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The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae

Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417...

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Autores principales: Raya Tonetti, Fernanda, Tomokiyo, Mikado, Ortiz Moyano, Ramiro, Quilodrán-Vega, Sandra, Yamamuro, Hikari, Kanmani, Paulraj, Melnikov, Vyacheslav, Kurata, Shoichiro, Kitazawa, Haruki, Villena, Julio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234606/
https://www.ncbi.nlm.nih.gov/pubmed/34207076
http://dx.doi.org/10.3390/microorganisms9061324
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author Raya Tonetti, Fernanda
Tomokiyo, Mikado
Ortiz Moyano, Ramiro
Quilodrán-Vega, Sandra
Yamamuro, Hikari
Kanmani, Paulraj
Melnikov, Vyacheslav
Kurata, Shoichiro
Kitazawa, Haruki
Villena, Julio
author_facet Raya Tonetti, Fernanda
Tomokiyo, Mikado
Ortiz Moyano, Ramiro
Quilodrán-Vega, Sandra
Yamamuro, Hikari
Kanmani, Paulraj
Melnikov, Vyacheslav
Kurata, Shoichiro
Kitazawa, Haruki
Villena, Julio
author_sort Raya Tonetti, Fernanda
collection PubMed
description Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.
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spelling pubmed-82346062021-06-27 The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae Raya Tonetti, Fernanda Tomokiyo, Mikado Ortiz Moyano, Ramiro Quilodrán-Vega, Sandra Yamamuro, Hikari Kanmani, Paulraj Melnikov, Vyacheslav Kurata, Shoichiro Kitazawa, Haruki Villena, Julio Microorganisms Article Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics. MDPI 2021-06-18 /pmc/articles/PMC8234606/ /pubmed/34207076 http://dx.doi.org/10.3390/microorganisms9061324 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raya Tonetti, Fernanda
Tomokiyo, Mikado
Ortiz Moyano, Ramiro
Quilodrán-Vega, Sandra
Yamamuro, Hikari
Kanmani, Paulraj
Melnikov, Vyacheslav
Kurata, Shoichiro
Kitazawa, Haruki
Villena, Julio
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_full The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_fullStr The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_full_unstemmed The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_short The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
title_sort respiratory commensal bacterium dolosigranulum pigrum 040417 improves the innate immune response to streptococcus pneumoniae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234606/
https://www.ncbi.nlm.nih.gov/pubmed/34207076
http://dx.doi.org/10.3390/microorganisms9061324
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