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Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present stu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234638/ https://www.ncbi.nlm.nih.gov/pubmed/34207142 http://dx.doi.org/10.3390/nu13062087 |
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author | Ogawa, Mizuki Udono, Miyako Teruya, Kiichiro Uehara, Norihisa Katakura, Yoshinori |
author_facet | Ogawa, Mizuki Udono, Miyako Teruya, Kiichiro Uehara, Norihisa Katakura, Yoshinori |
author_sort | Ogawa, Mizuki |
collection | PubMed |
description | Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth. |
format | Online Article Text |
id | pubmed-8234638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82346382021-06-27 Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion Ogawa, Mizuki Udono, Miyako Teruya, Kiichiro Uehara, Norihisa Katakura, Yoshinori Nutrients Article Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth. MDPI 2021-06-18 /pmc/articles/PMC8234638/ /pubmed/34207142 http://dx.doi.org/10.3390/nu13062087 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ogawa, Mizuki Udono, Miyako Teruya, Kiichiro Uehara, Norihisa Katakura, Yoshinori Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
title | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
title_full | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
title_fullStr | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
title_full_unstemmed | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
title_short | Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion |
title_sort | exosomes derived from fisetin-treated keratinocytes mediate hair growth promotion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234638/ https://www.ncbi.nlm.nih.gov/pubmed/34207142 http://dx.doi.org/10.3390/nu13062087 |
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