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Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host

Facultative intracellular symbionts like the α-proteobacteria Wolbachia influence their insect host phenotype but little is known about how much they affect their host microbiota. Here, we quantified the impact of Wolbachia infection on the bacterial community of the cabbage root fly Delia radicum b...

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Autores principales: Ourry, Morgane, Crosland, Agathe, Lopez, Valérie, Derocles, Stéphane A. P., Mougel, Christophe, Cortesero, Anne-Marie, Poinsot, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234596/
https://www.ncbi.nlm.nih.gov/pubmed/34208681
http://dx.doi.org/10.3390/microorganisms9061313
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author Ourry, Morgane
Crosland, Agathe
Lopez, Valérie
Derocles, Stéphane A. P.
Mougel, Christophe
Cortesero, Anne-Marie
Poinsot, Denis
author_facet Ourry, Morgane
Crosland, Agathe
Lopez, Valérie
Derocles, Stéphane A. P.
Mougel, Christophe
Cortesero, Anne-Marie
Poinsot, Denis
author_sort Ourry, Morgane
collection PubMed
description Facultative intracellular symbionts like the α-proteobacteria Wolbachia influence their insect host phenotype but little is known about how much they affect their host microbiota. Here, we quantified the impact of Wolbachia infection on the bacterial community of the cabbage root fly Delia radicum by comparing the microbiota of Wolbachia-free and infected adult flies of both sexes. We used high-throughput DNA sequencing (Illumina MiSeq, 16S rRNA, V5-V7 region) and performed a community and a network analysis. In both sexes, Wolbachia infection significantly decreased the diversity of D. radicum bacterial communities and modified their structure and composition by reducing abundance in some taxa but increasing it in others. Infection by Wolbachia was negatively correlated to 8 bacteria genera (Erwinia was the most impacted), and positively correlated to Providencia and Serratia. We suggest that Wolbachia might antagonize Erwinia for being entomopathogenic (and potentially intracellular), but would favor Providencia and Serratia because they might protect the host against chemical plant defenses. Although they might seem prisoners in a cell, endocellular symbionts can impact the whole microbiota of their host, hence its extended phenotype, which provides them with a way to interact with the outside world.
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spelling pubmed-82345962021-06-27 Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host Ourry, Morgane Crosland, Agathe Lopez, Valérie Derocles, Stéphane A. P. Mougel, Christophe Cortesero, Anne-Marie Poinsot, Denis Microorganisms Article Facultative intracellular symbionts like the α-proteobacteria Wolbachia influence their insect host phenotype but little is known about how much they affect their host microbiota. Here, we quantified the impact of Wolbachia infection on the bacterial community of the cabbage root fly Delia radicum by comparing the microbiota of Wolbachia-free and infected adult flies of both sexes. We used high-throughput DNA sequencing (Illumina MiSeq, 16S rRNA, V5-V7 region) and performed a community and a network analysis. In both sexes, Wolbachia infection significantly decreased the diversity of D. radicum bacterial communities and modified their structure and composition by reducing abundance in some taxa but increasing it in others. Infection by Wolbachia was negatively correlated to 8 bacteria genera (Erwinia was the most impacted), and positively correlated to Providencia and Serratia. We suggest that Wolbachia might antagonize Erwinia for being entomopathogenic (and potentially intracellular), but would favor Providencia and Serratia because they might protect the host against chemical plant defenses. Although they might seem prisoners in a cell, endocellular symbionts can impact the whole microbiota of their host, hence its extended phenotype, which provides them with a way to interact with the outside world. MDPI 2021-06-16 /pmc/articles/PMC8234596/ /pubmed/34208681 http://dx.doi.org/10.3390/microorganisms9061313 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ourry, Morgane
Crosland, Agathe
Lopez, Valérie
Derocles, Stéphane A. P.
Mougel, Christophe
Cortesero, Anne-Marie
Poinsot, Denis
Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host
title Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host
title_full Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host
title_fullStr Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host
title_full_unstemmed Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host
title_short Influential Insider: Wolbachia, an Intracellular Symbiont, Manipulates Bacterial Diversity in Its Insect Host
title_sort influential insider: wolbachia, an intracellular symbiont, manipulates bacterial diversity in its insect host
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234596/
https://www.ncbi.nlm.nih.gov/pubmed/34208681
http://dx.doi.org/10.3390/microorganisms9061313
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