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Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis

Electro-phonophoresis (EP) has been used as a drug delivery approach in clinical fields. The objective of the present study is to evaluate the skin permeability of isoniazid and rifampin in guinea pigs by EP to provide reference basis for clinical applications of such transdermal delivery system in...

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Autores principales: Chen, Suting, Han, Yi, Yu, Daping, Huo, Fengmin, Wang, Fen, Li, Yunxu, Dong, Lingling, Liu, Zhidong, Huang, Hairong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241010/
https://www.ncbi.nlm.nih.gov/pubmed/28181840
http://dx.doi.org/10.1080/10717544.2016.1267275
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author Chen, Suting
Han, Yi
Yu, Daping
Huo, Fengmin
Wang, Fen
Li, Yunxu
Dong, Lingling
Liu, Zhidong
Huang, Hairong
author_facet Chen, Suting
Han, Yi
Yu, Daping
Huo, Fengmin
Wang, Fen
Li, Yunxu
Dong, Lingling
Liu, Zhidong
Huang, Hairong
author_sort Chen, Suting
collection PubMed
description Electro-phonophoresis (EP) has been used as a drug delivery approach in clinical fields. The objective of the present study is to evaluate the skin permeability of isoniazid and rifampin in guinea pigs by EP to provide reference basis for clinical applications of such transdermal delivery system in the treatment of patients with superficial tuberculosis. Isoniazid and rifampin solutions were delivered transdermally with or without EP in health guinea pigs for 0.5 h. Local skin and blood samples were collected serially at 0, 1/2, 1, 2, 4, 6 and 24 h after dosing. Drug concentrations in local skin and blood were evaluated by high-performance liquid chromatography. Isoniazid concentrations in local skin of guinea pigs receiving isoniazid through EP transdermal delivery were significantly higher than in animals receiving only isoniazid with transdermal patch. However, for rifampin, patches alone group presented almost uniform concentration versus time curve with that of EP group, and both groups had concentrations much higher than the therapeutic concentration of the drug over sustainable time. After EP transdermal delivery, the mean peak concentrations of isoniazid and rifampin in skin were 771.0 ± 163.4 μg/mL and 81.2 ± 17.3 μg/mL respectively. Neither isoniazid nor rifampin concentration in blood could be detected (below the lower detection limit of 1 μg/mL) at any time point. The present study showed that application of EP significantly enhanced INH penetration through skin in guinea pigs, while RIF patch alone obtained therapeutic concentration in local skin. Our work suggests several possible medication approaches for efficient treatment of superficial tuberculosis.
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spelling pubmed-82410102021-07-08 Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis Chen, Suting Han, Yi Yu, Daping Huo, Fengmin Wang, Fen Li, Yunxu Dong, Lingling Liu, Zhidong Huang, Hairong Drug Deliv Original Article Electro-phonophoresis (EP) has been used as a drug delivery approach in clinical fields. The objective of the present study is to evaluate the skin permeability of isoniazid and rifampin in guinea pigs by EP to provide reference basis for clinical applications of such transdermal delivery system in the treatment of patients with superficial tuberculosis. Isoniazid and rifampin solutions were delivered transdermally with or without EP in health guinea pigs for 0.5 h. Local skin and blood samples were collected serially at 0, 1/2, 1, 2, 4, 6 and 24 h after dosing. Drug concentrations in local skin and blood were evaluated by high-performance liquid chromatography. Isoniazid concentrations in local skin of guinea pigs receiving isoniazid through EP transdermal delivery were significantly higher than in animals receiving only isoniazid with transdermal patch. However, for rifampin, patches alone group presented almost uniform concentration versus time curve with that of EP group, and both groups had concentrations much higher than the therapeutic concentration of the drug over sustainable time. After EP transdermal delivery, the mean peak concentrations of isoniazid and rifampin in skin were 771.0 ± 163.4 μg/mL and 81.2 ± 17.3 μg/mL respectively. Neither isoniazid nor rifampin concentration in blood could be detected (below the lower detection limit of 1 μg/mL) at any time point. The present study showed that application of EP significantly enhanced INH penetration through skin in guinea pigs, while RIF patch alone obtained therapeutic concentration in local skin. Our work suggests several possible medication approaches for efficient treatment of superficial tuberculosis. Taylor & Francis 2017-02-09 /pmc/articles/PMC8241010/ /pubmed/28181840 http://dx.doi.org/10.1080/10717544.2016.1267275 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chen, Suting
Han, Yi
Yu, Daping
Huo, Fengmin
Wang, Fen
Li, Yunxu
Dong, Lingling
Liu, Zhidong
Huang, Hairong
Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis
title Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis
title_full Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis
title_fullStr Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis
title_full_unstemmed Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis
title_short Transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis
title_sort transdermal delivery of isoniazid and rifampin in guinea pigs by electro-phonophoresis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241010/
https://www.ncbi.nlm.nih.gov/pubmed/28181840
http://dx.doi.org/10.1080/10717544.2016.1267275
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