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The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis

Intracellular lipid body (LB) biogenesis depends on the symbiosis between coral hosts and their Symbiodinaceae. Therefore, understanding the mechanism(s) behind LB biosynthesis in corals can portentially elucide the drivers of cellular regulation during endosymbiosis. This study assessed LB formatio...

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Autores principales: Chen, Hung-Kai, Rosset, Sabrina L., Wang, Li-Hsueh, Chen, Chii-Shiarng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234918/
https://www.ncbi.nlm.nih.gov/pubmed/34221732
http://dx.doi.org/10.7717/peerj.11652
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author Chen, Hung-Kai
Rosset, Sabrina L.
Wang, Li-Hsueh
Chen, Chii-Shiarng
author_facet Chen, Hung-Kai
Rosset, Sabrina L.
Wang, Li-Hsueh
Chen, Chii-Shiarng
author_sort Chen, Hung-Kai
collection PubMed
description Intracellular lipid body (LB) biogenesis depends on the symbiosis between coral hosts and their Symbiodinaceae. Therefore, understanding the mechanism(s) behind LB biosynthesis in corals can portentially elucide the drivers of cellular regulation during endosymbiosis. This study assessed LB formation in the gastrodermal tissue layer of the hermatypic coral Euphyllia glabrescens. Diel rhythmicity in LB size and distribution was observed; solar irradiation onset at sunrise initiated an increase in LB formation, which continued throughout the day and peaked after sunset at 18:00. The LBs migrated from the area near the mesoglea to the gastrodermal cell border near the coelenteron. Micro-LB biogenesis occurred in the endoplasmic reticulum (ER) of the host gastrodermal cells. A transcriptomic analysis of genes related to lipogenesis indicated that binding immunoglobulin protein (BiP) plays a key role in metabolic signaling pathways. The diel rhythmicity of LB biogenesis was correlated with ER-localized BiP expression. BiP expression peaked during the period with the largest increase in LB formation, thereby indicating that the chaperoning reaction of abnormal protein folding inside the host ER is likely involved in LB biosynthesis. These findings suggest that the host ER, central to LB formation, potentially facilitates the regulation of endosymbiosis between coral hosts and Symbiodiniaceae.
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spelling pubmed-82349182021-07-02 The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis Chen, Hung-Kai Rosset, Sabrina L. Wang, Li-Hsueh Chen, Chii-Shiarng PeerJ Cell Biology Intracellular lipid body (LB) biogenesis depends on the symbiosis between coral hosts and their Symbiodinaceae. Therefore, understanding the mechanism(s) behind LB biosynthesis in corals can portentially elucide the drivers of cellular regulation during endosymbiosis. This study assessed LB formation in the gastrodermal tissue layer of the hermatypic coral Euphyllia glabrescens. Diel rhythmicity in LB size and distribution was observed; solar irradiation onset at sunrise initiated an increase in LB formation, which continued throughout the day and peaked after sunset at 18:00. The LBs migrated from the area near the mesoglea to the gastrodermal cell border near the coelenteron. Micro-LB biogenesis occurred in the endoplasmic reticulum (ER) of the host gastrodermal cells. A transcriptomic analysis of genes related to lipogenesis indicated that binding immunoglobulin protein (BiP) plays a key role in metabolic signaling pathways. The diel rhythmicity of LB biogenesis was correlated with ER-localized BiP expression. BiP expression peaked during the period with the largest increase in LB formation, thereby indicating that the chaperoning reaction of abnormal protein folding inside the host ER is likely involved in LB biosynthesis. These findings suggest that the host ER, central to LB formation, potentially facilitates the regulation of endosymbiosis between coral hosts and Symbiodiniaceae. PeerJ Inc. 2021-06-23 /pmc/articles/PMC8234918/ /pubmed/34221732 http://dx.doi.org/10.7717/peerj.11652 Text en ©2021 Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Chen, Hung-Kai
Rosset, Sabrina L.
Wang, Li-Hsueh
Chen, Chii-Shiarng
The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis
title The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis
title_full The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis
title_fullStr The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis
title_full_unstemmed The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis
title_short The characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis
title_sort characteristics of host lipid body biogenesis during coral-dinoflagellate endosymbiosis
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8234918/
https://www.ncbi.nlm.nih.gov/pubmed/34221732
http://dx.doi.org/10.7717/peerj.11652
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