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Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine

Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of h...

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Autores principales: Agrati, Chiara, Castilletti, Concetta, Goletti, Delia, Meschi, Silvia, Sacchi, Alessandra, Matusali, Giulia, Bordoni, Veronica, Petrone, Linda, Lapa, Daniele, Notari, Stefania, Vanini, Valentina, Colavita, Francesca, Aiello, Alessandra, Agresta, Alessandro, Farroni, Chiara, Grassi, Germana, Leone, Sara, Vaia, Francesco, Capobianchi, Maria Rosaria, Ippolito, Giuseppe, Puro, Vincenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235087/
https://www.ncbi.nlm.nih.gov/pubmed/34208751
http://dx.doi.org/10.3390/microorganisms9061315
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author Agrati, Chiara
Castilletti, Concetta
Goletti, Delia
Meschi, Silvia
Sacchi, Alessandra
Matusali, Giulia
Bordoni, Veronica
Petrone, Linda
Lapa, Daniele
Notari, Stefania
Vanini, Valentina
Colavita, Francesca
Aiello, Alessandra
Agresta, Alessandro
Farroni, Chiara
Grassi, Germana
Leone, Sara
Vaia, Francesco
Capobianchi, Maria Rosaria
Ippolito, Giuseppe
Puro, Vincenzo
author_facet Agrati, Chiara
Castilletti, Concetta
Goletti, Delia
Meschi, Silvia
Sacchi, Alessandra
Matusali, Giulia
Bordoni, Veronica
Petrone, Linda
Lapa, Daniele
Notari, Stefania
Vanini, Valentina
Colavita, Francesca
Aiello, Alessandra
Agresta, Alessandro
Farroni, Chiara
Grassi, Germana
Leone, Sara
Vaia, Francesco
Capobianchi, Maria Rosaria
Ippolito, Giuseppe
Puro, Vincenzo
author_sort Agrati, Chiara
collection PubMed
description Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of health care workers (HCWs) who received a two-dose BNT162b2-mRNA vaccination. The serological response was evaluated by quantifying the anti-RBD and neutralizing antibodies. The cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-γ, TNF-α, IL-2), produced in response to spike peptides. The BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune responses against spike peptides in virtually all HCWs without previous SARS-CoV-2 infection, with a moderate inverse relation with age in the anti-RBD response. Spike-specific T cells produced several Th1 cytokines (IFN-γ, TNF-α, and IL-2), which correlated with the specific-serological response. Overall, our study describes the ability of the BNT162b2 mRNA vaccine to elicit a coordinated neutralizing humoral and spike-specific T cell response in HCWs. Assessing the dynamics of these parameters by an easy immune monitoring protocol can allow for the evaluation of the persistence of the vaccine response in order to define the optimal vaccination strategy.
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spelling pubmed-82350872021-06-27 Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine Agrati, Chiara Castilletti, Concetta Goletti, Delia Meschi, Silvia Sacchi, Alessandra Matusali, Giulia Bordoni, Veronica Petrone, Linda Lapa, Daniele Notari, Stefania Vanini, Valentina Colavita, Francesca Aiello, Alessandra Agresta, Alessandro Farroni, Chiara Grassi, Germana Leone, Sara Vaia, Francesco Capobianchi, Maria Rosaria Ippolito, Giuseppe Puro, Vincenzo Microorganisms Brief Report Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of health care workers (HCWs) who received a two-dose BNT162b2-mRNA vaccination. The serological response was evaluated by quantifying the anti-RBD and neutralizing antibodies. The cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-γ, TNF-α, IL-2), produced in response to spike peptides. The BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune responses against spike peptides in virtually all HCWs without previous SARS-CoV-2 infection, with a moderate inverse relation with age in the anti-RBD response. Spike-specific T cells produced several Th1 cytokines (IFN-γ, TNF-α, and IL-2), which correlated with the specific-serological response. Overall, our study describes the ability of the BNT162b2 mRNA vaccine to elicit a coordinated neutralizing humoral and spike-specific T cell response in HCWs. Assessing the dynamics of these parameters by an easy immune monitoring protocol can allow for the evaluation of the persistence of the vaccine response in order to define the optimal vaccination strategy. MDPI 2021-06-16 /pmc/articles/PMC8235087/ /pubmed/34208751 http://dx.doi.org/10.3390/microorganisms9061315 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Agrati, Chiara
Castilletti, Concetta
Goletti, Delia
Meschi, Silvia
Sacchi, Alessandra
Matusali, Giulia
Bordoni, Veronica
Petrone, Linda
Lapa, Daniele
Notari, Stefania
Vanini, Valentina
Colavita, Francesca
Aiello, Alessandra
Agresta, Alessandro
Farroni, Chiara
Grassi, Germana
Leone, Sara
Vaia, Francesco
Capobianchi, Maria Rosaria
Ippolito, Giuseppe
Puro, Vincenzo
Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine
title Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine
title_full Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine
title_fullStr Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine
title_full_unstemmed Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine
title_short Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine
title_sort coordinate induction of humoral and spike specific t-cell response in a cohort of italian health care workers receiving bnt162b2 mrna vaccine
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235087/
https://www.ncbi.nlm.nih.gov/pubmed/34208751
http://dx.doi.org/10.3390/microorganisms9061315
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