The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide

In this paper, we present findings from studying the interaction of copper(II) ions with the His(2)-cyclopentapeptide and the role of proline used for the purpose of potentiometric titration and UV-Vis, CD and EPR spectroscopic measurements. Experiments of two homodetic peptides differing by one ami...

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Autores principales: Pieniężna, Aleksandra, Kotynia, Aleksandra, Brasuń, Justyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235444/
https://www.ncbi.nlm.nih.gov/pubmed/34205682
http://dx.doi.org/10.3390/ijms22126628
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author Pieniężna, Aleksandra
Kotynia, Aleksandra
Brasuń, Justyna
author_facet Pieniężna, Aleksandra
Kotynia, Aleksandra
Brasuń, Justyna
author_sort Pieniężna, Aleksandra
collection PubMed
description In this paper, we present findings from studying the interaction of copper(II) ions with the His(2)-cyclopentapeptide and the role of proline used for the purpose of potentiometric titration and UV-Vis, CD and EPR spectroscopic measurements. Experiments of two homodetic peptides differing by one amino acid residue were conducted for a ligand to metal ratio of 1:1 in the pH range 2.5–11.0. The presented studies reveal that peptides form only mononuclear complexes, and the CuH(2)L complex appears in the system first (for both L1 and L2). Study results show that the presence of Pro influences the structure of formed complexes and their stabilities and has a strong impact on the efficiency of copper(II) coordination.
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spelling pubmed-82354442021-06-27 The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide Pieniężna, Aleksandra Kotynia, Aleksandra Brasuń, Justyna Int J Mol Sci Article In this paper, we present findings from studying the interaction of copper(II) ions with the His(2)-cyclopentapeptide and the role of proline used for the purpose of potentiometric titration and UV-Vis, CD and EPR spectroscopic measurements. Experiments of two homodetic peptides differing by one amino acid residue were conducted for a ligand to metal ratio of 1:1 in the pH range 2.5–11.0. The presented studies reveal that peptides form only mononuclear complexes, and the CuH(2)L complex appears in the system first (for both L1 and L2). Study results show that the presence of Pro influences the structure of formed complexes and their stabilities and has a strong impact on the efficiency of copper(II) coordination. MDPI 2021-06-21 /pmc/articles/PMC8235444/ /pubmed/34205682 http://dx.doi.org/10.3390/ijms22126628 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pieniężna, Aleksandra
Kotynia, Aleksandra
Brasuń, Justyna
The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide
title The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide
title_full The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide
title_fullStr The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide
title_full_unstemmed The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide
title_short The Unusual Role of Pro in Cu(II) Binding by His(2)-Cyclopentapeptide
title_sort unusual role of pro in cu(ii) binding by his(2)-cyclopentapeptide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235444/
https://www.ncbi.nlm.nih.gov/pubmed/34205682
http://dx.doi.org/10.3390/ijms22126628
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