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Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure

Despite all modern advances in medicine, there are few reports of effective and safe drugs to treat obesity. Our objective was to screen anti-obesity natural compounds, and to verify whether they can reduce the body weight gain and investigate their molecular mechanisms. By using drug-screening meth...

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Autores principales: Zhang, Yunxia, Li, Jin, Wang, Hui-hui, Li, Jiao, Yu, Yue, Li, Bo, Huang, Li, Wu, Changjing, Liu, Xiaomeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240727/
https://www.ncbi.nlm.nih.gov/pubmed/33983894
http://dx.doi.org/10.1530/JME-20-0349
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author Zhang, Yunxia
Li, Jin
Wang, Hui-hui
Li, Jiao
Yu, Yue
Li, Bo
Huang, Li
Wu, Changjing
Liu, Xiaomeng
author_facet Zhang, Yunxia
Li, Jin
Wang, Hui-hui
Li, Jiao
Yu, Yue
Li, Bo
Huang, Li
Wu, Changjing
Liu, Xiaomeng
author_sort Zhang, Yunxia
collection PubMed
description Despite all modern advances in medicine, there are few reports of effective and safe drugs to treat obesity. Our objective was to screen anti-obesity natural compounds, and to verify whether they can reduce the body weight gain and investigate their molecular mechanisms. By using drug-screening methods, Phytohemagglutinin (PHA) was found to be the most anti-obesity candidate natural compound. Six-week-old C57BL/6J mice were fed with a high-fat diet (HFD) and intraperitoneally injected with 0.25 mg/kg PHA everyday for 8 weeks. The body weight, glucose homeostasis, oxygen consumption and physical activity were assessed. We also measured the heat intensity, body temperature and the gene expression of key regulators of energy expenditure. Prevention study results showed PHA treatment not only reduced the body weight gain but also maintained glucose homeostasis in HFD-fed mice. Further study indicated energy expenditure and uncoupling protein 1 (UCP-1) expression of brown adipose tissue (BAT) and white adipose tissue (WAT) in HFD-fed mice were significantly improved by PHA. In the therapeutic study, a similar effect was observed. PHA inhibited lipid droplet formation and upregulated mitochondrial-related gene expression during adipogenesis in vitro. UCP-1 KO mice displayed no differences in body weight, glucose homeostasis and core body temperature between PHA and control groups. Our results suggest that PHA prevent and treat obesity by increasing energy expenditure through upregulation of BAT thermogenesis.
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spelling pubmed-82407272021-07-01 Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure Zhang, Yunxia Li, Jin Wang, Hui-hui Li, Jiao Yu, Yue Li, Bo Huang, Li Wu, Changjing Liu, Xiaomeng J Mol Endocrinol Research Despite all modern advances in medicine, there are few reports of effective and safe drugs to treat obesity. Our objective was to screen anti-obesity natural compounds, and to verify whether they can reduce the body weight gain and investigate their molecular mechanisms. By using drug-screening methods, Phytohemagglutinin (PHA) was found to be the most anti-obesity candidate natural compound. Six-week-old C57BL/6J mice were fed with a high-fat diet (HFD) and intraperitoneally injected with 0.25 mg/kg PHA everyday for 8 weeks. The body weight, glucose homeostasis, oxygen consumption and physical activity were assessed. We also measured the heat intensity, body temperature and the gene expression of key regulators of energy expenditure. Prevention study results showed PHA treatment not only reduced the body weight gain but also maintained glucose homeostasis in HFD-fed mice. Further study indicated energy expenditure and uncoupling protein 1 (UCP-1) expression of brown adipose tissue (BAT) and white adipose tissue (WAT) in HFD-fed mice were significantly improved by PHA. In the therapeutic study, a similar effect was observed. PHA inhibited lipid droplet formation and upregulated mitochondrial-related gene expression during adipogenesis in vitro. UCP-1 KO mice displayed no differences in body weight, glucose homeostasis and core body temperature between PHA and control groups. Our results suggest that PHA prevent and treat obesity by increasing energy expenditure through upregulation of BAT thermogenesis. Bioscientifica Ltd 2021-05-13 /pmc/articles/PMC8240727/ /pubmed/33983894 http://dx.doi.org/10.1530/JME-20-0349 Text en © The authors https://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Zhang, Yunxia
Li, Jin
Wang, Hui-hui
Li, Jiao
Yu, Yue
Li, Bo
Huang, Li
Wu, Changjing
Liu, Xiaomeng
Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure
title Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure
title_full Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure
title_fullStr Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure
title_full_unstemmed Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure
title_short Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure
title_sort phytohemagglutinin ameliorates hfd-induced obesity by increasing energy expenditure
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240727/
https://www.ncbi.nlm.nih.gov/pubmed/33983894
http://dx.doi.org/10.1530/JME-20-0349
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