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First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

Bovine respiratory disease (BRD) causes high morbidity and mortality in beef cattle worldwide. Antimicrobial resistance (AMR) monitoring of BRD pathogens is critical to promote appropriate antimicrobial stewardship in veterinary medicine for optimal treatment and control. Here, the susceptibility of...

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Autores principales: Alhamami, Tamara, Chowdhury, Piklu Roy, Gomes, Nancy, Carr, Mandi, Veltman, Tania, Khazandi, Manouchehr, Mollinger, Joanne, Deutscher, Ania T., Turni, Conny, Mahdi, Layla, Venter, Henrietta, Abraham, Sam, Djordjevic, Steven P., Trott, Darren J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233904/
https://www.ncbi.nlm.nih.gov/pubmed/34204544
http://dx.doi.org/10.3390/microorganisms9061322
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author Alhamami, Tamara
Chowdhury, Piklu Roy
Gomes, Nancy
Carr, Mandi
Veltman, Tania
Khazandi, Manouchehr
Mollinger, Joanne
Deutscher, Ania T.
Turni, Conny
Mahdi, Layla
Venter, Henrietta
Abraham, Sam
Djordjevic, Steven P.
Trott, Darren J.
author_facet Alhamami, Tamara
Chowdhury, Piklu Roy
Gomes, Nancy
Carr, Mandi
Veltman, Tania
Khazandi, Manouchehr
Mollinger, Joanne
Deutscher, Ania T.
Turni, Conny
Mahdi, Layla
Venter, Henrietta
Abraham, Sam
Djordjevic, Steven P.
Trott, Darren J.
author_sort Alhamami, Tamara
collection PubMed
description Bovine respiratory disease (BRD) causes high morbidity and mortality in beef cattle worldwide. Antimicrobial resistance (AMR) monitoring of BRD pathogens is critical to promote appropriate antimicrobial stewardship in veterinary medicine for optimal treatment and control. Here, the susceptibility of Mannheimia haemolytica and Pasteurella multicoda isolates obtained from BRD clinical cases (deep lung swabs at post-mortem) among feedlots in four Australian states (2014–2019) was determined for 19 antimicrobial agents. The M. haemolytica isolates were pan-susceptible to all tested agents apart from a single macrolide-resistant isolate (1/88; 1.1%) from New South Wales (NSW). Much higher frequencies of P. multocida isolates were resistant to tetracycline (18/140; 12.9%), tilmicosin (19/140; 13.6%), tulathromycin/gamithromycin (17/140; 12.1%), and ampicillin/penicillin (6/140; 4.6%). Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. Random-amplified polymorphic DNA (RAPD) typing identified a high degree of genetic homogeneity among the M. haemolytica isolates, whereas P. multocida isolates were more heterogeneous. Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and bla(ROB-1) encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. The exception was the tilmicosin-resistant, tulathromycin/gamithromycin-susceptible phenotype identified in two Queensland isolates, the genetic basis of which could not be determined. These results confirm the first emergence of AMR in M. haemolytica and P. multocida from BRD cases in Australia, which should be closely monitored.
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spelling pubmed-82339042021-06-27 First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia Alhamami, Tamara Chowdhury, Piklu Roy Gomes, Nancy Carr, Mandi Veltman, Tania Khazandi, Manouchehr Mollinger, Joanne Deutscher, Ania T. Turni, Conny Mahdi, Layla Venter, Henrietta Abraham, Sam Djordjevic, Steven P. Trott, Darren J. Microorganisms Article Bovine respiratory disease (BRD) causes high morbidity and mortality in beef cattle worldwide. Antimicrobial resistance (AMR) monitoring of BRD pathogens is critical to promote appropriate antimicrobial stewardship in veterinary medicine for optimal treatment and control. Here, the susceptibility of Mannheimia haemolytica and Pasteurella multicoda isolates obtained from BRD clinical cases (deep lung swabs at post-mortem) among feedlots in four Australian states (2014–2019) was determined for 19 antimicrobial agents. The M. haemolytica isolates were pan-susceptible to all tested agents apart from a single macrolide-resistant isolate (1/88; 1.1%) from New South Wales (NSW). Much higher frequencies of P. multocida isolates were resistant to tetracycline (18/140; 12.9%), tilmicosin (19/140; 13.6%), tulathromycin/gamithromycin (17/140; 12.1%), and ampicillin/penicillin (6/140; 4.6%). Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. Random-amplified polymorphic DNA (RAPD) typing identified a high degree of genetic homogeneity among the M. haemolytica isolates, whereas P. multocida isolates were more heterogeneous. Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and bla(ROB-1) encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. The exception was the tilmicosin-resistant, tulathromycin/gamithromycin-susceptible phenotype identified in two Queensland isolates, the genetic basis of which could not be determined. These results confirm the first emergence of AMR in M. haemolytica and P. multocida from BRD cases in Australia, which should be closely monitored. MDPI 2021-06-17 /pmc/articles/PMC8233904/ /pubmed/34204544 http://dx.doi.org/10.3390/microorganisms9061322 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alhamami, Tamara
Chowdhury, Piklu Roy
Gomes, Nancy
Carr, Mandi
Veltman, Tania
Khazandi, Manouchehr
Mollinger, Joanne
Deutscher, Ania T.
Turni, Conny
Mahdi, Layla
Venter, Henrietta
Abraham, Sam
Djordjevic, Steven P.
Trott, Darren J.
First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
title First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
title_full First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
title_fullStr First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
title_full_unstemmed First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
title_short First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
title_sort first emergence of resistance to macrolides and tetracycline identified in mannheimia haemolytica and pasteurella multocida isolates from beef feedlots in australia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233904/
https://www.ncbi.nlm.nih.gov/pubmed/34204544
http://dx.doi.org/10.3390/microorganisms9061322
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