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Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis
Endosomal sorting complexes required for transport (ESCRT-0, -I, -II, -III) execute cargo sorting and intralumenal vesicle (ILV) formation during conversion of endosomes to multivesicular bodies (MVBs). The AAA-ATPase Vps4 regulates the ESCRT-III polymer to facilitate membrane remodeling and ILV sci...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240856/ https://www.ncbi.nlm.nih.gov/pubmed/34160559 http://dx.doi.org/10.1083/jcb.202102070 |
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author | Tseng, Chun-Che Dean, Shirley Davies, Brian A. Azmi, Ishara F. Pashkova, Natalya Payne, Johanna A. Staffenhagen, Jennifer West, Matt Piper, Robert C. Odorizzi, Greg Katzmann, David J. |
author_facet | Tseng, Chun-Che Dean, Shirley Davies, Brian A. Azmi, Ishara F. Pashkova, Natalya Payne, Johanna A. Staffenhagen, Jennifer West, Matt Piper, Robert C. Odorizzi, Greg Katzmann, David J. |
author_sort | Tseng, Chun-Che |
collection | PubMed |
description | Endosomal sorting complexes required for transport (ESCRT-0, -I, -II, -III) execute cargo sorting and intralumenal vesicle (ILV) formation during conversion of endosomes to multivesicular bodies (MVBs). The AAA-ATPase Vps4 regulates the ESCRT-III polymer to facilitate membrane remodeling and ILV scission during MVB biogenesis. Here, we show that the conserved V domain of ESCRT-associated protein Bro1 (the yeast homologue of mammalian proteins ALIX and HD-PTP) directly stimulates Vps4. This activity is required for MVB cargo sorting. Furthermore, the Bro1 V domain alone supports Vps4/ESCRT–driven ILV formation in vivo without efficient MVB cargo sorting. These results reveal a novel activity of the V domains of Bro1 homologues in licensing ESCRT-III–dependent ILV formation and suggest a role in coordinating cargo sorting with membrane remodeling during MVB sorting. Moreover, ubiquitin binding enhances V domain stimulation of Vps4 to promote ILV formation via the Bro1–Vps4–ESCRT-III axis, uncovering a novel role for ubiquitin during MVB biogenesis in addition to facilitating cargo recognition. |
format | Online Article Text |
id | pubmed-8240856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-82408562022-02-02 Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis Tseng, Chun-Che Dean, Shirley Davies, Brian A. Azmi, Ishara F. Pashkova, Natalya Payne, Johanna A. Staffenhagen, Jennifer West, Matt Piper, Robert C. Odorizzi, Greg Katzmann, David J. J Cell Biol Article Endosomal sorting complexes required for transport (ESCRT-0, -I, -II, -III) execute cargo sorting and intralumenal vesicle (ILV) formation during conversion of endosomes to multivesicular bodies (MVBs). The AAA-ATPase Vps4 regulates the ESCRT-III polymer to facilitate membrane remodeling and ILV scission during MVB biogenesis. Here, we show that the conserved V domain of ESCRT-associated protein Bro1 (the yeast homologue of mammalian proteins ALIX and HD-PTP) directly stimulates Vps4. This activity is required for MVB cargo sorting. Furthermore, the Bro1 V domain alone supports Vps4/ESCRT–driven ILV formation in vivo without efficient MVB cargo sorting. These results reveal a novel activity of the V domains of Bro1 homologues in licensing ESCRT-III–dependent ILV formation and suggest a role in coordinating cargo sorting with membrane remodeling during MVB sorting. Moreover, ubiquitin binding enhances V domain stimulation of Vps4 to promote ILV formation via the Bro1–Vps4–ESCRT-III axis, uncovering a novel role for ubiquitin during MVB biogenesis in addition to facilitating cargo recognition. Rockefeller University Press 2021-06-23 /pmc/articles/PMC8240856/ /pubmed/34160559 http://dx.doi.org/10.1083/jcb.202102070 Text en © 2021 Tseng et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Tseng, Chun-Che Dean, Shirley Davies, Brian A. Azmi, Ishara F. Pashkova, Natalya Payne, Johanna A. Staffenhagen, Jennifer West, Matt Piper, Robert C. Odorizzi, Greg Katzmann, David J. Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis |
title | Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis |
title_full | Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis |
title_fullStr | Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis |
title_full_unstemmed | Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis |
title_short | Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis |
title_sort | bro1 stimulates vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240856/ https://www.ncbi.nlm.nih.gov/pubmed/34160559 http://dx.doi.org/10.1083/jcb.202102070 |
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