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Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation

In this study, we demonstrate the low toxicity and highly efficient and spatially improved transfection of plasmid DNA (pDNA) with liposomal nanobubbles (bubble liposomes [BLs]) using ultrasound (US) irradiation in mice. Naked pDNA with BLs was intraperitoneally injected, followed by US irradiation....

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Detalles Bibliográficos
Autores principales: Nishimura, Koyo, Fumoto, Shintaro, Fuchigami, Yuki, Hagimori, Masayori, Maruyama, Kazuo, Kawakami, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241157/
https://www.ncbi.nlm.nih.gov/pubmed/28446052
http://dx.doi.org/10.1080/10717544.2017.1319433
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author Nishimura, Koyo
Fumoto, Shintaro
Fuchigami, Yuki
Hagimori, Masayori
Maruyama, Kazuo
Kawakami, Shigeru
author_facet Nishimura, Koyo
Fumoto, Shintaro
Fuchigami, Yuki
Hagimori, Masayori
Maruyama, Kazuo
Kawakami, Shigeru
author_sort Nishimura, Koyo
collection PubMed
description In this study, we demonstrate the low toxicity and highly efficient and spatially improved transfection of plasmid DNA (pDNA) with liposomal nanobubbles (bubble liposomes [BLs]) using ultrasound (US) irradiation in mice. Naked pDNA with BLs was intraperitoneally injected, followed by US irradiation. The injection volume, the duration of US irradiation, and the dose of BLs were optimized. Both BLs and US irradiation were essential to achieve high transgene expression from naked pDNA. We observed transgene expression in the entire peritoneal tissues, including the peritoneal wall, liver, spleen, stomach and small and large intestines. The area of transfection could be controlled with focused US irradiation. There were few changes in the morphology of the peritoneum, the peritoneal function or serum alanine aminotransferase levels, suggesting the safety of BLs with US irradiation. Using a tissue-clearing method, the spatial distribution of transgene expression was evaluated. BLs with US irradiation delivered pDNA to the submesothelial layer in the peritoneal wall, whereas transgene expression was restricted to the surface layer in the liver and stomach. Therefore, BLs with US irradiation could be an effective and safe method of gene transfection to the peritoneum.
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spelling pubmed-82411572021-07-08 Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation Nishimura, Koyo Fumoto, Shintaro Fuchigami, Yuki Hagimori, Masayori Maruyama, Kazuo Kawakami, Shigeru Drug Deliv Research Article In this study, we demonstrate the low toxicity and highly efficient and spatially improved transfection of plasmid DNA (pDNA) with liposomal nanobubbles (bubble liposomes [BLs]) using ultrasound (US) irradiation in mice. Naked pDNA with BLs was intraperitoneally injected, followed by US irradiation. The injection volume, the duration of US irradiation, and the dose of BLs were optimized. Both BLs and US irradiation were essential to achieve high transgene expression from naked pDNA. We observed transgene expression in the entire peritoneal tissues, including the peritoneal wall, liver, spleen, stomach and small and large intestines. The area of transfection could be controlled with focused US irradiation. There were few changes in the morphology of the peritoneum, the peritoneal function or serum alanine aminotransferase levels, suggesting the safety of BLs with US irradiation. Using a tissue-clearing method, the spatial distribution of transgene expression was evaluated. BLs with US irradiation delivered pDNA to the submesothelial layer in the peritoneal wall, whereas transgene expression was restricted to the surface layer in the liver and stomach. Therefore, BLs with US irradiation could be an effective and safe method of gene transfection to the peritoneum. Taylor & Francis 2017-04-27 /pmc/articles/PMC8241157/ /pubmed/28446052 http://dx.doi.org/10.1080/10717544.2017.1319433 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/Licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nishimura, Koyo
Fumoto, Shintaro
Fuchigami, Yuki
Hagimori, Masayori
Maruyama, Kazuo
Kawakami, Shigeru
Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
title Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
title_full Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
title_fullStr Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
title_full_unstemmed Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
title_short Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
title_sort effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241157/
https://www.ncbi.nlm.nih.gov/pubmed/28446052
http://dx.doi.org/10.1080/10717544.2017.1319433
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