Cargando…
Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
AIM: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. MATERIALS &...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Newlands Press Ltd
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240607/ https://www.ncbi.nlm.nih.gov/pubmed/34110924 http://dx.doi.org/10.4155/bio-2021-0098 |
_version_ | 1783715245356744704 |
---|---|
author | Wang, Dezhen Xu, Peining Mesaros, Clementina |
author_facet | Wang, Dezhen Xu, Peining Mesaros, Clementina |
author_sort | Wang, Dezhen |
collection | PubMed |
description | AIM: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. MATERIALS & METHODS: In this study, we provide a comprehensive method for quantification of the whole blood (WB) sphingolipidome, combining a single-phase extraction method with LC–high-resolution mass spectrometry. RESULTS: We were able to quantify more than 150 sphingolipids, and when compared with paired plasma, WB contained higher concentration of most sphingolipids and individual variations were lower. These findings suggest that WB could be a better alternative to plasma, and potentially guide the evaluation of the sphingolipidome for biomarker discovery. |
format | Online Article Text |
id | pubmed-8240607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Newlands Press Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-82406072021-06-29 Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood Wang, Dezhen Xu, Peining Mesaros, Clementina Bioanalysis Methodology AIM: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. MATERIALS & METHODS: In this study, we provide a comprehensive method for quantification of the whole blood (WB) sphingolipidome, combining a single-phase extraction method with LC–high-resolution mass spectrometry. RESULTS: We were able to quantify more than 150 sphingolipids, and when compared with paired plasma, WB contained higher concentration of most sphingolipids and individual variations were lower. These findings suggest that WB could be a better alternative to plasma, and potentially guide the evaluation of the sphingolipidome for biomarker discovery. Newlands Press Ltd 2021-06-10 2021-07 /pmc/articles/PMC8240607/ /pubmed/34110924 http://dx.doi.org/10.4155/bio-2021-0098 Text en © 2021 Wang, Xu & Mesaros https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Methodology Wang, Dezhen Xu, Peining Mesaros, Clementina Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood |
title | Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood |
title_full | Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood |
title_fullStr | Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood |
title_full_unstemmed | Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood |
title_short | Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood |
title_sort | analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240607/ https://www.ncbi.nlm.nih.gov/pubmed/34110924 http://dx.doi.org/10.4155/bio-2021-0098 |
work_keys_str_mv | AT wangdezhen analyticalconsiderationsforreducingthematrixeffectforthesphingolipidomequantificationinwholeblood AT xupeining analyticalconsiderationsforreducingthematrixeffectforthesphingolipidomequantificationinwholeblood AT mesarosclementina analyticalconsiderationsforreducingthematrixeffectforthesphingolipidomequantificationinwholeblood |