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Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli

Escherichia coli strains are generally sensitive to hydrophobic organic solvents such as n-hexane and cyclohexane. Oxidative stress in E. coli by exposure to these hydrophobic organic solvents has been poorly understood. In the present study, we examined organic solvent tolerance and oxygen radical...

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Autores principales: Doukyu, Noriyuki, Taguchi, Katsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241964/
https://www.ncbi.nlm.nih.gov/pubmed/34189628
http://dx.doi.org/10.1186/s13568-021-01258-w
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author Doukyu, Noriyuki
Taguchi, Katsuya
author_facet Doukyu, Noriyuki
Taguchi, Katsuya
author_sort Doukyu, Noriyuki
collection PubMed
description Escherichia coli strains are generally sensitive to hydrophobic organic solvents such as n-hexane and cyclohexane. Oxidative stress in E. coli by exposure to these hydrophobic organic solvents has been poorly understood. In the present study, we examined organic solvent tolerance and oxygen radical generation in E. coli mutants deficient in reactive oxygen species (ROS)-scavenging enzymes. The organic solvent tolerances in single gene mutants lacking genes encoding superoxide dismutase (sodA, sodB, and sodC), catalase (katE and katG), and alkyl hydroperoxide reductase (ahpCF) were similar to that of parent strain BW25113. We constructed a BW25113-based katE katG double mutant (BW25113∆katE∆katG) and sodA sodB double mutant (BW25113sodA∆sodB). These double-gene mutants were more sensitive to hydrophobic organic solvents than BW25113. In addition, the intracellular ROS levels in E. coli strains increased by the addition of n-hexane or cyclohexane. The ROS levels in BW25113∆katE∆katG and BW25113∆sodA∆sodB induced by exposure to the solvents were higher than that in BW25113. These results suggested that ROS-scavenging enzymes contribute to the maintenance of organic solvent tolerance in E. coli. In addition, the promoter activities of sodA and sodB were significantly increased by exposure to n-hexane. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01258-w.
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spelling pubmed-82419642021-07-13 Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli Doukyu, Noriyuki Taguchi, Katsuya AMB Express Original Article Escherichia coli strains are generally sensitive to hydrophobic organic solvents such as n-hexane and cyclohexane. Oxidative stress in E. coli by exposure to these hydrophobic organic solvents has been poorly understood. In the present study, we examined organic solvent tolerance and oxygen radical generation in E. coli mutants deficient in reactive oxygen species (ROS)-scavenging enzymes. The organic solvent tolerances in single gene mutants lacking genes encoding superoxide dismutase (sodA, sodB, and sodC), catalase (katE and katG), and alkyl hydroperoxide reductase (ahpCF) were similar to that of parent strain BW25113. We constructed a BW25113-based katE katG double mutant (BW25113∆katE∆katG) and sodA sodB double mutant (BW25113sodA∆sodB). These double-gene mutants were more sensitive to hydrophobic organic solvents than BW25113. In addition, the intracellular ROS levels in E. coli strains increased by the addition of n-hexane or cyclohexane. The ROS levels in BW25113∆katE∆katG and BW25113∆sodA∆sodB induced by exposure to the solvents were higher than that in BW25113. These results suggested that ROS-scavenging enzymes contribute to the maintenance of organic solvent tolerance in E. coli. In addition, the promoter activities of sodA and sodB were significantly increased by exposure to n-hexane. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01258-w. Springer Berlin Heidelberg 2021-06-29 /pmc/articles/PMC8241964/ /pubmed/34189628 http://dx.doi.org/10.1186/s13568-021-01258-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Doukyu, Noriyuki
Taguchi, Katsuya
Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli
title Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli
title_full Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli
title_fullStr Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli
title_full_unstemmed Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli
title_short Involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of Escherichia coli
title_sort involvement of catalase and superoxide dismutase in hydrophobic organic solvent tolerance of escherichia coli
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241964/
https://www.ncbi.nlm.nih.gov/pubmed/34189628
http://dx.doi.org/10.1186/s13568-021-01258-w
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