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Role of Calpain-1 in Neurogenesis
While calpains have been implicated in neurogenesis for a long time, there is still little information regarding the specific contributions of various isoforms in this process. We took advantage of the availability of mutant mice with complete deletion of calpain-1 to analyze its contribution to neu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239220/ https://www.ncbi.nlm.nih.gov/pubmed/34212005 http://dx.doi.org/10.3389/fmolb.2021.685938 |
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author | Baudry, Michel Su, Wenyue Seinfeld, Jeffrey Sun, Jiandong Bi, Xiaoning |
author_facet | Baudry, Michel Su, Wenyue Seinfeld, Jeffrey Sun, Jiandong Bi, Xiaoning |
author_sort | Baudry, Michel |
collection | PubMed |
description | While calpains have been implicated in neurogenesis for a long time, there is still little information regarding the specific contributions of various isoforms in this process. We took advantage of the availability of mutant mice with complete deletion of calpain-1 to analyze its contribution to neurogenesis. We first used the incorporation of BrdU in newly-generated cells in the subgranular zone of the dentate gyrus to determine the role of calpain-1 deletion in neuronal proliferation. Our results showed that the lack of calpain-1 decreased the rate of cell proliferation in adult hippocampus. As previously shown, it also decreased the long-term survival of newly-generated neurons. We also used data from previously reported RNA and miRNA sequencing analyses to identify differentially expressed genes in brain of calpain-1 knock-out mice related to cell division, cell migration, cell proliferation and cell survival. A number of differentially expressed genes were identified, which could play a significant role in the changes in neurogenesis in calpain-1 knock out mice. The results provide new information regarding the role of calpain-1 in neurogenesis and have implications for better understanding the pathologies associated with calpain-1 mutations in humans. |
format | Online Article Text |
id | pubmed-8239220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82392202021-06-30 Role of Calpain-1 in Neurogenesis Baudry, Michel Su, Wenyue Seinfeld, Jeffrey Sun, Jiandong Bi, Xiaoning Front Mol Biosci Molecular Biosciences While calpains have been implicated in neurogenesis for a long time, there is still little information regarding the specific contributions of various isoforms in this process. We took advantage of the availability of mutant mice with complete deletion of calpain-1 to analyze its contribution to neurogenesis. We first used the incorporation of BrdU in newly-generated cells in the subgranular zone of the dentate gyrus to determine the role of calpain-1 deletion in neuronal proliferation. Our results showed that the lack of calpain-1 decreased the rate of cell proliferation in adult hippocampus. As previously shown, it also decreased the long-term survival of newly-generated neurons. We also used data from previously reported RNA and miRNA sequencing analyses to identify differentially expressed genes in brain of calpain-1 knock-out mice related to cell division, cell migration, cell proliferation and cell survival. A number of differentially expressed genes were identified, which could play a significant role in the changes in neurogenesis in calpain-1 knock out mice. The results provide new information regarding the role of calpain-1 in neurogenesis and have implications for better understanding the pathologies associated with calpain-1 mutations in humans. Frontiers Media S.A. 2021-06-15 /pmc/articles/PMC8239220/ /pubmed/34212005 http://dx.doi.org/10.3389/fmolb.2021.685938 Text en Copyright © 2021 Baudry, Su, Seinfeld, Sun and Bi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Baudry, Michel Su, Wenyue Seinfeld, Jeffrey Sun, Jiandong Bi, Xiaoning Role of Calpain-1 in Neurogenesis |
title | Role of Calpain-1 in Neurogenesis |
title_full | Role of Calpain-1 in Neurogenesis |
title_fullStr | Role of Calpain-1 in Neurogenesis |
title_full_unstemmed | Role of Calpain-1 in Neurogenesis |
title_short | Role of Calpain-1 in Neurogenesis |
title_sort | role of calpain-1 in neurogenesis |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239220/ https://www.ncbi.nlm.nih.gov/pubmed/34212005 http://dx.doi.org/10.3389/fmolb.2021.685938 |
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