OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice

Otogelin-like protein (encoded by Otogl) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructive azoosp...

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Autores principales: Li, Zhiming, Zhang, Yan, Zhang, Xinzong, Cao, Congcong, Luo, Xiaomin, Gui, Yaoting, Tang, Yunge, Yuan, Shuiqiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234668/
https://www.ncbi.nlm.nih.gov/pubmed/34174893
http://dx.doi.org/10.1186/s12958-021-00779-0
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author Li, Zhiming
Zhang, Yan
Zhang, Xinzong
Cao, Congcong
Luo, Xiaomin
Gui, Yaoting
Tang, Yunge
Yuan, Shuiqiao
author_facet Li, Zhiming
Zhang, Yan
Zhang, Xinzong
Cao, Congcong
Luo, Xiaomin
Gui, Yaoting
Tang, Yunge
Yuan, Shuiqiao
author_sort Li, Zhiming
collection PubMed
description Otogelin-like protein (encoded by Otogl) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructive azoospermia (NOA), OTOGL displays the high mutant ratio (13.99 %). Then, we examined the expression of OTOGL in developing mouse testes. Otogl mRNA and protein are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to P21 testes exhibiting a decreased trend with the age growth. We thus generated a global Otogl knockout mouse (KO) model using the CRISPR/Cas9 technology; however, Otogl KO mice displayed normal development and fertility. Further histological analysis of Otogl knockout mouse testes revealed that all types of spermatogenic cells are present in Otogl KO seminiferous tubules. Together, our study suggested that OTOGL is nonessential for male germ cell development and spermatogenesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-021-00779-0.
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spelling pubmed-82346682021-06-28 OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice Li, Zhiming Zhang, Yan Zhang, Xinzong Cao, Congcong Luo, Xiaomin Gui, Yaoting Tang, Yunge Yuan, Shuiqiao Reprod Biol Endocrinol Research Otogelin-like protein (encoded by Otogl) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructive azoospermia (NOA), OTOGL displays the high mutant ratio (13.99 %). Then, we examined the expression of OTOGL in developing mouse testes. Otogl mRNA and protein are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to P21 testes exhibiting a decreased trend with the age growth. We thus generated a global Otogl knockout mouse (KO) model using the CRISPR/Cas9 technology; however, Otogl KO mice displayed normal development and fertility. Further histological analysis of Otogl knockout mouse testes revealed that all types of spermatogenic cells are present in Otogl KO seminiferous tubules. Together, our study suggested that OTOGL is nonessential for male germ cell development and spermatogenesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-021-00779-0. BioMed Central 2021-06-26 /pmc/articles/PMC8234668/ /pubmed/34174893 http://dx.doi.org/10.1186/s12958-021-00779-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Zhiming
Zhang, Yan
Zhang, Xinzong
Cao, Congcong
Luo, Xiaomin
Gui, Yaoting
Tang, Yunge
Yuan, Shuiqiao
OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
title OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
title_full OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
title_fullStr OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
title_full_unstemmed OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
title_short OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
title_sort otogl, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234668/
https://www.ncbi.nlm.nih.gov/pubmed/34174893
http://dx.doi.org/10.1186/s12958-021-00779-0
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