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Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers

Haptoglobin (Hp) is a blood plasma glycoprotein that plays a critical role in tissue protection and the prevention of oxidative damage. Haptoglobin is an acute-phase protein, its concentration in plasma changes in pathology, and the test for its concentration is part of normal clinical practice. Hap...

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Autores principales: Naryzhny, Stanislav, Ronzhina, Natalia, Zorina, Elena, Kabachenko, Fedor, Zavialova, Maria, Zgoda, Viktor, Klopov, Nikolai, Legina, Olga, Pantina, Rimma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234662/
https://www.ncbi.nlm.nih.gov/pubmed/34207114
http://dx.doi.org/10.3390/ijms22126533
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author Naryzhny, Stanislav
Ronzhina, Natalia
Zorina, Elena
Kabachenko, Fedor
Zavialova, Maria
Zgoda, Viktor
Klopov, Nikolai
Legina, Olga
Pantina, Rimma
author_facet Naryzhny, Stanislav
Ronzhina, Natalia
Zorina, Elena
Kabachenko, Fedor
Zavialova, Maria
Zgoda, Viktor
Klopov, Nikolai
Legina, Olga
Pantina, Rimma
author_sort Naryzhny, Stanislav
collection PubMed
description Haptoglobin (Hp) is a blood plasma glycoprotein that plays a critical role in tissue protection and the prevention of oxidative damage. Haptoglobin is an acute-phase protein, its concentration in plasma changes in pathology, and the test for its concentration is part of normal clinical practice. Haptoglobin is a conservative protein and is the subject of research as a potential biomarker of many diseases, including malignant neoplasms. The Human Hp gene is polymorphic and controls the synthesis of three major phenotypes—homozygous Hp1-1 and Hp2-2, and heterozygous Hp2-1, determined by a combination of allelic variants that are inherited. Numerous studies indicate that the phenotype of haptoglobin can be used to judge the individual’s predisposition to various diseases. In addition, Hp undergoes various post-translational modifications (PTMs). Glioblastoma multiform (GBM) is the most malignant primary brain tumor. In our study, we have analyzed the state of Hp proteoforms in plasma and cells using 1D (SDS-PAGE) and 2D electrophoresis (2DE) with the following mass spectrometry (LC ES-MS/MS) or Western blotting. We found that the levels of α2- and β-chain proteoforms are up-regulated in the plasma of GBM patients. An unprocessed form of Hp2-2 (PreHp2-2, zonulin) with unusual biophysical parameters (pI/Mw) was also detected in the plasma of GBM patients and glioblastoma cells. Altogether, this data shows the possibility to use proteoforms of haptoglobin as a potential GBM-specific plasma biomarker.
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spelling pubmed-82346622021-06-27 Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers Naryzhny, Stanislav Ronzhina, Natalia Zorina, Elena Kabachenko, Fedor Zavialova, Maria Zgoda, Viktor Klopov, Nikolai Legina, Olga Pantina, Rimma Int J Mol Sci Article Haptoglobin (Hp) is a blood plasma glycoprotein that plays a critical role in tissue protection and the prevention of oxidative damage. Haptoglobin is an acute-phase protein, its concentration in plasma changes in pathology, and the test for its concentration is part of normal clinical practice. Haptoglobin is a conservative protein and is the subject of research as a potential biomarker of many diseases, including malignant neoplasms. The Human Hp gene is polymorphic and controls the synthesis of three major phenotypes—homozygous Hp1-1 and Hp2-2, and heterozygous Hp2-1, determined by a combination of allelic variants that are inherited. Numerous studies indicate that the phenotype of haptoglobin can be used to judge the individual’s predisposition to various diseases. In addition, Hp undergoes various post-translational modifications (PTMs). Glioblastoma multiform (GBM) is the most malignant primary brain tumor. In our study, we have analyzed the state of Hp proteoforms in plasma and cells using 1D (SDS-PAGE) and 2D electrophoresis (2DE) with the following mass spectrometry (LC ES-MS/MS) or Western blotting. We found that the levels of α2- and β-chain proteoforms are up-regulated in the plasma of GBM patients. An unprocessed form of Hp2-2 (PreHp2-2, zonulin) with unusual biophysical parameters (pI/Mw) was also detected in the plasma of GBM patients and glioblastoma cells. Altogether, this data shows the possibility to use proteoforms of haptoglobin as a potential GBM-specific plasma biomarker. MDPI 2021-06-18 /pmc/articles/PMC8234662/ /pubmed/34207114 http://dx.doi.org/10.3390/ijms22126533 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naryzhny, Stanislav
Ronzhina, Natalia
Zorina, Elena
Kabachenko, Fedor
Zavialova, Maria
Zgoda, Viktor
Klopov, Nikolai
Legina, Olga
Pantina, Rimma
Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers
title Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers
title_full Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers
title_fullStr Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers
title_full_unstemmed Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers
title_short Evaluation of Haptoglobin and Its Proteoforms as Glioblastoma Markers
title_sort evaluation of haptoglobin and its proteoforms as glioblastoma markers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234662/
https://www.ncbi.nlm.nih.gov/pubmed/34207114
http://dx.doi.org/10.3390/ijms22126533
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