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Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection
Viral nervous necrosis (VNN) is an acute and serious fish disease caused by nervous necrosis virus (NNV) which has been reported massive mortality in more than fifty teleost species worldwide. VNN causes damage of necrosis and vacuolation to central nervous system (CNS) cells in fish. It is difficul...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241073/ https://www.ncbi.nlm.nih.gov/pubmed/34185811 http://dx.doi.org/10.1371/journal.ppat.1009665 |
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author | Wang, Qing Peng, Cheng Yang, Min Huang, Fengqi Duan, Xuzhuo Wang, Shaowen Cheng, Huitao Yang, Huirong Zhao, Huihong Qin, Qiwei |
author_facet | Wang, Qing Peng, Cheng Yang, Min Huang, Fengqi Duan, Xuzhuo Wang, Shaowen Cheng, Huitao Yang, Huirong Zhao, Huihong Qin, Qiwei |
author_sort | Wang, Qing |
collection | PubMed |
description | Viral nervous necrosis (VNN) is an acute and serious fish disease caused by nervous necrosis virus (NNV) which has been reported massive mortality in more than fifty teleost species worldwide. VNN causes damage of necrosis and vacuolation to central nervous system (CNS) cells in fish. It is difficult to identify the specific type of cell targeted by NNV, and to decipher the host immune response because of the functional diversity and highly complex anatomical and cellular composition of the CNS. In this study, we found that the red spotted grouper NNV (RGNNV) mainly attacked the midbrain of orange-spotted grouper (Epinephelus coioides). We conducted single-cell RNA-seq analysis of the midbrain of healthy and RGNNV-infected fish and identified 35 transcriptionally distinct cell subtypes, including 28 neuronal and 7 non-neuronal cell types. An evaluation of the subpopulations of immune cells revealed that macrophages were enriched in RGNNV-infected fish, and the transcriptional profiles of macrophages indicated an acute cytokine and inflammatory response. Unsupervised pseudotime analysis of immune cells showed that microglia transformed into M1-type activated macrophages to produce cytokines to reduce the damage to nerve tissue caused by the virus. We also found that RGNNV targeted neuronal cell types was GLU1 and GLU3, and we found that the key genes and pathways by which causes cell cytoplasmic vacuoles and autophagy significant enrichment, this may be the major route viruses cause cell death. These data provided a comprehensive transcriptional perspective of the grouper midbrain and the basis for further research on how viruses infect the teleost CNS. |
format | Online Article Text |
id | pubmed-8241073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82410732021-07-09 Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection Wang, Qing Peng, Cheng Yang, Min Huang, Fengqi Duan, Xuzhuo Wang, Shaowen Cheng, Huitao Yang, Huirong Zhao, Huihong Qin, Qiwei PLoS Pathog Research Article Viral nervous necrosis (VNN) is an acute and serious fish disease caused by nervous necrosis virus (NNV) which has been reported massive mortality in more than fifty teleost species worldwide. VNN causes damage of necrosis and vacuolation to central nervous system (CNS) cells in fish. It is difficult to identify the specific type of cell targeted by NNV, and to decipher the host immune response because of the functional diversity and highly complex anatomical and cellular composition of the CNS. In this study, we found that the red spotted grouper NNV (RGNNV) mainly attacked the midbrain of orange-spotted grouper (Epinephelus coioides). We conducted single-cell RNA-seq analysis of the midbrain of healthy and RGNNV-infected fish and identified 35 transcriptionally distinct cell subtypes, including 28 neuronal and 7 non-neuronal cell types. An evaluation of the subpopulations of immune cells revealed that macrophages were enriched in RGNNV-infected fish, and the transcriptional profiles of macrophages indicated an acute cytokine and inflammatory response. Unsupervised pseudotime analysis of immune cells showed that microglia transformed into M1-type activated macrophages to produce cytokines to reduce the damage to nerve tissue caused by the virus. We also found that RGNNV targeted neuronal cell types was GLU1 and GLU3, and we found that the key genes and pathways by which causes cell cytoplasmic vacuoles and autophagy significant enrichment, this may be the major route viruses cause cell death. These data provided a comprehensive transcriptional perspective of the grouper midbrain and the basis for further research on how viruses infect the teleost CNS. Public Library of Science 2021-06-29 /pmc/articles/PMC8241073/ /pubmed/34185811 http://dx.doi.org/10.1371/journal.ppat.1009665 Text en © 2021 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Qing Peng, Cheng Yang, Min Huang, Fengqi Duan, Xuzhuo Wang, Shaowen Cheng, Huitao Yang, Huirong Zhao, Huihong Qin, Qiwei Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection |
title | Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection |
title_full | Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection |
title_fullStr | Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection |
title_full_unstemmed | Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection |
title_short | Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection |
title_sort | single-cell rna-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241073/ https://www.ncbi.nlm.nih.gov/pubmed/34185811 http://dx.doi.org/10.1371/journal.ppat.1009665 |
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