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Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor

Docetaxel (DTX) is found to be very effective against glioma cell in vitro. However, in vivo passage of DTX through BBB is extremely difficult due to the physicochemical and pharmacological characteristics of the drug. No existing formulation is successful in this aspect. Hence, in this study, effor...

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Autores principales: Shaw, Tapan Kumar, Mandal, Dipika, Dey, Goutam, Pal, Murari Mohan, Paul, Paramita, Chakraborty, Samrat, Ali, Kazi Asraf, Mukherjee, Biswajit, Bandyopadhyay, Amal Kumar, Mandal, Mahitosh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240984/
https://www.ncbi.nlm.nih.gov/pubmed/28165821
http://dx.doi.org/10.1080/10717544.2016.1253798
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author Shaw, Tapan Kumar
Mandal, Dipika
Dey, Goutam
Pal, Murari Mohan
Paul, Paramita
Chakraborty, Samrat
Ali, Kazi Asraf
Mukherjee, Biswajit
Bandyopadhyay, Amal Kumar
Mandal, Mahitosh
author_facet Shaw, Tapan Kumar
Mandal, Dipika
Dey, Goutam
Pal, Murari Mohan
Paul, Paramita
Chakraborty, Samrat
Ali, Kazi Asraf
Mukherjee, Biswajit
Bandyopadhyay, Amal Kumar
Mandal, Mahitosh
author_sort Shaw, Tapan Kumar
collection PubMed
description Docetaxel (DTX) is found to be very effective against glioma cell in vitro. However, in vivo passage of DTX through BBB is extremely difficult due to the physicochemical and pharmacological characteristics of the drug. No existing formulation is successful in this aspect. Hence, in this study, effort was made to send DTX through blood–brain barrier (BBB) to brain to treat diseases such as solid tumor of brain (glioma) by developing DTX-loaded nanoliposomes. Primarily drug-excipients interaction was evaluated by FTIR spectroscopy. The DTX-loaded nanoliposomes (L-DTX) were prepared by lipid layer hydration technique and characterized physicochemically. In vitro cellular uptake in C6 glioma cells was investigated. FTIR data show that the selected drug and excipients were chemically compatible. The unilamellar vesicle size was less than 50 nm with smooth surface. Drug released slowly from L-DTX in vitro in a sustained manner. The pharmacokinetic data shows more extended action of DTX from L-DTX in experimental rats than the free-drug and Taxotere®. DTX from L-DTX enhanced 100% drug concentration in brain as compared with Taxotere® in 4 h. Thus, nanoliposomes as vehicle may be an encouraging strategy to treat glioma with DTX.
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spelling pubmed-82409842021-07-08 Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor Shaw, Tapan Kumar Mandal, Dipika Dey, Goutam Pal, Murari Mohan Paul, Paramita Chakraborty, Samrat Ali, Kazi Asraf Mukherjee, Biswajit Bandyopadhyay, Amal Kumar Mandal, Mahitosh Drug Deliv Research Article Docetaxel (DTX) is found to be very effective against glioma cell in vitro. However, in vivo passage of DTX through BBB is extremely difficult due to the physicochemical and pharmacological characteristics of the drug. No existing formulation is successful in this aspect. Hence, in this study, effort was made to send DTX through blood–brain barrier (BBB) to brain to treat diseases such as solid tumor of brain (glioma) by developing DTX-loaded nanoliposomes. Primarily drug-excipients interaction was evaluated by FTIR spectroscopy. The DTX-loaded nanoliposomes (L-DTX) were prepared by lipid layer hydration technique and characterized physicochemically. In vitro cellular uptake in C6 glioma cells was investigated. FTIR data show that the selected drug and excipients were chemically compatible. The unilamellar vesicle size was less than 50 nm with smooth surface. Drug released slowly from L-DTX in vitro in a sustained manner. The pharmacokinetic data shows more extended action of DTX from L-DTX in experimental rats than the free-drug and Taxotere®. DTX from L-DTX enhanced 100% drug concentration in brain as compared with Taxotere® in 4 h. Thus, nanoliposomes as vehicle may be an encouraging strategy to treat glioma with DTX. Taylor & Francis 2017-02-06 /pmc/articles/PMC8240984/ /pubmed/28165821 http://dx.doi.org/10.1080/10717544.2016.1253798 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shaw, Tapan Kumar
Mandal, Dipika
Dey, Goutam
Pal, Murari Mohan
Paul, Paramita
Chakraborty, Samrat
Ali, Kazi Asraf
Mukherjee, Biswajit
Bandyopadhyay, Amal Kumar
Mandal, Mahitosh
Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor
title Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor
title_full Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor
title_fullStr Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor
title_full_unstemmed Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor
title_short Successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor
title_sort successful delivery of docetaxel to rat brain using experimentally developed nanoliposome: a treatment strategy for brain tumor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240984/
https://www.ncbi.nlm.nih.gov/pubmed/28165821
http://dx.doi.org/10.1080/10717544.2016.1253798
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