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Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor Anxa5 in the pituitary gland of female rats
Functional relationship between nuclear receptor subfamily 4 group A member 3 (Nr4a3) and annexin A5 (Anxa5), which are two gonadotropin-releasing hormone (GnRH)-inducible genes, has been established while evaluating pituitary gonadotropes in relation to follicle-stimulating hormone beta (Fshb) expr...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Society for Reproduction and Development
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238674/ https://www.ncbi.nlm.nih.gov/pubmed/33840679 http://dx.doi.org/10.1262/jrd.2021-029 |
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author | TERASHIMA, Ryota LAOHARATCHATATHANIN, Titaree KURUSU, Shiro KAWAMINAMI, Mitsumori |
author_facet | TERASHIMA, Ryota LAOHARATCHATATHANIN, Titaree KURUSU, Shiro KAWAMINAMI, Mitsumori |
author_sort | TERASHIMA, Ryota |
collection | PubMed |
description | Functional relationship between nuclear receptor subfamily 4 group A member 3 (Nr4a3) and annexin A5 (Anxa5), which are two gonadotropin-releasing hormone (GnRH)-inducible genes, has been established while evaluating pituitary gonadotropes in relation to follicle-stimulating hormone beta (Fshb) expression. However, the physiological variations that arise due to the differential expression of these genes in the pituitary gland during rat estrous cycle remain unknown. This study aimed to evaluate the Nr4a3 and Anxa5 mRNA expression during the estrous cycle in rats in comparison with the expression of the gonadotropin subunit genes, luteinizing hormone beta (Lhb) and Fshb. Nr4a3 mRNA expression showed a single peak at 1400 h of proestrus during the 4-d estrous cycle. Anxa5 mRNA level was elevated along with increased Fshb mRNA expression after the decline of Nr4a3 mRNA until 2300 h. Lhb mRNA expression levels were not significantly changed during the estrous cycle. Notably, addition of a GnRH antagonist at 1100 h completely eradicated luteinizing hormone secretion at 1400 h and 1700 h of proestrus, and significantly reduced the Nr4a3 mRNA expression level at both the time points. These results suggest that GnRH is, at least partly, responsible for the increase in pituitary Nr4a3, and that the interaction between NR4A3 and ANXA5 is required to regulate Fshb expression during the preovulatory gonadotropin surge. |
format | Online Article Text |
id | pubmed-8238674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-82386742021-07-01 Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor Anxa5 in the pituitary gland of female rats TERASHIMA, Ryota LAOHARATCHATATHANIN, Titaree KURUSU, Shiro KAWAMINAMI, Mitsumori J Reprod Dev Original Article Functional relationship between nuclear receptor subfamily 4 group A member 3 (Nr4a3) and annexin A5 (Anxa5), which are two gonadotropin-releasing hormone (GnRH)-inducible genes, has been established while evaluating pituitary gonadotropes in relation to follicle-stimulating hormone beta (Fshb) expression. However, the physiological variations that arise due to the differential expression of these genes in the pituitary gland during rat estrous cycle remain unknown. This study aimed to evaluate the Nr4a3 and Anxa5 mRNA expression during the estrous cycle in rats in comparison with the expression of the gonadotropin subunit genes, luteinizing hormone beta (Lhb) and Fshb. Nr4a3 mRNA expression showed a single peak at 1400 h of proestrus during the 4-d estrous cycle. Anxa5 mRNA level was elevated along with increased Fshb mRNA expression after the decline of Nr4a3 mRNA until 2300 h. Lhb mRNA expression levels were not significantly changed during the estrous cycle. Notably, addition of a GnRH antagonist at 1100 h completely eradicated luteinizing hormone secretion at 1400 h and 1700 h of proestrus, and significantly reduced the Nr4a3 mRNA expression level at both the time points. These results suggest that GnRH is, at least partly, responsible for the increase in pituitary Nr4a3, and that the interaction between NR4A3 and ANXA5 is required to regulate Fshb expression during the preovulatory gonadotropin surge. The Society for Reproduction and Development 2021-04-10 2021-06 /pmc/articles/PMC8238674/ /pubmed/33840679 http://dx.doi.org/10.1262/jrd.2021-029 Text en ©2021 Society for Reproduction and Development https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Original Article TERASHIMA, Ryota LAOHARATCHATATHANIN, Titaree KURUSU, Shiro KAWAMINAMI, Mitsumori Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor Anxa5 in the pituitary gland of female rats |
title | Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor
Anxa5 in the pituitary gland of female rats |
title_full | Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor
Anxa5 in the pituitary gland of female rats |
title_fullStr | Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor
Anxa5 in the pituitary gland of female rats |
title_full_unstemmed | Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor
Anxa5 in the pituitary gland of female rats |
title_short | Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor
Anxa5 in the pituitary gland of female rats |
title_sort | sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, nr4a3, and its suppressor
anxa5 in the pituitary gland of female rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238674/ https://www.ncbi.nlm.nih.gov/pubmed/33840679 http://dx.doi.org/10.1262/jrd.2021-029 |
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