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Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool

The t‐soluble NSF‐attachment protein receptor protein Syntaxin‐1a (Stx‐1a) is abundantly expressed at pre‐synaptic terminals where it plays a critical role in the exocytosis of neurotransmitter‐containing synaptic vesicles. Stx‐1a is phosphorylated by Casein kinase 2α (CK2α) at Ser14, which has been...

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Autores principales: Shi, Vanilla (Hua), Craig, Tim J., Bishop, Paul, Nakamura, Yasuko, Rocca, Dan, Wilkinson, Kevin A., Henley, Jeremy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237229/
https://www.ncbi.nlm.nih.gov/pubmed/32852799
http://dx.doi.org/10.1111/jnc.15161
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author Shi, Vanilla (Hua)
Craig, Tim J.
Bishop, Paul
Nakamura, Yasuko
Rocca, Dan
Wilkinson, Kevin A.
Henley, Jeremy M.
author_facet Shi, Vanilla (Hua)
Craig, Tim J.
Bishop, Paul
Nakamura, Yasuko
Rocca, Dan
Wilkinson, Kevin A.
Henley, Jeremy M.
author_sort Shi, Vanilla (Hua)
collection PubMed
description The t‐soluble NSF‐attachment protein receptor protein Syntaxin‐1a (Stx‐1a) is abundantly expressed at pre‐synaptic terminals where it plays a critical role in the exocytosis of neurotransmitter‐containing synaptic vesicles. Stx‐1a is phosphorylated by Casein kinase 2α (CK2α) at Ser14, which has been proposed to regulate the interaction of Stx‐1a and Munc‐18 to control of synaptic vesicle priming. However, the role of CK2α in synaptic vesicle dynamics remains unclear. Here, we show that CK2α over‐expression reduces evoked synaptic vesicle release. Furthermore, shRNA‐mediated knockdown of CK2α in primary hippocampal neurons strongly enhanced vesicle exocytosis from the reserve pool, with no effect on the readily releasable pool of primed vesicles. In neurons in which endogenous Stx‐1a was knocked down and replaced with a CK2α phosphorylation‐deficient mutant, Stx‐1a(D17A), vesicle exocytosis was also increased. These results reveal a previously unsuspected role of CK2α phosphorylation in specifically regulating the reserve synaptic vesicle pool, without changing the kinetics of release from the readily releasable pool. [Image: see text]
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spelling pubmed-82372292021-06-29 Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool Shi, Vanilla (Hua) Craig, Tim J. Bishop, Paul Nakamura, Yasuko Rocca, Dan Wilkinson, Kevin A. Henley, Jeremy M. J Neurochem ORIGINAL ARTICLES The t‐soluble NSF‐attachment protein receptor protein Syntaxin‐1a (Stx‐1a) is abundantly expressed at pre‐synaptic terminals where it plays a critical role in the exocytosis of neurotransmitter‐containing synaptic vesicles. Stx‐1a is phosphorylated by Casein kinase 2α (CK2α) at Ser14, which has been proposed to regulate the interaction of Stx‐1a and Munc‐18 to control of synaptic vesicle priming. However, the role of CK2α in synaptic vesicle dynamics remains unclear. Here, we show that CK2α over‐expression reduces evoked synaptic vesicle release. Furthermore, shRNA‐mediated knockdown of CK2α in primary hippocampal neurons strongly enhanced vesicle exocytosis from the reserve pool, with no effect on the readily releasable pool of primed vesicles. In neurons in which endogenous Stx‐1a was knocked down and replaced with a CK2α phosphorylation‐deficient mutant, Stx‐1a(D17A), vesicle exocytosis was also increased. These results reveal a previously unsuspected role of CK2α phosphorylation in specifically regulating the reserve synaptic vesicle pool, without changing the kinetics of release from the readily releasable pool. [Image: see text] John Wiley and Sons Inc. 2020-09-06 2021-03 /pmc/articles/PMC8237229/ /pubmed/32852799 http://dx.doi.org/10.1111/jnc.15161 Text en © 2020 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle ORIGINAL ARTICLES
Shi, Vanilla (Hua)
Craig, Tim J.
Bishop, Paul
Nakamura, Yasuko
Rocca, Dan
Wilkinson, Kevin A.
Henley, Jeremy M.
Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool
title Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool
title_full Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool
title_fullStr Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool
title_full_unstemmed Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool
title_short Phosphorylation of Syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool
title_sort phosphorylation of syntaxin‐1a by casein kinase 2α regulates pre‐synaptic vesicle exocytosis from the reserve pool
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237229/
https://www.ncbi.nlm.nih.gov/pubmed/32852799
http://dx.doi.org/10.1111/jnc.15161
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