Increased Longevity and Dormancy of Soil-Buried Seeds from Advanced Crop–Wild Rice Hybrids Overexpressing the EPSPS Transgene

SIMPLE SUMMARY: Estimating the survival and reproductive ability caused by a transgene moved from a genetically engineered (GE) crop to its wild relative populations through gene flow plays an important role in assessing the potential environmental risks of the GE crop. Such estimation has essentially focused on the survival and reproduction-related characteristics above the ground, but with little attention to the GE seeds shattered in the soil seed banks. We demonstrated that the herbicide-resistant transgene overexpressing the rice endogenous EPSP enzyme increased the survival and longevity of the GE crop–wild (Oryza rufipogon) hybrid seeds in soil seed banks. In addition, enhanced survival and longevity of the GE hybrid seeds are likely associated with increases in seed dormancy and a growth hormone (auxin) via overexpressing the EPSPS transgene. Therefore, the EPSPS transgene can persist in the soil seed banks and spread in the environment, causing unwanted environmental impacts. ABSTRACT: Estimating the fitness effect conferred by a transgene introgressed into populations of wild relative species from a genetically engineered (GE) crop plays an important role in assessing the potential environmental risks caused by transgene flow. Such estimation has essentially focused on the survival and fecundity-related characteristics measured above the ground, but with little attention to the fate of GE seeds shattered in the soil seed banks after maturation. To explore the survival and longevity of GE seeds in soil, we examined the germination behaviors of crop–wild hybrid seeds (F(4)–F(6)) from the lineages of a GE herbicide-tolerant rice (Oryza sativa) line that contains an endogenous EPSPS transgene hybridized with two wild O. rufipogon populations after the seeds were buried in soil. The results showed significantly increased germination of the GE crop–wild hybrid seeds after soil burial, compared with that of the non-GE hybrid seeds. Additionally, the proportion of dormant seeds and the content of the growth hormone auxin (indole-3-acetic acid, IAA) in the GE crop–wild hybrid seeds significantly increased. Evidently, the EPSPS transgene enhances the survival and longevity of GE crop–wild rice seeds in the soil seed banks. The enhanced survival and longevity of the GE hybrid seeds is likely associated with the increases in seed dormancy and auxin (IAA) by overexpressing the rice endogenous EPSPS transgene. Thus, the fate of GE seeds in the soil seed banks should be earnestly considered when assessing the environmental risks caused by transgene flow.