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High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan

OBJECTIVES: To assess the prevalence and antibiotic resistance profile of Mycoplasma genitalium detected from urogenital/rectal swab samples obtained from MSM in Tokyo, Japan. METHODS: We performed PCR-based screening for M. genitalium urogenital/rectal infection in 982 asymptomatic MSM between 1 Ja...

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Autores principales: Ando, Naokatsu, Mizushima, Daisuke, Takano, Misao, Mitobe, Morika, Miyake, Hirofumi, Yokoyama, Keiko, Sadamasu, Kenji, Aoki, Takahiro, Watanabe, Koji, Uemura, Haruka, Yanagawa, Yasuaki, Gatanaga, Hiroyuki, Oka, Shinichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242132/
https://www.ncbi.nlm.nih.gov/pubmed/34223146
http://dx.doi.org/10.1093/jacamr/dlab091
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author Ando, Naokatsu
Mizushima, Daisuke
Takano, Misao
Mitobe, Morika
Miyake, Hirofumi
Yokoyama, Keiko
Sadamasu, Kenji
Aoki, Takahiro
Watanabe, Koji
Uemura, Haruka
Yanagawa, Yasuaki
Gatanaga, Hiroyuki
Oka, Shinichi
author_facet Ando, Naokatsu
Mizushima, Daisuke
Takano, Misao
Mitobe, Morika
Miyake, Hirofumi
Yokoyama, Keiko
Sadamasu, Kenji
Aoki, Takahiro
Watanabe, Koji
Uemura, Haruka
Yanagawa, Yasuaki
Gatanaga, Hiroyuki
Oka, Shinichi
author_sort Ando, Naokatsu
collection PubMed
description OBJECTIVES: To assess the prevalence and antibiotic resistance profile of Mycoplasma genitalium detected from urogenital/rectal swab samples obtained from MSM in Tokyo, Japan. METHODS: We performed PCR-based screening for M. genitalium urogenital/rectal infection in 982 asymptomatic MSM between 1 January 2019 and 5 November 2020. Mutations in the antibiotic resistance-associated genes gyrA and parC and the 23S rRNA of M. genitalium were analysed. RESULTS: The prevalence of M. genitalium infection was 6.1%: the prevalence of rectal and urogenital infection was 4.7% and 1.4%, respectively. Among the cases, 48 were successfully analysed for 23S rRNA, 41 for parC mutations and 37 for gyrA mutations. Macrolide- and quinolone-resistance associated mutations (23S rRNA and parC mutations) were observed in 43 (89.6%) and 28 (68.3%) cases, respectively. The quinolone-resistance associated mutation-harbouring variants also harboured macrolide-resistance associated mutations. The S83I mutation in the parC gene was most commonly identified (24 cases, 58.5%), and its combination with M95I or D99N mutation in the gyrA gene was observed in 9 of 36 successfully analysed cases (25.0%). No significant association was observed between the presence of antibiotic resistance and antibiotic exposure for either macrolides or fluoroquinolones (P = 0.785 and 0.402, respectively). CONCLUSIONS: In Tokyo, there is an alarmingly high prevalence of M. genitalium harbouring macrolide and/or quinolone resistance-associated mutations in MSM, irrespective of antibiotic exposure. The high prevalence of M. genitalium strains with both parC and gyrA mutations limits the efficacy of sitafloxacin. Therefore, suitable alternatives are required to treat such M. genitalium infections.
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spelling pubmed-82421322021-07-02 High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan Ando, Naokatsu Mizushima, Daisuke Takano, Misao Mitobe, Morika Miyake, Hirofumi Yokoyama, Keiko Sadamasu, Kenji Aoki, Takahiro Watanabe, Koji Uemura, Haruka Yanagawa, Yasuaki Gatanaga, Hiroyuki Oka, Shinichi JAC Antimicrob Resist Brief Report OBJECTIVES: To assess the prevalence and antibiotic resistance profile of Mycoplasma genitalium detected from urogenital/rectal swab samples obtained from MSM in Tokyo, Japan. METHODS: We performed PCR-based screening for M. genitalium urogenital/rectal infection in 982 asymptomatic MSM between 1 January 2019 and 5 November 2020. Mutations in the antibiotic resistance-associated genes gyrA and parC and the 23S rRNA of M. genitalium were analysed. RESULTS: The prevalence of M. genitalium infection was 6.1%: the prevalence of rectal and urogenital infection was 4.7% and 1.4%, respectively. Among the cases, 48 were successfully analysed for 23S rRNA, 41 for parC mutations and 37 for gyrA mutations. Macrolide- and quinolone-resistance associated mutations (23S rRNA and parC mutations) were observed in 43 (89.6%) and 28 (68.3%) cases, respectively. The quinolone-resistance associated mutation-harbouring variants also harboured macrolide-resistance associated mutations. The S83I mutation in the parC gene was most commonly identified (24 cases, 58.5%), and its combination with M95I or D99N mutation in the gyrA gene was observed in 9 of 36 successfully analysed cases (25.0%). No significant association was observed between the presence of antibiotic resistance and antibiotic exposure for either macrolides or fluoroquinolones (P = 0.785 and 0.402, respectively). CONCLUSIONS: In Tokyo, there is an alarmingly high prevalence of M. genitalium harbouring macrolide and/or quinolone resistance-associated mutations in MSM, irrespective of antibiotic exposure. The high prevalence of M. genitalium strains with both parC and gyrA mutations limits the efficacy of sitafloxacin. Therefore, suitable alternatives are required to treat such M. genitalium infections. Oxford University Press 2021-06-30 /pmc/articles/PMC8242132/ /pubmed/34223146 http://dx.doi.org/10.1093/jacamr/dlab091 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Brief Report
Ando, Naokatsu
Mizushima, Daisuke
Takano, Misao
Mitobe, Morika
Miyake, Hirofumi
Yokoyama, Keiko
Sadamasu, Kenji
Aoki, Takahiro
Watanabe, Koji
Uemura, Haruka
Yanagawa, Yasuaki
Gatanaga, Hiroyuki
Oka, Shinichi
High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan
title High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan
title_full High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan
title_fullStr High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan
title_full_unstemmed High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan
title_short High prevalence of circulating dual-class resistant Mycoplasma genitalium in asymptomatic MSM in Tokyo, Japan
title_sort high prevalence of circulating dual-class resistant mycoplasma genitalium in asymptomatic msm in tokyo, japan
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242132/
https://www.ncbi.nlm.nih.gov/pubmed/34223146
http://dx.doi.org/10.1093/jacamr/dlab091
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