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Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion

Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present stu...

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Autores principales: Ogawa, Mizuki, Udono, Miyako, Teruya, Kiichiro, Uehara, Norihisa, Katakura, Yoshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234638/
https://www.ncbi.nlm.nih.gov/pubmed/34207142
http://dx.doi.org/10.3390/nu13062087
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author Ogawa, Mizuki
Udono, Miyako
Teruya, Kiichiro
Uehara, Norihisa
Katakura, Yoshinori
author_facet Ogawa, Mizuki
Udono, Miyako
Teruya, Kiichiro
Uehara, Norihisa
Katakura, Yoshinori
author_sort Ogawa, Mizuki
collection PubMed
description Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth.
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spelling pubmed-82346382021-06-27 Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion Ogawa, Mizuki Udono, Miyako Teruya, Kiichiro Uehara, Norihisa Katakura, Yoshinori Nutrients Article Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth. MDPI 2021-06-18 /pmc/articles/PMC8234638/ /pubmed/34207142 http://dx.doi.org/10.3390/nu13062087 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ogawa, Mizuki
Udono, Miyako
Teruya, Kiichiro
Uehara, Norihisa
Katakura, Yoshinori
Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
title Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
title_full Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
title_fullStr Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
title_full_unstemmed Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
title_short Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
title_sort exosomes derived from fisetin-treated keratinocytes mediate hair growth promotion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234638/
https://www.ncbi.nlm.nih.gov/pubmed/34207142
http://dx.doi.org/10.3390/nu13062087
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