Hydrophobicity of polymer based anion-exchange columns for ion chromatography

The regularities of the retention of alkanoic and alkanesulfonic acids homologues were investigated for the set of 36 anion-exchange columns produced by various manufacturers. The role of hydrophobic and electrostatic interactions in the retention and separation of organic anions was studied. The methylene selectivity increments α(CH(2)) were measured for the studied columns with 10 mM sodium hydroxide eluent. The influence of matrix, surface area, polar group structure, ion-exchange capacity, the density of charged functional groups on the surface and other characteristics of anion-exchangers on resin hydrophobicity was considered. A unified approach for the measurements of hydrophobic properties of anion-exchange resins is proposed and the ratio of chloride retention factor (k(Cl)) to α(CH(2)) was introduced as mixed-mode factor. The synergetic effect of electrostatic and hydrophobic interactions was observed.