Serological Survey on the Occurrence of Rickettsia spp., Neospora caninum, Bartonella henselae and Toxoplasma gondii in Cats from Tuscany (Central Italy)

SIMPLE SUMMARY: Domestic and stray cats are frequently infected by pathogens, some of which are zoonotic. The occurrence of microorganisms such as Bartonella henselae and Toxoplasma gondii, even though they are well known, could be underestimated because they are not regularly investigated in cats. Other pathogens, such as Neospora caninum and Rickettsia spp., have not been largely studied in feline populations and data about their spreading in Italian cats are very scanty. Monitoring of domestic and stray cats for these pathogens is important to evaluate the health status of the animals, but also, regarding B. henselae, Rickettsia spp. and T. gondii, from a One Health perspective. ABSTRACT: Asymptomatic cats often harbor pathogens, some of which have not been largely investigated in feline populations. The aim of this study was to evaluate the occurrence of antibodies against Rickettsia conorii, Rickettsia felis, Rickettsia typhi, Neospora caninum, Bartonella henselae and Toxoplasma gondii in cats from Tuscany. Ninety-five blood serum samples, previously collected, were analyzed by indirect immunofluorescence assay. Fifty-six (58.94%) cats had antibodies to at least one investigated pathogen: 28 (29.47%) cats were positive for B. henselae, 17 (17.89%) for R. felis, 14 (14.73%) for R. conorii, 14 (14.73%) for T. gondii, 2 (2.1%) for N. caninum. No cats were positive for R. typhi. Positive reactions to two or more pathogens were detected in 18 (18.94%) cats. The occurrence of antibodies against these microorganisms suggests that cats, even though asymptomatic, may be infected by pathogens, often zoonotic, and thus may be a source of infections for other animals and humans.