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Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood

AIM: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. MATERIALS &...

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Autores principales: Wang, Dezhen, Xu, Peining, Mesaros, Clementina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Newlands Press Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240607/
https://www.ncbi.nlm.nih.gov/pubmed/34110924
http://dx.doi.org/10.4155/bio-2021-0098
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author Wang, Dezhen
Xu, Peining
Mesaros, Clementina
author_facet Wang, Dezhen
Xu, Peining
Mesaros, Clementina
author_sort Wang, Dezhen
collection PubMed
description AIM: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. MATERIALS & METHODS: In this study, we provide a comprehensive method for quantification of the whole blood (WB) sphingolipidome, combining a single-phase extraction method with LC–high-resolution mass spectrometry. RESULTS: We were able to quantify more than 150 sphingolipids, and when compared with paired plasma, WB contained higher concentration of most sphingolipids and individual variations were lower. These findings suggest that WB could be a better alternative to plasma, and potentially guide the evaluation of the sphingolipidome for biomarker discovery.
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spelling pubmed-82406072021-06-29 Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood Wang, Dezhen Xu, Peining Mesaros, Clementina Bioanalysis Methodology AIM: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. MATERIALS & METHODS: In this study, we provide a comprehensive method for quantification of the whole blood (WB) sphingolipidome, combining a single-phase extraction method with LC–high-resolution mass spectrometry. RESULTS: We were able to quantify more than 150 sphingolipids, and when compared with paired plasma, WB contained higher concentration of most sphingolipids and individual variations were lower. These findings suggest that WB could be a better alternative to plasma, and potentially guide the evaluation of the sphingolipidome for biomarker discovery. Newlands Press Ltd 2021-06-10 2021-07 /pmc/articles/PMC8240607/ /pubmed/34110924 http://dx.doi.org/10.4155/bio-2021-0098 Text en © 2021 Wang, Xu & Mesaros https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Methodology
Wang, Dezhen
Xu, Peining
Mesaros, Clementina
Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
title Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
title_full Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
title_fullStr Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
title_full_unstemmed Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
title_short Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
title_sort analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240607/
https://www.ncbi.nlm.nih.gov/pubmed/34110924
http://dx.doi.org/10.4155/bio-2021-0098
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