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Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair

Peripheral nerve injuries, associated with significant morbidity, can benefit from electrical stimulation (ES), as demonstrated in animal studies through improved axonal growth. This study combined the clinical gold standard of isograft repair in a rat model of sciatic nerve injury to evaluate the e...

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Autores principales: Koh, Galina P., Fouad, Carol, Lanzinger, William, Willits, Rebecca Kuntz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240900/
https://www.ncbi.nlm.nih.gov/pubmed/34223540
http://dx.doi.org/10.1089/neur.2020.0049
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author Koh, Galina P.
Fouad, Carol
Lanzinger, William
Willits, Rebecca Kuntz
author_facet Koh, Galina P.
Fouad, Carol
Lanzinger, William
Willits, Rebecca Kuntz
author_sort Koh, Galina P.
collection PubMed
description Peripheral nerve injuries, associated with significant morbidity, can benefit from electrical stimulation (ES), as demonstrated in animal studies through improved axonal growth. This study combined the clinical gold standard of isograft repair in a rat model of sciatic nerve injury to evaluate the effects of intraoperative ES on functional tests and histology. Forty rats underwent a surgically induced gap injury to the right sciatic nerve and subsequent repair with an isograft. Half of these rats were randomly selected to receive 10 min of intraoperative ES. Functional testing, including response time to a heat stimulus and motor functional tests, were conducted. Histology of the sciatic nerves and gastrocnemius muscles were analyzed after 6 and 12 weeks of recovery. Rats that underwent ES treatment showed incremental improvements in motor function between weeks 2 and 12, with a significantly higher push-off response than the no-ES controls after 6 weeks. Although no differences were detected between groups in the sensory testing, significant improvements over time were noted in the ES group. Histology parameters, sciatic nerve measures, and gastrocnemius muscle weights demonstrated nerve recovery over time for both the ES and no-ES control groups. Although ES promoted improvements in motor function comparable to that in previous studies, the benefits of intraoperative ES were not detectable in other metrics of this rat model of peripheral nerve injury. Future work is needed to optimize sensory testing in the rodent injury model and compare electrical activity of collagen scaffolds to native tissue to detect differences.
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spelling pubmed-82409002021-07-02 Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair Koh, Galina P. Fouad, Carol Lanzinger, William Willits, Rebecca Kuntz Neurotrauma Rep Original Article Peripheral nerve injuries, associated with significant morbidity, can benefit from electrical stimulation (ES), as demonstrated in animal studies through improved axonal growth. This study combined the clinical gold standard of isograft repair in a rat model of sciatic nerve injury to evaluate the effects of intraoperative ES on functional tests and histology. Forty rats underwent a surgically induced gap injury to the right sciatic nerve and subsequent repair with an isograft. Half of these rats were randomly selected to receive 10 min of intraoperative ES. Functional testing, including response time to a heat stimulus and motor functional tests, were conducted. Histology of the sciatic nerves and gastrocnemius muscles were analyzed after 6 and 12 weeks of recovery. Rats that underwent ES treatment showed incremental improvements in motor function between weeks 2 and 12, with a significantly higher push-off response than the no-ES controls after 6 weeks. Although no differences were detected between groups in the sensory testing, significant improvements over time were noted in the ES group. Histology parameters, sciatic nerve measures, and gastrocnemius muscle weights demonstrated nerve recovery over time for both the ES and no-ES control groups. Although ES promoted improvements in motor function comparable to that in previous studies, the benefits of intraoperative ES were not detectable in other metrics of this rat model of peripheral nerve injury. Future work is needed to optimize sensory testing in the rodent injury model and compare electrical activity of collagen scaffolds to native tissue to detect differences. Mary Ann Liebert, Inc., publishers 2020-11-12 /pmc/articles/PMC8240900/ /pubmed/34223540 http://dx.doi.org/10.1089/neur.2020.0049 Text en © Galina P. Koh et al., 2020; Published by Mary Ann Liebert, Inc. https://creativecommons.org/licenses/by/4.0/This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Original Article
Koh, Galina P.
Fouad, Carol
Lanzinger, William
Willits, Rebecca Kuntz
Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair
title Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair
title_full Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair
title_fullStr Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair
title_full_unstemmed Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair
title_short Effect of Intraoperative Electrical Stimulation on Recovery after Rat Sciatic Nerve Isograft Repair
title_sort effect of intraoperative electrical stimulation on recovery after rat sciatic nerve isograft repair
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240900/
https://www.ncbi.nlm.nih.gov/pubmed/34223540
http://dx.doi.org/10.1089/neur.2020.0049
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