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PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations
In this study, PCM and TAT co-modified liposome was developed as a novel drug carrier for myocardium delivery with evaluation of its in vitro and in vivo properties. Liposomes containing fluorescent probe coumarin-6 were prepared by thin-film hydration. The PCM ligands specifically bind to the PCM r...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241121/ https://www.ncbi.nlm.nih.gov/pubmed/28165817 http://dx.doi.org/10.1080/10717544.2016.1253121 |
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author | Wang, Xin Huang, Hua Zhang, Liangke Bai, Yan Chen, Huali |
author_facet | Wang, Xin Huang, Hua Zhang, Liangke Bai, Yan Chen, Huali |
author_sort | Wang, Xin |
collection | PubMed |
description | In this study, PCM and TAT co-modified liposome was developed as a novel drug carrier for myocardium delivery with evaluation of its in vitro and in vivo properties. Liposomes containing fluorescent probe coumarin-6 were prepared by thin-film hydration. The PCM ligands specifically bind to the PCM receptors in the extracellular connective tissue of primary myocardium cells (MCs), while the TAT ligands functioned as a classical cell penetrating peptide to make liposomes internalized by MCs. The unmodified liposome (L), PCM-modified liposome (PL), TAT-modified liposome (TL) and PCM and TAT co-modified liposome (PTL) were prepared and characterized. The cellular uptake and intracellular distribution of various liposomes by MCs demonstrated that PTL had the best delivery capability. Peptide inhibition assay indicated that the uptake of PL could be inhibited by PCM. However, TAT could almost not suppress the uptake of TL. In addition, the CCK-8 experiments showed that liposomes had low cytotoxicity. In vivo fluorescent images of frozen sections and HPLC-fluorescence analysis further demonstrated that PTL had highest myocardium distribution. The results of this study demonstrated that PCM and TAT co-modifying could improve the myocardial targeting ability of liposome. |
format | Online Article Text |
id | pubmed-8241121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-82411212021-07-08 PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations Wang, Xin Huang, Hua Zhang, Liangke Bai, Yan Chen, Huali Drug Deliv Research Article In this study, PCM and TAT co-modified liposome was developed as a novel drug carrier for myocardium delivery with evaluation of its in vitro and in vivo properties. Liposomes containing fluorescent probe coumarin-6 were prepared by thin-film hydration. The PCM ligands specifically bind to the PCM receptors in the extracellular connective tissue of primary myocardium cells (MCs), while the TAT ligands functioned as a classical cell penetrating peptide to make liposomes internalized by MCs. The unmodified liposome (L), PCM-modified liposome (PL), TAT-modified liposome (TL) and PCM and TAT co-modified liposome (PTL) were prepared and characterized. The cellular uptake and intracellular distribution of various liposomes by MCs demonstrated that PTL had the best delivery capability. Peptide inhibition assay indicated that the uptake of PL could be inhibited by PCM. However, TAT could almost not suppress the uptake of TL. In addition, the CCK-8 experiments showed that liposomes had low cytotoxicity. In vivo fluorescent images of frozen sections and HPLC-fluorescence analysis further demonstrated that PTL had highest myocardium distribution. The results of this study demonstrated that PCM and TAT co-modifying could improve the myocardial targeting ability of liposome. Taylor & Francis 2017-02-06 /pmc/articles/PMC8241121/ /pubmed/28165817 http://dx.doi.org/10.1080/10717544.2016.1253121 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Xin Huang, Hua Zhang, Liangke Bai, Yan Chen, Huali PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations |
title | PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations |
title_full | PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations |
title_fullStr | PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations |
title_full_unstemmed | PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations |
title_short | PCM and TAT co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations |
title_sort | pcm and tat co-modified liposome with improved myocardium delivery: in vitro and in vivo evaluations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241121/ https://www.ncbi.nlm.nih.gov/pubmed/28165817 http://dx.doi.org/10.1080/10717544.2016.1253121 |
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