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Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis

Wedelia chinensis, which belongs to the Asteraceae family, is a procumbent, perennial herb. It has medicinal anti-inflammatory properties and has been traditionally used as folk medicine in East and South Asia for treating fever, cough and phlegm. In Taiwan, W. chinensis is a common ingredient of he...

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Autores principales: Tsai, Yung-Ting, Chen, Po-Yen, To, Kin-Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235177/
https://www.ncbi.nlm.nih.gov/pubmed/34204356
http://dx.doi.org/10.3390/plants10061232
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author Tsai, Yung-Ting
Chen, Po-Yen
To, Kin-Ying
author_facet Tsai, Yung-Ting
Chen, Po-Yen
To, Kin-Ying
author_sort Tsai, Yung-Ting
collection PubMed
description Wedelia chinensis, which belongs to the Asteraceae family, is a procumbent, perennial herb. It has medicinal anti-inflammatory properties and has been traditionally used as folk medicine in East and South Asia for treating fever, cough and phlegm. In Taiwan, W. chinensis is a common ingredient of herbal tea. Previous studies showed that the plant leaves contain four major bioactive compounds, wedelolactone, demethylwedelolactone, luteolin and apigenin, that have potent antihepatoxic activity, and are thus used as major ingredients in phytopharmaceutical formulations. In this study, we set up optimal conditions for induction of ploidy in W. chinensis. Ploidy can be an effective method of increasing plant biomass and improving medicinal and ornamental characteristics. By using flow cytometry and chicken erythrocyte nuclei as a reference, the DNA content (2C) or genome size of W. chinensis was determined to be 4.80 picograms (pg) in this study for the first time. Subsequently, we developed the successful induction of five triploid and three tetraploid plants by using shoot explants treated with different concentrations (0, 0.25, 0.5, 1, 1.5, 2 g/L) of colchicine. No apparent morphological changes were observed between these polyploid plants and the diploid wild-type (WT) plant, except that larger stomata in leaves were found in all polyploid plants as compared to diploid WT. Ultra-performance liquid chromatography coupled with tandem mass spectrometry was used to quantify the four index compounds (wedelolactone, demethylwedelolactone, luteolin, apigenin) in these polyploid plants, and fluctuating patterns were detected. This is the first report regarding polyploidy in the herbal plant W. chinensis.
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spelling pubmed-82351772021-06-27 Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis Tsai, Yung-Ting Chen, Po-Yen To, Kin-Ying Plants (Basel) Article Wedelia chinensis, which belongs to the Asteraceae family, is a procumbent, perennial herb. It has medicinal anti-inflammatory properties and has been traditionally used as folk medicine in East and South Asia for treating fever, cough and phlegm. In Taiwan, W. chinensis is a common ingredient of herbal tea. Previous studies showed that the plant leaves contain four major bioactive compounds, wedelolactone, demethylwedelolactone, luteolin and apigenin, that have potent antihepatoxic activity, and are thus used as major ingredients in phytopharmaceutical formulations. In this study, we set up optimal conditions for induction of ploidy in W. chinensis. Ploidy can be an effective method of increasing plant biomass and improving medicinal and ornamental characteristics. By using flow cytometry and chicken erythrocyte nuclei as a reference, the DNA content (2C) or genome size of W. chinensis was determined to be 4.80 picograms (pg) in this study for the first time. Subsequently, we developed the successful induction of five triploid and three tetraploid plants by using shoot explants treated with different concentrations (0, 0.25, 0.5, 1, 1.5, 2 g/L) of colchicine. No apparent morphological changes were observed between these polyploid plants and the diploid wild-type (WT) plant, except that larger stomata in leaves were found in all polyploid plants as compared to diploid WT. Ultra-performance liquid chromatography coupled with tandem mass spectrometry was used to quantify the four index compounds (wedelolactone, demethylwedelolactone, luteolin, apigenin) in these polyploid plants, and fluctuating patterns were detected. This is the first report regarding polyploidy in the herbal plant W. chinensis. MDPI 2021-06-17 /pmc/articles/PMC8235177/ /pubmed/34204356 http://dx.doi.org/10.3390/plants10061232 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Yung-Ting
Chen, Po-Yen
To, Kin-Ying
Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis
title Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis
title_full Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis
title_fullStr Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis
title_full_unstemmed Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis
title_short Induction of Polyploidy and Metabolic Profiling in the Medicinal Herb Wedelia chinensis
title_sort induction of polyploidy and metabolic profiling in the medicinal herb wedelia chinensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235177/
https://www.ncbi.nlm.nih.gov/pubmed/34204356
http://dx.doi.org/10.3390/plants10061232
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