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Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis

During spermatogenesis, nuclear architecture of male germ cells is dynamically changed and epigenetic modifications, in particular methylation of histones, highly contribute to its regulation as well as differentiation of male germ cells. Although several methyltransferases and demethylases for hist...

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Detalles Bibliográficos
Autores principales: Wang, Youtao, Iwamori, Tokuko, Kaneko, Takane, Iida, Hiroshi, Iwamori, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241091/
https://www.ncbi.nlm.nih.gov/pubmed/34185806
http://dx.doi.org/10.1371/journal.pone.0253897
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author Wang, Youtao
Iwamori, Tokuko
Kaneko, Takane
Iida, Hiroshi
Iwamori, Naoki
author_facet Wang, Youtao
Iwamori, Tokuko
Kaneko, Takane
Iida, Hiroshi
Iwamori, Naoki
author_sort Wang, Youtao
collection PubMed
description During spermatogenesis, nuclear architecture of male germ cells is dynamically changed and epigenetic modifications, in particular methylation of histones, highly contribute to its regulation as well as differentiation of male germ cells. Although several methyltransferases and demethylases for histone H3 are involved in the regulation of spermatogenesis, roles of either histone H4 lysine 20 (H4K20) methyltransferases or H4K20 demethylases during spermatogenesis still remain to be elucidated. Recently, RSBN1 which is a testis-specific gene expressed in round spermatids was identified as a demethylase for dimethyl H4K20. In this study, therefore, we confirm the demethylase function of RSBN1 and compare distributions between RSBN1 and methylated H4K20 in the seminiferous tubules. Unlike previous report, expression analyses for RSBN1 reveal that RSBN1 is not a testis-specific gene and is expressed not only in round spermatids but also in elongated spermatids. In addition, RSBN1 can demethylate not only dimethyl H4K20 but also trimethyl H4K20 and could convert both dimethyl H4K20 and trimethyl H4K20 into monomethyl H4K20. When distribution pattern of RSBN1 in the seminiferous tubule is compared to that of methylated H4K20, both dimethyl H4K20 and trimethyl H4K20 but not monomethyl H4K20 are disappeared from RSBN1 positive germ cells, suggesting that testis-specific distribution patterns of methylated H4K20 might be constructed by RSBN1. Thus, novel expression and function of RSBN1 could be useful to comprehend epigenetic regulation during spermatogenesis.
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spelling pubmed-82410912021-07-09 Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis Wang, Youtao Iwamori, Tokuko Kaneko, Takane Iida, Hiroshi Iwamori, Naoki PLoS One Research Article During spermatogenesis, nuclear architecture of male germ cells is dynamically changed and epigenetic modifications, in particular methylation of histones, highly contribute to its regulation as well as differentiation of male germ cells. Although several methyltransferases and demethylases for histone H3 are involved in the regulation of spermatogenesis, roles of either histone H4 lysine 20 (H4K20) methyltransferases or H4K20 demethylases during spermatogenesis still remain to be elucidated. Recently, RSBN1 which is a testis-specific gene expressed in round spermatids was identified as a demethylase for dimethyl H4K20. In this study, therefore, we confirm the demethylase function of RSBN1 and compare distributions between RSBN1 and methylated H4K20 in the seminiferous tubules. Unlike previous report, expression analyses for RSBN1 reveal that RSBN1 is not a testis-specific gene and is expressed not only in round spermatids but also in elongated spermatids. In addition, RSBN1 can demethylate not only dimethyl H4K20 but also trimethyl H4K20 and could convert both dimethyl H4K20 and trimethyl H4K20 into monomethyl H4K20. When distribution pattern of RSBN1 in the seminiferous tubule is compared to that of methylated H4K20, both dimethyl H4K20 and trimethyl H4K20 but not monomethyl H4K20 are disappeared from RSBN1 positive germ cells, suggesting that testis-specific distribution patterns of methylated H4K20 might be constructed by RSBN1. Thus, novel expression and function of RSBN1 could be useful to comprehend epigenetic regulation during spermatogenesis. Public Library of Science 2021-06-29 /pmc/articles/PMC8241091/ /pubmed/34185806 http://dx.doi.org/10.1371/journal.pone.0253897 Text en © 2021 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Youtao
Iwamori, Tokuko
Kaneko, Takane
Iida, Hiroshi
Iwamori, Naoki
Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis
title Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis
title_full Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis
title_fullStr Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis
title_full_unstemmed Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis
title_short Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis
title_sort comparative distributions of rsbn1 and methylated histone h4 lysine 20 in the mouse spermatogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241091/
https://www.ncbi.nlm.nih.gov/pubmed/34185806
http://dx.doi.org/10.1371/journal.pone.0253897
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