Cargando…

The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema

The ostrich oil of Struthio camelus (Ratite) found uses in folk medicine as an anti-inflammatory in eczema and contact dermatitis. The anti-inflammatory effect of a γ-lactone (5-hexyl-3H-furan-2-one) isolated from ostrich oil and its formulated nano-emulsion in formalin-induced paw edema was investi...

Descripción completa

Detalles Bibliográficos
Autores principales: Eltom, Salah E. M., Abdellatif, Ahmed A. H., Maswadeh, Hamzah, Al-Omar, Mohsen S., Abdel-Hafez, Atef A., Mohammed, Hamdoon A., Agabein, Eiman ME., Alqasoomi, Ibrahim, Alrashidi, Salem A., Sajid, Mohammed S. M., Mobark, Mugahid A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234909/
https://www.ncbi.nlm.nih.gov/pubmed/34204472
http://dx.doi.org/10.3390/molecules26123701
_version_ 1783714192396648448
author Eltom, Salah E. M.
Abdellatif, Ahmed A. H.
Maswadeh, Hamzah
Al-Omar, Mohsen S.
Abdel-Hafez, Atef A.
Mohammed, Hamdoon A.
Agabein, Eiman ME.
Alqasoomi, Ibrahim
Alrashidi, Salem A.
Sajid, Mohammed S. M.
Mobark, Mugahid A.
author_facet Eltom, Salah E. M.
Abdellatif, Ahmed A. H.
Maswadeh, Hamzah
Al-Omar, Mohsen S.
Abdel-Hafez, Atef A.
Mohammed, Hamdoon A.
Agabein, Eiman ME.
Alqasoomi, Ibrahim
Alrashidi, Salem A.
Sajid, Mohammed S. M.
Mobark, Mugahid A.
author_sort Eltom, Salah E. M.
collection PubMed
description The ostrich oil of Struthio camelus (Ratite) found uses in folk medicine as an anti-inflammatory in eczema and contact dermatitis. The anti-inflammatory effect of a γ-lactone (5-hexyl-3H-furan-2-one) isolated from ostrich oil and its formulated nano-emulsion in formalin-induced paw edema was investigated in this study. Ostrich oil was saponified using a standard procedure; the aqueous residue was fractionated, purified, and characterized as γ-lactone (5-hexyl-3H-furan-2-one) through the interpretation of IR, NMR, and MS analyses. The γ-lactone was formulated as nano-emulsion using methylcellulose (MC) for oral solubilized form. The γ-lactone methylcellulose nanoparticles (γ-lactone-MC-NPs) were characterized for their size, shape, and encapsulation efficiency with a uniform size of 300 nm and 59.9% drug content. The γ-lactone was applied topically, while the formulated nanoparticles (NPs) were administered orally to rats. A non-steroidal anti-inflammatory drug (diclofenac gel) was used as a reference drug for topical use and ibuprofen suspension for oral administration. Edema was measured using the plethysmograph method. Both γ-lactone and γ-lactone-MC-NPs showed reduction of formalin-induced paw edema in rats and proved to be better than the reference drugs; diclofenac gel and ibuprofen emulsion. Histological examination of the skin tissue revealed increased skin thickness with subepidermal edema and mixed inflammatory cellular infiltration, which were significantly reduced by the γ-lactone compared to the positive control (p-value = 0.00013). Diuretic and toxicity studies of oral γ-lactone-MC-NPs were performed. No diuretic activity was observed. However, lethargy, drowsiness, and refusal to feeding observed may limit its oral administration.
format Online
Article
Text
id pubmed-8234909
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82349092021-06-27 The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema Eltom, Salah E. M. Abdellatif, Ahmed A. H. Maswadeh, Hamzah Al-Omar, Mohsen S. Abdel-Hafez, Atef A. Mohammed, Hamdoon A. Agabein, Eiman ME. Alqasoomi, Ibrahim Alrashidi, Salem A. Sajid, Mohammed S. M. Mobark, Mugahid A. Molecules Article The ostrich oil of Struthio camelus (Ratite) found uses in folk medicine as an anti-inflammatory in eczema and contact dermatitis. The anti-inflammatory effect of a γ-lactone (5-hexyl-3H-furan-2-one) isolated from ostrich oil and its formulated nano-emulsion in formalin-induced paw edema was investigated in this study. Ostrich oil was saponified using a standard procedure; the aqueous residue was fractionated, purified, and characterized as γ-lactone (5-hexyl-3H-furan-2-one) through the interpretation of IR, NMR, and MS analyses. The γ-lactone was formulated as nano-emulsion using methylcellulose (MC) for oral solubilized form. The γ-lactone methylcellulose nanoparticles (γ-lactone-MC-NPs) were characterized for their size, shape, and encapsulation efficiency with a uniform size of 300 nm and 59.9% drug content. The γ-lactone was applied topically, while the formulated nanoparticles (NPs) were administered orally to rats. A non-steroidal anti-inflammatory drug (diclofenac gel) was used as a reference drug for topical use and ibuprofen suspension for oral administration. Edema was measured using the plethysmograph method. Both γ-lactone and γ-lactone-MC-NPs showed reduction of formalin-induced paw edema in rats and proved to be better than the reference drugs; diclofenac gel and ibuprofen emulsion. Histological examination of the skin tissue revealed increased skin thickness with subepidermal edema and mixed inflammatory cellular infiltration, which were significantly reduced by the γ-lactone compared to the positive control (p-value = 0.00013). Diuretic and toxicity studies of oral γ-lactone-MC-NPs were performed. No diuretic activity was observed. However, lethargy, drowsiness, and refusal to feeding observed may limit its oral administration. MDPI 2021-06-17 /pmc/articles/PMC8234909/ /pubmed/34204472 http://dx.doi.org/10.3390/molecules26123701 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eltom, Salah E. M.
Abdellatif, Ahmed A. H.
Maswadeh, Hamzah
Al-Omar, Mohsen S.
Abdel-Hafez, Atef A.
Mohammed, Hamdoon A.
Agabein, Eiman ME.
Alqasoomi, Ibrahim
Alrashidi, Salem A.
Sajid, Mohammed S. M.
Mobark, Mugahid A.
The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema
title The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema
title_full The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema
title_fullStr The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema
title_full_unstemmed The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema
title_short The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema
title_sort anti-inflammatory effect of a γ-lactone isolated from ostrich oil of struthio camelus (ratite) and its formulated nano-emulsion in formalin-induced paw edema
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234909/
https://www.ncbi.nlm.nih.gov/pubmed/34204472
http://dx.doi.org/10.3390/molecules26123701
work_keys_str_mv AT eltomsalahem theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT abdellatifahmedah theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT maswadehhamzah theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT alomarmohsens theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT abdelhafezatefa theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT mohammedhamdoona theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT agabeineimanme theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT alqasoomiibrahim theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT alrashidisalema theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT sajidmohammedsm theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT mobarkmugahida theantiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT eltomsalahem antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT abdellatifahmedah antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT maswadehhamzah antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT alomarmohsens antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT abdelhafezatefa antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT mohammedhamdoona antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT agabeineimanme antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT alqasoomiibrahim antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT alrashidisalema antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT sajidmohammedsm antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema
AT mobarkmugahida antiinflammatoryeffectofaglactoneisolatedfromostrichoilofstruthiocamelusratiteanditsformulatednanoemulsioninformalininducedpawedema