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Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate
In recent years, there has been increasing interest in the treatment of bone defects using undifferentiated mesenchymal stem cells (MSCs) in vivo. Recently, dental pulp has been proposed as a promising source of pluripotent mesenchymal stem cells (MSCs), which can be used in various clinical applica...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234467/ https://www.ncbi.nlm.nih.gov/pubmed/34202970 http://dx.doi.org/10.3390/ma14123409 |
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author | Takabatake, Kiyofumi Nakano, Keisuke Kawai, Hotaka Inada, Yasunori Sukegawa, Shintaro Qiusheng, Shan Fushimi, Shigeko Tsujigiwa, Hidetsugu Nagatsuka, Hitoshi |
author_facet | Takabatake, Kiyofumi Nakano, Keisuke Kawai, Hotaka Inada, Yasunori Sukegawa, Shintaro Qiusheng, Shan Fushimi, Shigeko Tsujigiwa, Hidetsugu Nagatsuka, Hitoshi |
author_sort | Takabatake, Kiyofumi |
collection | PubMed |
description | In recent years, there has been increasing interest in the treatment of bone defects using undifferentiated mesenchymal stem cells (MSCs) in vivo. Recently, dental pulp has been proposed as a promising source of pluripotent mesenchymal stem cells (MSCs), which can be used in various clinical applications. Dentin is the hard tissue that makes up teeth, and has the same composition and strength as bone. However, unlike bone, dentin is usually not remodeled under physiological conditions. Here, we generated odontoblast-like cells from mouse dental pulp stem cells and combined them with honeycomb tricalcium phosphate (TCP) with a 300 μm hole to create bone-like tissue under the skin of mice. The bone-like hard tissue produced in this study was different from bone tissue, i.e., was not resorbed by osteoclasts and was less easily absorbed than the bone tissue. It has been suggested that hard tissue-forming cells induced from dental pulp do not have the ability to induce osteoclast differentiation. Therefore, the newly created bone-like hard tissue has high potential for absorption-resistant hard tissue repair and regeneration procedures. |
format | Online Article Text |
id | pubmed-8234467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82344672021-06-27 Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate Takabatake, Kiyofumi Nakano, Keisuke Kawai, Hotaka Inada, Yasunori Sukegawa, Shintaro Qiusheng, Shan Fushimi, Shigeko Tsujigiwa, Hidetsugu Nagatsuka, Hitoshi Materials (Basel) Article In recent years, there has been increasing interest in the treatment of bone defects using undifferentiated mesenchymal stem cells (MSCs) in vivo. Recently, dental pulp has been proposed as a promising source of pluripotent mesenchymal stem cells (MSCs), which can be used in various clinical applications. Dentin is the hard tissue that makes up teeth, and has the same composition and strength as bone. However, unlike bone, dentin is usually not remodeled under physiological conditions. Here, we generated odontoblast-like cells from mouse dental pulp stem cells and combined them with honeycomb tricalcium phosphate (TCP) with a 300 μm hole to create bone-like tissue under the skin of mice. The bone-like hard tissue produced in this study was different from bone tissue, i.e., was not resorbed by osteoclasts and was less easily absorbed than the bone tissue. It has been suggested that hard tissue-forming cells induced from dental pulp do not have the ability to induce osteoclast differentiation. Therefore, the newly created bone-like hard tissue has high potential for absorption-resistant hard tissue repair and regeneration procedures. MDPI 2021-06-20 /pmc/articles/PMC8234467/ /pubmed/34202970 http://dx.doi.org/10.3390/ma14123409 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Takabatake, Kiyofumi Nakano, Keisuke Kawai, Hotaka Inada, Yasunori Sukegawa, Shintaro Qiusheng, Shan Fushimi, Shigeko Tsujigiwa, Hidetsugu Nagatsuka, Hitoshi Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate |
title | Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate |
title_full | Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate |
title_fullStr | Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate |
title_full_unstemmed | Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate |
title_short | Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate |
title_sort | preparation of absorption-resistant hard tissue using dental pulp-derived cells and honeycomb tricalcium phosphate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234467/ https://www.ncbi.nlm.nih.gov/pubmed/34202970 http://dx.doi.org/10.3390/ma14123409 |
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