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Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study

BACKGROUND AND AIM: Traumatic brain injury (TBI) results in death or long term functional disabilities. Eugenol is demonstrated to be beneficial in a range of experimental models of neurological disorders via its anti-inflammatory and antioxidant properties. Thus, the present study was designed to i...

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Autores principales: Barot, Jeetprakash, Saxena, Bhagawati
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240337/
https://www.ncbi.nlm.nih.gov/pubmed/34195026
http://dx.doi.org/10.1016/j.jtcme.2021.01.003
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author Barot, Jeetprakash
Saxena, Bhagawati
author_facet Barot, Jeetprakash
Saxena, Bhagawati
author_sort Barot, Jeetprakash
collection PubMed
description BACKGROUND AND AIM: Traumatic brain injury (TBI) results in death or long term functional disabilities. Eugenol is demonstrated to be beneficial in a range of experimental models of neurological disorders via its anti-inflammatory and antioxidant properties. Thus, the present study was designed to investigate the neuroprotective effects of eugenol in a weight-drop induced rat model of TBI. EXPERIMENTAL PROCEDURE: Rats were assigned into five groups; control and TBI groups pretreated with vehicle, and three TBI groups pretreated with different doses of eugenol (25, 50, and 100 mg/kg/day, p.o., seven consecutive days). Except for the control, all other groups were subjected to TBI using Marmarou’s weight-drop method. 24 h after TBI, locomotor functions and short term memory were evaluated. Lastly animals were scarified and the estimation of lipid peroxidation in brain tissue, blood-brain barrier (BBB) integrity, brain water content (brain edema) and histopathology of the brain tissue were performed. RESULTS: Weight-drop induced TBI caused functional disabilities in the rats as indicated by impairment in locomotor activities and short term memory. The TBI also resulted in augmented neuronal cell death designated by chromatolysis. The results also showed disruption in the BBB integrity, increased edema, and lipid peroxidation in the brain of the rats exposed to trauma. Pretreatment with eugenol (100 mg/kg) ameliorated histopathological, neurochemical, and behavioral consequences of trauma. CONCLUSION: For the first time this study revealed that eugenol can be considered as a potential candidate for managing the functional disabilities associated with TBI because of its antioxidant activities.
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spelling pubmed-82403372021-06-29 Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study Barot, Jeetprakash Saxena, Bhagawati J Tradit Complement Med Original Article BACKGROUND AND AIM: Traumatic brain injury (TBI) results in death or long term functional disabilities. Eugenol is demonstrated to be beneficial in a range of experimental models of neurological disorders via its anti-inflammatory and antioxidant properties. Thus, the present study was designed to investigate the neuroprotective effects of eugenol in a weight-drop induced rat model of TBI. EXPERIMENTAL PROCEDURE: Rats were assigned into five groups; control and TBI groups pretreated with vehicle, and three TBI groups pretreated with different doses of eugenol (25, 50, and 100 mg/kg/day, p.o., seven consecutive days). Except for the control, all other groups were subjected to TBI using Marmarou’s weight-drop method. 24 h after TBI, locomotor functions and short term memory were evaluated. Lastly animals were scarified and the estimation of lipid peroxidation in brain tissue, blood-brain barrier (BBB) integrity, brain water content (brain edema) and histopathology of the brain tissue were performed. RESULTS: Weight-drop induced TBI caused functional disabilities in the rats as indicated by impairment in locomotor activities and short term memory. The TBI also resulted in augmented neuronal cell death designated by chromatolysis. The results also showed disruption in the BBB integrity, increased edema, and lipid peroxidation in the brain of the rats exposed to trauma. Pretreatment with eugenol (100 mg/kg) ameliorated histopathological, neurochemical, and behavioral consequences of trauma. CONCLUSION: For the first time this study revealed that eugenol can be considered as a potential candidate for managing the functional disabilities associated with TBI because of its antioxidant activities. Elsevier 2021-01-08 /pmc/articles/PMC8240337/ /pubmed/34195026 http://dx.doi.org/10.1016/j.jtcme.2021.01.003 Text en © 2021 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Barot, Jeetprakash
Saxena, Bhagawati
Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study
title Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study
title_full Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study
title_fullStr Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study
title_full_unstemmed Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study
title_short Therapeutic effects of eugenol in a rat model of traumatic brain injury: A behavioral, biochemical, and histological study
title_sort therapeutic effects of eugenol in a rat model of traumatic brain injury: a behavioral, biochemical, and histological study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240337/
https://www.ncbi.nlm.nih.gov/pubmed/34195026
http://dx.doi.org/10.1016/j.jtcme.2021.01.003
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