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Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults

BACKGROUND: Colibactin-producing Escherichia coli containing polyketide synthase (pks(+) E. coli) has been shown to be involved in colorectal cancer (CRC) development through gut microbiota analysis in animal models. Stool status has been associated with potentially adverse gut microbiome profiles f...

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Autores principales: Watanabe, Daiki, Murakami, Haruka, Ohno, Harumi, Tanisawa, Kumpei, Konishi, Kana, Todoroki-Mori, Kikue, Tsunematsu, Yuta, Sato, Michio, Ogata, Yuji, Miyoshi, Noriyuki, Kubota, Naoto, Kunisawa, Jun, Wakabayashi, Keiji, Kubota, Tetsuya, Watanabe, Kenji, Miyachi, Motohiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240356/
https://www.ncbi.nlm.nih.gov/pubmed/34182940
http://dx.doi.org/10.1186/s12866-021-02255-6
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author Watanabe, Daiki
Murakami, Haruka
Ohno, Harumi
Tanisawa, Kumpei
Konishi, Kana
Todoroki-Mori, Kikue
Tsunematsu, Yuta
Sato, Michio
Ogata, Yuji
Miyoshi, Noriyuki
Kubota, Naoto
Kunisawa, Jun
Wakabayashi, Keiji
Kubota, Tetsuya
Watanabe, Kenji
Miyachi, Motohiko
author_facet Watanabe, Daiki
Murakami, Haruka
Ohno, Harumi
Tanisawa, Kumpei
Konishi, Kana
Todoroki-Mori, Kikue
Tsunematsu, Yuta
Sato, Michio
Ogata, Yuji
Miyoshi, Noriyuki
Kubota, Naoto
Kunisawa, Jun
Wakabayashi, Keiji
Kubota, Tetsuya
Watanabe, Kenji
Miyachi, Motohiko
author_sort Watanabe, Daiki
collection PubMed
description BACKGROUND: Colibactin-producing Escherichia coli containing polyketide synthase (pks(+) E. coli) has been shown to be involved in colorectal cancer (CRC) development through gut microbiota analysis in animal models. Stool status has been associated with potentially adverse gut microbiome profiles from fecal analysis in adults. We examined the association between stool patterns and the prevalence of pks(+) E. coli isolated from microbiota in fecal samples of 224 healthy Japanese individuals. RESULTS: Stool patterns were determined through factorial analysis using a previously validated questionnaire that included stool frequency, volume, color, shape, and odor. Factor scores were classified into tertiles. The prevalence of pks(+) E. coli was determined by using specific primers for pks(+) E. coli in fecal samples. Plasma and fecal fatty acids were measured via gas chromatography-mass spectrometry. The prevalence of pks(+) E. coli was 26.8%. Three stool patterns identified by factorial analysis accounted for 70.1% of all patterns seen (factor 1: lower frequency, darker color, and harder shape; factor 2: higher volume and softer shape; and factor 3: darker color and stronger odor). Multivariable-adjusted odds ratios (95% confidence intervals) of the prevalence of pks(+) E. coli for the highest versus the lowest third of the factor 1 score was 3.16 (1.38 to 7.24; P for trend = 0.006). This stool pattern exhibited a significant positive correlation with fecal isobutyrate, isovalerate, valerate, and hexanoate but showed a significant negative correlation with plasma eicosenoic acid and α-linoleic acid, as well as fecal propionate and succinate. No other stool patterns were significant. CONCLUSIONS: These results suggest that stool patterns may be useful in the evaluation of the presence of tumorigenic bacteria and fecal fatty acids through self-monitoring of stool status without the requirement for specialist technology or skill. Furthermore, it may provide valuable insight about effective strategies for the early discovery of CRC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02255-6.
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spelling pubmed-82403562021-06-30 Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults Watanabe, Daiki Murakami, Haruka Ohno, Harumi Tanisawa, Kumpei Konishi, Kana Todoroki-Mori, Kikue Tsunematsu, Yuta Sato, Michio Ogata, Yuji Miyoshi, Noriyuki Kubota, Naoto Kunisawa, Jun Wakabayashi, Keiji Kubota, Tetsuya Watanabe, Kenji Miyachi, Motohiko BMC Microbiol Research Article BACKGROUND: Colibactin-producing Escherichia coli containing polyketide synthase (pks(+) E. coli) has been shown to be involved in colorectal cancer (CRC) development through gut microbiota analysis in animal models. Stool status has been associated with potentially adverse gut microbiome profiles from fecal analysis in adults. We examined the association between stool patterns and the prevalence of pks(+) E. coli isolated from microbiota in fecal samples of 224 healthy Japanese individuals. RESULTS: Stool patterns were determined through factorial analysis using a previously validated questionnaire that included stool frequency, volume, color, shape, and odor. Factor scores were classified into tertiles. The prevalence of pks(+) E. coli was determined by using specific primers for pks(+) E. coli in fecal samples. Plasma and fecal fatty acids were measured via gas chromatography-mass spectrometry. The prevalence of pks(+) E. coli was 26.8%. Three stool patterns identified by factorial analysis accounted for 70.1% of all patterns seen (factor 1: lower frequency, darker color, and harder shape; factor 2: higher volume and softer shape; and factor 3: darker color and stronger odor). Multivariable-adjusted odds ratios (95% confidence intervals) of the prevalence of pks(+) E. coli for the highest versus the lowest third of the factor 1 score was 3.16 (1.38 to 7.24; P for trend = 0.006). This stool pattern exhibited a significant positive correlation with fecal isobutyrate, isovalerate, valerate, and hexanoate but showed a significant negative correlation with plasma eicosenoic acid and α-linoleic acid, as well as fecal propionate and succinate. No other stool patterns were significant. CONCLUSIONS: These results suggest that stool patterns may be useful in the evaluation of the presence of tumorigenic bacteria and fecal fatty acids through self-monitoring of stool status without the requirement for specialist technology or skill. Furthermore, it may provide valuable insight about effective strategies for the early discovery of CRC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-021-02255-6. BioMed Central 2021-06-28 /pmc/articles/PMC8240356/ /pubmed/34182940 http://dx.doi.org/10.1186/s12866-021-02255-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Watanabe, Daiki
Murakami, Haruka
Ohno, Harumi
Tanisawa, Kumpei
Konishi, Kana
Todoroki-Mori, Kikue
Tsunematsu, Yuta
Sato, Michio
Ogata, Yuji
Miyoshi, Noriyuki
Kubota, Naoto
Kunisawa, Jun
Wakabayashi, Keiji
Kubota, Tetsuya
Watanabe, Kenji
Miyachi, Motohiko
Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults
title Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults
title_full Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults
title_fullStr Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults
title_full_unstemmed Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults
title_short Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults
title_sort stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy japanese adults
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240356/
https://www.ncbi.nlm.nih.gov/pubmed/34182940
http://dx.doi.org/10.1186/s12866-021-02255-6
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