium chain fatty acid, respectively. Diet did not influence the growth or slaughter performance, pH, color, or the chemical composition of breast and thigh muscles, gut morphometric indices, or histopathological alterations in all the organs. As far as fecal microbiota are concerned, MBSF1 and MBSF2 diets reduced the presence of Clostridium and Corynebacterium, which can frequently cause infection in poultry. In conclusion, modified BSF larva fat may positively modulate the fecal microbiota of broiler chickens without influencing the growth performance and intestinal morphology or showing any adverse histopathological alternations.