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Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro

Sheeppox (SPP) is a highly contagious disease of small ruminants caused by sheeppox virus (SPPV) and predominantly occurs in Asia and Africa with significant economic losses. SPPV is genetically and immunologically closely related to goatpox virus (GTPV) and lumpy skin disease virus (LSDV), which in...

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Autores principales: Chibssa, Tesfaye Rufael, Kangethe, Richard Thiga, Berguido, Francisco J., Settypalli, Tirumala Bharani K., Liu, Yang, Grabherr, Reingard, Loitsch, Angelika, Sassu, Elena Lucia, Pichler, Rudolf, Cattoli, Giovanni, Diallo, Adama, Wijewardana, Viskam, Lamien, Charles Euloge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240667/
https://www.ncbi.nlm.nih.gov/pubmed/34211465
http://dx.doi.org/10.3389/fimmu.2021.666543
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author Chibssa, Tesfaye Rufael
Kangethe, Richard Thiga
Berguido, Francisco J.
Settypalli, Tirumala Bharani K.
Liu, Yang
Grabherr, Reingard
Loitsch, Angelika
Sassu, Elena Lucia
Pichler, Rudolf
Cattoli, Giovanni
Diallo, Adama
Wijewardana, Viskam
Lamien, Charles Euloge
author_facet Chibssa, Tesfaye Rufael
Kangethe, Richard Thiga
Berguido, Francisco J.
Settypalli, Tirumala Bharani K.
Liu, Yang
Grabherr, Reingard
Loitsch, Angelika
Sassu, Elena Lucia
Pichler, Rudolf
Cattoli, Giovanni
Diallo, Adama
Wijewardana, Viskam
Lamien, Charles Euloge
author_sort Chibssa, Tesfaye Rufael
collection PubMed
description Sheeppox (SPP) is a highly contagious disease of small ruminants caused by sheeppox virus (SPPV) and predominantly occurs in Asia and Africa with significant economic losses. SPPV is genetically and immunologically closely related to goatpox virus (GTPV) and lumpy skin disease virus (LSDV), which infect goats and cattle respectively. SPPV live attenuated vaccines (LAVs) are used for vaccination against SPP and goatpox (GTP). Mechanisms related to innate immunity elicited by SPPV are unknown. Although adaptive immunity is responsible for long-term immunity, it is the innate responses that prevent viral invasion and replication before LAVs generate specific long-term protection. We analyzed the relative expression of thirteen selected genes that included pattern recognition receptors (PRRs), Nuclear factor-κβ p65 (NF-κβ), and cytokines to understand better the interaction between SPPV and its host. The transcripts of targeted genes in sheep PBMC incubated with either wild type (WT) or LAV SPPV were analyzed using quantitative PCR. Among PRRs, we observed a significantly higher expression of RIG-1 in PBMC incubated with both WT and LAV, with the former producing the highest expression level. However, there was high inter-individual variability in cytokine transcripts levels among different donors, with the expression of TNFα, IL-15, and IL-10 all significantly higher in both PBMC infected with either WT or LAV compared to control PBMC. Correlation studies revealed a strong significant correlation between RIG-1 and IL-10, between TLR4, TNFα, and NF-κβ, between IL-18 and IL-15, and between NF-κβ and IL-10. There was also a significant negative correlation between RIG-1 and IFNγ, between TLR3 and IL-1 β, and between TLR4 and IL-15 (P< 0.05). This study identified RIG-1 as an important PRR in the signaling pathway of innate immune activation during SPPV infection, possibly through intermediate viral dsRNA. The role of immunomodulatory molecules produced by SPPV capable of inhibiting downstream signaling activation following RIG-1 upregulation is discussed. These findings advance our knowledge of the induction of immune responses by SPPV and will help develop safer and more potent vaccines against SPP and GTP.
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spelling pubmed-82406672021-06-30 Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro Chibssa, Tesfaye Rufael Kangethe, Richard Thiga Berguido, Francisco J. Settypalli, Tirumala Bharani K. Liu, Yang Grabherr, Reingard Loitsch, Angelika Sassu, Elena Lucia Pichler, Rudolf Cattoli, Giovanni Diallo, Adama Wijewardana, Viskam Lamien, Charles Euloge Front Immunol Immunology Sheeppox (SPP) is a highly contagious disease of small ruminants caused by sheeppox virus (SPPV) and predominantly occurs in Asia and Africa with significant economic losses. SPPV is genetically and immunologically closely related to goatpox virus (GTPV) and lumpy skin disease virus (LSDV), which infect goats and cattle respectively. SPPV live attenuated vaccines (LAVs) are used for vaccination against SPP and goatpox (GTP). Mechanisms related to innate immunity elicited by SPPV are unknown. Although adaptive immunity is responsible for long-term immunity, it is the innate responses that prevent viral invasion and replication before LAVs generate specific long-term protection. We analyzed the relative expression of thirteen selected genes that included pattern recognition receptors (PRRs), Nuclear factor-κβ p65 (NF-κβ), and cytokines to understand better the interaction between SPPV and its host. The transcripts of targeted genes in sheep PBMC incubated with either wild type (WT) or LAV SPPV were analyzed using quantitative PCR. Among PRRs, we observed a significantly higher expression of RIG-1 in PBMC incubated with both WT and LAV, with the former producing the highest expression level. However, there was high inter-individual variability in cytokine transcripts levels among different donors, with the expression of TNFα, IL-15, and IL-10 all significantly higher in both PBMC infected with either WT or LAV compared to control PBMC. Correlation studies revealed a strong significant correlation between RIG-1 and IL-10, between TLR4, TNFα, and NF-κβ, between IL-18 and IL-15, and between NF-κβ and IL-10. There was also a significant negative correlation between RIG-1 and IFNγ, between TLR3 and IL-1 β, and between TLR4 and IL-15 (P< 0.05). This study identified RIG-1 as an important PRR in the signaling pathway of innate immune activation during SPPV infection, possibly through intermediate viral dsRNA. The role of immunomodulatory molecules produced by SPPV capable of inhibiting downstream signaling activation following RIG-1 upregulation is discussed. These findings advance our knowledge of the induction of immune responses by SPPV and will help develop safer and more potent vaccines against SPP and GTP. Frontiers Media S.A. 2021-06-15 /pmc/articles/PMC8240667/ /pubmed/34211465 http://dx.doi.org/10.3389/fimmu.2021.666543 Text en Copyright © 2021 Chibssa, Kangethe, Berguido, Settypalli, Liu, Grabherr, Loitsch, Sassu, Pichler, Cattoli, Diallo, Wijewardana and Lamien https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Chibssa, Tesfaye Rufael
Kangethe, Richard Thiga
Berguido, Francisco J.
Settypalli, Tirumala Bharani K.
Liu, Yang
Grabherr, Reingard
Loitsch, Angelika
Sassu, Elena Lucia
Pichler, Rudolf
Cattoli, Giovanni
Diallo, Adama
Wijewardana, Viskam
Lamien, Charles Euloge
Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro
title Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro
title_full Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro
title_fullStr Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro
title_full_unstemmed Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro
title_short Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro
title_sort innate immune responses to wildtype and attenuated sheeppox virus mediated through rig-1 sensing in pbmc in-vitro
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240667/
https://www.ncbi.nlm.nih.gov/pubmed/34211465
http://dx.doi.org/10.3389/fimmu.2021.666543
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