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Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells

Biophysical cues such as osmotic pressure modulate proliferation and growth arrest of bacteria, yeast cells and seeds. In tissues, osmotic regulation takes place through blood and lymphatic capillaries and, at a single cell level, water and osmoregulation play a critical role. However, the effect of...

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Autores principales: Taïeb, Hubert M., Garske, Daniela S., Contzen, Jörg, Gossen, Manfred, Bertinetti, Luca, Robinson, Tom, Cipitria, Amaia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242012/
https://www.ncbi.nlm.nih.gov/pubmed/34188099
http://dx.doi.org/10.1038/s41598-021-92054-w
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author Taïeb, Hubert M.
Garske, Daniela S.
Contzen, Jörg
Gossen, Manfred
Bertinetti, Luca
Robinson, Tom
Cipitria, Amaia
author_facet Taïeb, Hubert M.
Garske, Daniela S.
Contzen, Jörg
Gossen, Manfred
Bertinetti, Luca
Robinson, Tom
Cipitria, Amaia
author_sort Taïeb, Hubert M.
collection PubMed
description Biophysical cues such as osmotic pressure modulate proliferation and growth arrest of bacteria, yeast cells and seeds. In tissues, osmotic regulation takes place through blood and lymphatic capillaries and, at a single cell level, water and osmoregulation play a critical role. However, the effect of osmotic pressure on single cell cycle dynamics remains poorly understood. Here, we investigate the effect of osmotic pressure on single cell cycle dynamics, nuclear growth, proliferation, migration and protein expression, by quantitative time-lapse imaging of single cells genetically modified with fluorescent ubiquitination-based cell cycle indicator 2 (FUCCI2). Single cell data reveals that under hyperosmotic stress, distinct cell subpopulations emerge with impaired nuclear growth, delayed or growth arrested cell cycle and reduced migration. This state is reversible for mild hyperosmotic stress, where cells return to regular cell cycle dynamics, proliferation and migration. Thus, osmotic pressure can modulate the reversible growth arrest and reactivation of human metastatic cells.
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spelling pubmed-82420122021-07-06 Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells Taïeb, Hubert M. Garske, Daniela S. Contzen, Jörg Gossen, Manfred Bertinetti, Luca Robinson, Tom Cipitria, Amaia Sci Rep Article Biophysical cues such as osmotic pressure modulate proliferation and growth arrest of bacteria, yeast cells and seeds. In tissues, osmotic regulation takes place through blood and lymphatic capillaries and, at a single cell level, water and osmoregulation play a critical role. However, the effect of osmotic pressure on single cell cycle dynamics remains poorly understood. Here, we investigate the effect of osmotic pressure on single cell cycle dynamics, nuclear growth, proliferation, migration and protein expression, by quantitative time-lapse imaging of single cells genetically modified with fluorescent ubiquitination-based cell cycle indicator 2 (FUCCI2). Single cell data reveals that under hyperosmotic stress, distinct cell subpopulations emerge with impaired nuclear growth, delayed or growth arrested cell cycle and reduced migration. This state is reversible for mild hyperosmotic stress, where cells return to regular cell cycle dynamics, proliferation and migration. Thus, osmotic pressure can modulate the reversible growth arrest and reactivation of human metastatic cells. Nature Publishing Group UK 2021-06-29 /pmc/articles/PMC8242012/ /pubmed/34188099 http://dx.doi.org/10.1038/s41598-021-92054-w Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Taïeb, Hubert M.
Garske, Daniela S.
Contzen, Jörg
Gossen, Manfred
Bertinetti, Luca
Robinson, Tom
Cipitria, Amaia
Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_full Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_fullStr Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_full_unstemmed Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_short Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_sort osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242012/
https://www.ncbi.nlm.nih.gov/pubmed/34188099
http://dx.doi.org/10.1038/s41598-021-92054-w
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