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Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions
Significance: Noninvasive diffuse optical spectroscopy (DOS) is a promising adjunct diagnostic imaging technique for distinguishing benign and malignant breast lesions. Most DOS approaches require normalizing lesion biomarkers to healthy tissue since major tissue constituents exhibit large interpati...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240868/ https://www.ncbi.nlm.nih.gov/pubmed/34189876 http://dx.doi.org/10.1117/1.JBO.26.6.065004 |
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author | Vasudevan, Sandhya Campbell, Chris Liu, Fang O’Sullivan, Thomas D. |
author_facet | Vasudevan, Sandhya Campbell, Chris Liu, Fang O’Sullivan, Thomas D. |
author_sort | Vasudevan, Sandhya |
collection | PubMed |
description | Significance: Noninvasive diffuse optical spectroscopy (DOS) is a promising adjunct diagnostic imaging technique for distinguishing benign and malignant breast lesions. Most DOS approaches require normalizing lesion biomarkers to healthy tissue since major tissue constituents exhibit large interpatient variations. However, absolute optical biomarkers are desirable as it avoids reference measurements which may be difficult or impractical to acquire. Aim: Our goal is to determine whether absolute measurements of minor absorbers such as collagen and methemoglobin (metHb) can successfully distinguish lesions. We hypothesize that metHb would exhibit less interpatient variability and be more suitable as an absolute metric for malignancy. However, we would expect collagen to exhibit more variability, because unlike metHb, collagen is also present in the healthy tissue. Approach: In this retrospective clinical study, 30 lesions with breast imaging reporting and database system score [Formula: see text] (12 benign and 18 malignant) measured with broadband quantitative DOS were analyzed for their oxyhemoglobin (HbO), deoxyhemoglobin (HHb), water, lipids, collagen, metHb concentrations, and optical scattering characteristics. Wilcoxon rank sum test was used to compare benign and malignant lesions for all variables in both normalized and absolute forms. Results: Among all absolute DOS parameters considered, only absolute metHb was observed to be significant for lesion discrimination ([Formula: see text] for benign versus [Formula: see text] for malignant, [Formula: see text]). Absolute metHb concentration was also determined to be the best predictor of malignancy with an area under the curve of 0.89. Conclusions: Our findings demonstrate that lesion metHb concentration measured by DOS can improve noninvasive optical diagnosis of breast malignancies. Since metHb concentration found in normal breast tissue is extremely low, metHb may be a more direct indicator of malignancy that does not depend on other biomarkers found in healthy tissue with significant variability. Furthermore, absolute parameters require reduced measurement time and can be utilized in cases where healthy reference tissue is not available. |
format | Online Article Text |
id | pubmed-8240868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-82408682021-06-30 Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions Vasudevan, Sandhya Campbell, Chris Liu, Fang O’Sullivan, Thomas D. J Biomed Opt General Significance: Noninvasive diffuse optical spectroscopy (DOS) is a promising adjunct diagnostic imaging technique for distinguishing benign and malignant breast lesions. Most DOS approaches require normalizing lesion biomarkers to healthy tissue since major tissue constituents exhibit large interpatient variations. However, absolute optical biomarkers are desirable as it avoids reference measurements which may be difficult or impractical to acquire. Aim: Our goal is to determine whether absolute measurements of minor absorbers such as collagen and methemoglobin (metHb) can successfully distinguish lesions. We hypothesize that metHb would exhibit less interpatient variability and be more suitable as an absolute metric for malignancy. However, we would expect collagen to exhibit more variability, because unlike metHb, collagen is also present in the healthy tissue. Approach: In this retrospective clinical study, 30 lesions with breast imaging reporting and database system score [Formula: see text] (12 benign and 18 malignant) measured with broadband quantitative DOS were analyzed for their oxyhemoglobin (HbO), deoxyhemoglobin (HHb), water, lipids, collagen, metHb concentrations, and optical scattering characteristics. Wilcoxon rank sum test was used to compare benign and malignant lesions for all variables in both normalized and absolute forms. Results: Among all absolute DOS parameters considered, only absolute metHb was observed to be significant for lesion discrimination ([Formula: see text] for benign versus [Formula: see text] for malignant, [Formula: see text]). Absolute metHb concentration was also determined to be the best predictor of malignancy with an area under the curve of 0.89. Conclusions: Our findings demonstrate that lesion metHb concentration measured by DOS can improve noninvasive optical diagnosis of breast malignancies. Since metHb concentration found in normal breast tissue is extremely low, metHb may be a more direct indicator of malignancy that does not depend on other biomarkers found in healthy tissue with significant variability. Furthermore, absolute parameters require reduced measurement time and can be utilized in cases where healthy reference tissue is not available. Society of Photo-Optical Instrumentation Engineers 2021-06-29 2021-06 /pmc/articles/PMC8240868/ /pubmed/34189876 http://dx.doi.org/10.1117/1.JBO.26.6.065004 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | General Vasudevan, Sandhya Campbell, Chris Liu, Fang O’Sullivan, Thomas D. Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions |
title | Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions |
title_full | Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions |
title_fullStr | Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions |
title_full_unstemmed | Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions |
title_short | Broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions |
title_sort | broadband diffuse optical spectroscopy of absolute methemoglobin concentration can distinguish benign and malignant breast lesions |
topic | General |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240868/ https://www.ncbi.nlm.nih.gov/pubmed/34189876 http://dx.doi.org/10.1117/1.JBO.26.6.065004 |
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