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Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris

Aiming at the reutilizing wastewater for algal growth and biomass production, a saline water rejected from reverse osmosis (RO) facility (salinity 67.59 g L(−1)) was used to cultivate the pre-adapted green microalga Chlorella vulgaris. The inoculum was prepared by growing cells in modified BG-11 med...

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Autores principales: Almutairi, Adel W., El-Sayed, Abo El-Khair B., Reda, Marwa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241604/
https://www.ncbi.nlm.nih.gov/pubmed/34220255
http://dx.doi.org/10.1016/j.sjbs.2021.04.007
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author Almutairi, Adel W.
El-Sayed, Abo El-Khair B.
Reda, Marwa M.
author_facet Almutairi, Adel W.
El-Sayed, Abo El-Khair B.
Reda, Marwa M.
author_sort Almutairi, Adel W.
collection PubMed
description Aiming at the reutilizing wastewater for algal growth and biomass production, a saline water rejected from reverse osmosis (RO) facility (salinity 67.59 g L(−1)) was used to cultivate the pre-adapted green microalga Chlorella vulgaris. The inoculum was prepared by growing cells in modified BG-11 medium, and adaptation was performed by applying a gradual increase in salinity (56.0 g L(−1) NaCl and 125 ppm FeSO(4)·7H(2)O) to the culture in 200 L photobioreactor. Experiments using the adapted alga were performed using original-rejected water (ORW) and treated rejected water (TRW) comparing with the recommended growth medium (BG-11). The initial salinity of ORW was chemically reduced to 39.1 g L(−1) to obtain TRW. Vertical photobioreactors (15 L) was used for indoor growth experiments. Growth in BG-11 resulted in 1.23 g L(−1), while the next adaptation growth reached 2.14 g L(−1) of dry biomass. The dry weights of re-cultivated Chlorella after adaptation were 1.49 and 2.19 g L(−1) from ORW and TRW; respectively. The cellular oil content was only 12% when cells grown under control conditions verses to 14.3 and 15.42% with original and treated water, respectively. Induction of stress affected the fatty acid methyl esters (FAMEs) profile and the properties of the resulting biodiesel. The present results indicated that induction of stress by high salinity improves the quality of FAMEs that can be used as a promising biodiesel fuel.
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spelling pubmed-82416042021-07-02 Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris Almutairi, Adel W. El-Sayed, Abo El-Khair B. Reda, Marwa M. Saudi J Biol Sci Original Article Aiming at the reutilizing wastewater for algal growth and biomass production, a saline water rejected from reverse osmosis (RO) facility (salinity 67.59 g L(−1)) was used to cultivate the pre-adapted green microalga Chlorella vulgaris. The inoculum was prepared by growing cells in modified BG-11 medium, and adaptation was performed by applying a gradual increase in salinity (56.0 g L(−1) NaCl and 125 ppm FeSO(4)·7H(2)O) to the culture in 200 L photobioreactor. Experiments using the adapted alga were performed using original-rejected water (ORW) and treated rejected water (TRW) comparing with the recommended growth medium (BG-11). The initial salinity of ORW was chemically reduced to 39.1 g L(−1) to obtain TRW. Vertical photobioreactors (15 L) was used for indoor growth experiments. Growth in BG-11 resulted in 1.23 g L(−1), while the next adaptation growth reached 2.14 g L(−1) of dry biomass. The dry weights of re-cultivated Chlorella after adaptation were 1.49 and 2.19 g L(−1) from ORW and TRW; respectively. The cellular oil content was only 12% when cells grown under control conditions verses to 14.3 and 15.42% with original and treated water, respectively. Induction of stress affected the fatty acid methyl esters (FAMEs) profile and the properties of the resulting biodiesel. The present results indicated that induction of stress by high salinity improves the quality of FAMEs that can be used as a promising biodiesel fuel. Elsevier 2021-07 2021-04-20 /pmc/articles/PMC8241604/ /pubmed/34220255 http://dx.doi.org/10.1016/j.sjbs.2021.04.007 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Almutairi, Adel W.
El-Sayed, Abo El-Khair B.
Reda, Marwa M.
Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris
title Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris
title_full Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris
title_fullStr Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris
title_full_unstemmed Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris
title_short Evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga Chlorella vulgaris
title_sort evaluation of high salinity adaptation for lipid bio-accumulation in the green microalga chlorella vulgaris
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241604/
https://www.ncbi.nlm.nih.gov/pubmed/34220255
http://dx.doi.org/10.1016/j.sjbs.2021.04.007
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