Cargando…
Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay
OBJECTIVE: LIA-ANA-Profile-17S is a multiplex line immunoassay that simultaneously detects 17 antinuclear antibodies (ANAs) against extractable nuclear antigens (ENAs). We evaluated the utility of LIA-ANA-Profile-17S as a supplement to ANA indirect immunofluorescence (IIF) and EliA ENA (a fluorescen...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236799/ https://www.ncbi.nlm.nih.gov/pubmed/34154430 http://dx.doi.org/10.1177/03000605211014390 |
_version_ | 1783714617689636864 |
---|---|
author | Jang, Jaehyeok Kim, Sinyoung Kim, Hyon-Suk Lee, Kyung-A Park, Jungyong Park, Younhee |
author_facet | Jang, Jaehyeok Kim, Sinyoung Kim, Hyon-Suk Lee, Kyung-A Park, Jungyong Park, Younhee |
author_sort | Jang, Jaehyeok |
collection | PubMed |
description | OBJECTIVE: LIA-ANA-Profile-17S is a multiplex line immunoassay that simultaneously detects 17 antinuclear antibodies (ANAs) against extractable nuclear antigens (ENAs). We evaluated the utility of LIA-ANA-Profile-17S as a supplement to ANA indirect immunofluorescence (IIF) and EliA ENA (a fluorescence enzyme immunoassay) for diagnosis of ANA-associated rheumatic diseases. METHODS: Sera were collected from 245 patients referred for an ANA IIF test. LIA-ANA-Profile-17S results were compared with those of EliA ENA. The kappa coefficients, agreement rates, and diagnostic performance of these tests were assessed for systemic lupus erythematosus (SLE) and Sjögren’s syndrome (SjS). RESULTS: We observed almost perfect interassay agreement for antibodies against Ro52/Ro60, CENP-B, and Scl-70 (kappa = 0.91, 0.97, and 1.00, respectively); strong agreement for anti-SS-B/La antibody (kappa = 0.81); and relatively low agreement for other antibodies, including those against dsDNA, Sm, RNP, and Jo-1. For SLE diagnosis, LIA-ANA-Profile-17S showed lower sensitivity and similar specificity compared with EliA ENA. The sensitivity and specificity of these two assays were similar for SjS diagnosis. CONCLUSIONS: The specificity of LIA-ANA-Profile-17S was enhanced when combined with ANA IIF and was comparable with that of EliA ENA. LIA-ANA-Profile-17S showed relatively good agreement with EliA ENA. In combination with ANA IIF, these assays showed enhanced diagnostic performance. |
format | Online Article Text |
id | pubmed-8236799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-82367992021-07-08 Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay Jang, Jaehyeok Kim, Sinyoung Kim, Hyon-Suk Lee, Kyung-A Park, Jungyong Park, Younhee J Int Med Res Retrospective Clinical Research Report OBJECTIVE: LIA-ANA-Profile-17S is a multiplex line immunoassay that simultaneously detects 17 antinuclear antibodies (ANAs) against extractable nuclear antigens (ENAs). We evaluated the utility of LIA-ANA-Profile-17S as a supplement to ANA indirect immunofluorescence (IIF) and EliA ENA (a fluorescence enzyme immunoassay) for diagnosis of ANA-associated rheumatic diseases. METHODS: Sera were collected from 245 patients referred for an ANA IIF test. LIA-ANA-Profile-17S results were compared with those of EliA ENA. The kappa coefficients, agreement rates, and diagnostic performance of these tests were assessed for systemic lupus erythematosus (SLE) and Sjögren’s syndrome (SjS). RESULTS: We observed almost perfect interassay agreement for antibodies against Ro52/Ro60, CENP-B, and Scl-70 (kappa = 0.91, 0.97, and 1.00, respectively); strong agreement for anti-SS-B/La antibody (kappa = 0.81); and relatively low agreement for other antibodies, including those against dsDNA, Sm, RNP, and Jo-1. For SLE diagnosis, LIA-ANA-Profile-17S showed lower sensitivity and similar specificity compared with EliA ENA. The sensitivity and specificity of these two assays were similar for SjS diagnosis. CONCLUSIONS: The specificity of LIA-ANA-Profile-17S was enhanced when combined with ANA IIF and was comparable with that of EliA ENA. LIA-ANA-Profile-17S showed relatively good agreement with EliA ENA. In combination with ANA IIF, these assays showed enhanced diagnostic performance. SAGE Publications 2021-06-22 /pmc/articles/PMC8236799/ /pubmed/34154430 http://dx.doi.org/10.1177/03000605211014390 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Retrospective Clinical Research Report Jang, Jaehyeok Kim, Sinyoung Kim, Hyon-Suk Lee, Kyung-A Park, Jungyong Park, Younhee Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay |
title | Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay |
title_full | Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay |
title_fullStr | Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay |
title_full_unstemmed | Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay |
title_short | Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay |
title_sort | comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay |
topic | Retrospective Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236799/ https://www.ncbi.nlm.nih.gov/pubmed/34154430 http://dx.doi.org/10.1177/03000605211014390 |
work_keys_str_mv | AT jangjaehyeok comparisonofantinuclearantibodyprofilesobtainedusinglineimmunoassayandfluorescenceenzymeimmunoassay AT kimsinyoung comparisonofantinuclearantibodyprofilesobtainedusinglineimmunoassayandfluorescenceenzymeimmunoassay AT kimhyonsuk comparisonofantinuclearantibodyprofilesobtainedusinglineimmunoassayandfluorescenceenzymeimmunoassay AT leekyunga comparisonofantinuclearantibodyprofilesobtainedusinglineimmunoassayandfluorescenceenzymeimmunoassay AT parkjungyong comparisonofantinuclearantibodyprofilesobtainedusinglineimmunoassayandfluorescenceenzymeimmunoassay AT parkyounhee comparisonofantinuclearantibodyprofilesobtainedusinglineimmunoassayandfluorescenceenzymeimmunoassay |