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Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland
The wild boar is the most important reservoir of zoonotic HEV-3 strains among different wildlife species. The aim of the study was subtype identification of wild boar HEV-3 strains circulating in Poland. Wild boar liver was used in the study in the form of homogenates prepared from 57 samples positi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235543/ https://www.ncbi.nlm.nih.gov/pubmed/34205456 http://dx.doi.org/10.3390/v13061176 |
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author | Kozyra, Iwona Bigoraj, Ewelina Jabłoński, Artur Politi, Katerina Rzeżutka, Artur |
author_facet | Kozyra, Iwona Bigoraj, Ewelina Jabłoński, Artur Politi, Katerina Rzeżutka, Artur |
author_sort | Kozyra, Iwona |
collection | PubMed |
description | The wild boar is the most important reservoir of zoonotic HEV-3 strains among different wildlife species. The aim of the study was subtype identification of wild boar HEV-3 strains circulating in Poland. Wild boar liver was used in the study in the form of homogenates prepared from 57 samples positive for HEV in a real-time RT-PCR. These samples were collected from juvenile and adult wild boars hunted in the jurisdictions of different Regional Directorates of State Forests (RDSF) across Poland. Subtype identification of detected HEV strains was based on a phylogenetic analysis of the most conserved HEV ORF2 genome fragment. Out of 57 tested samples, consensus HEV ORF2 sequences of 348 bp were obtained for 45 strains. Nineteen strains were identified and belonged to the HEV gt 3a and 3i subtypes, whereas 26 were not assigned to any virus subtype. HEV gt 3i strains prevailed in the Polish wild boar population, 16 of such were identified, and they were significantly more often observed in the RDSF Katowice area (χ(2) = 28.6, p = 0.027 (<0.05)) compared to other regions of the country. Circulation of 3a strains was limited only to the RDSF Gdańsk territory (χ(2) = 48, p = 0.000 (<0.05)). The virus strains detected in the Polish population of wild boars representing previously identified HEV subtypes in wild boars, pigs, or humans in Europe are of epidemiological importance for public health. |
format | Online Article Text |
id | pubmed-8235543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82355432021-06-27 Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland Kozyra, Iwona Bigoraj, Ewelina Jabłoński, Artur Politi, Katerina Rzeżutka, Artur Viruses Brief Report The wild boar is the most important reservoir of zoonotic HEV-3 strains among different wildlife species. The aim of the study was subtype identification of wild boar HEV-3 strains circulating in Poland. Wild boar liver was used in the study in the form of homogenates prepared from 57 samples positive for HEV in a real-time RT-PCR. These samples were collected from juvenile and adult wild boars hunted in the jurisdictions of different Regional Directorates of State Forests (RDSF) across Poland. Subtype identification of detected HEV strains was based on a phylogenetic analysis of the most conserved HEV ORF2 genome fragment. Out of 57 tested samples, consensus HEV ORF2 sequences of 348 bp were obtained for 45 strains. Nineteen strains were identified and belonged to the HEV gt 3a and 3i subtypes, whereas 26 were not assigned to any virus subtype. HEV gt 3i strains prevailed in the Polish wild boar population, 16 of such were identified, and they were significantly more often observed in the RDSF Katowice area (χ(2) = 28.6, p = 0.027 (<0.05)) compared to other regions of the country. Circulation of 3a strains was limited only to the RDSF Gdańsk territory (χ(2) = 48, p = 0.000 (<0.05)). The virus strains detected in the Polish population of wild boars representing previously identified HEV subtypes in wild boars, pigs, or humans in Europe are of epidemiological importance for public health. MDPI 2021-06-19 /pmc/articles/PMC8235543/ /pubmed/34205456 http://dx.doi.org/10.3390/v13061176 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Kozyra, Iwona Bigoraj, Ewelina Jabłoński, Artur Politi, Katerina Rzeżutka, Artur Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland |
title | Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland |
title_full | Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland |
title_fullStr | Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland |
title_full_unstemmed | Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland |
title_short | Genetic Diversity and Epidemiological Significance of Wild Boar HEV-3 Strains Circulating in Poland |
title_sort | genetic diversity and epidemiological significance of wild boar hev-3 strains circulating in poland |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235543/ https://www.ncbi.nlm.nih.gov/pubmed/34205456 http://dx.doi.org/10.3390/v13061176 |
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