Analysis of the Gastro-Intestinal Tract of Marine Mammals: A Multidisciplinary Approach with a New Multi-Sieves Tool

SIMPLE SUMMARY: Currently procedures used to obtain samples from the gastro-intestinal tract (GIT) and protocols used to perform their respective analyses do not allow a multidisciplinary approach of this system. In fact, the investigations applied on the GIT, when performed singularly, could impair or limit the other analyses, because the currently procedures do not consider the needs of other disciplines. This means that the analyses to perform must be selected a priori, sacrificing the collection of other types of data and leads to the risk of losing important information, especially for wildlife species. To solve this conflict, we implement and standardize a new methodological approach to the GIT of marine mammals, which allow the collection of samples for different disciplines at the same time, performing the respective analyses, interpret and compare their results in a multidisciplinary way. The compatibility of multiple analyses allows the gaining of more information about the cause of death of stranded marine mammals and to enhance the knowledge of their biology and ecology. ABSTRACT: Organs and content of the gastro-intestinal tract (GIT) of marine mammals are relevant for a variety of investigations and provide data to researchers from different fields. Currently used protocols applied to the GIT for specific analysis limit the possibility to execute other investigations and important information could be lost. To ensure a proper sample collection and a multidisciplinary investigation of the GIT of marine mammals, a new multi-sieves tool and a specific protocol have been developed. This new device and approach allowed the simultaneous sampling of the GIT and its content for the main investigations concerned. The samples collected during these preliminary trials were suitable to perform all the different research procedures considered in this work. The obtained results show that with a few and easy procedural adjustments, a multidisciplinary sampling and evaluation of the GIT of marine mammals is possible. This will reduce the risk of losing important data aimed at understanding the cause of death of the animal, but also biology and ecology of marine mammals, and other important data for their conservation and habitats management.