Cargando…
A Non-Coding RNA Network Involved in KSHV Tumorigenesis
Regulatory pathways involving non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA), have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242244/ https://www.ncbi.nlm.nih.gov/pubmed/34222014 http://dx.doi.org/10.3389/fonc.2021.687629 |
_version_ | 1783715592036941824 |
---|---|
author | Naipauer, Julián García Solá, Martín E. Salyakina, Daria Rosario, Santas Williams, Sion Coso, Omar Abba, Martín C. Mesri, Enrique A. Lacunza, Ezequiel |
author_facet | Naipauer, Julián García Solá, Martín E. Salyakina, Daria Rosario, Santas Williams, Sion Coso, Omar Abba, Martín C. Mesri, Enrique A. Lacunza, Ezequiel |
author_sort | Naipauer, Julián |
collection | PubMed |
description | Regulatory pathways involving non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA), have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors, we have analyzed the host and viral transcriptome to uncover the role lncRNA-miRNA-mRNA driven networks in KSHV tumorigenesis. The integration of the differentially expressed ncRNAs, with an exhaustive computational analysis of their experimentally supported targets, led us to dissect complex networks integrated by the cancer-related lncRNAs Malat1, Neat1, H19, Meg3, and their associated miRNA-target pairs. These networks would modulate pathways related to KSHV pathogenesis, such as viral carcinogenesis, p53 signaling, RNA surveillance, and cell cycle control. Finally, the ncRNA-mRNA analysis allowed us to develop signatures that can be used to an appropriate identification of druggable gene or networks defining relevant AIDS-KS therapeutic targets. |
format | Online Article Text |
id | pubmed-8242244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82422442021-07-01 A Non-Coding RNA Network Involved in KSHV Tumorigenesis Naipauer, Julián García Solá, Martín E. Salyakina, Daria Rosario, Santas Williams, Sion Coso, Omar Abba, Martín C. Mesri, Enrique A. Lacunza, Ezequiel Front Oncol Oncology Regulatory pathways involving non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA), have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors, we have analyzed the host and viral transcriptome to uncover the role lncRNA-miRNA-mRNA driven networks in KSHV tumorigenesis. The integration of the differentially expressed ncRNAs, with an exhaustive computational analysis of their experimentally supported targets, led us to dissect complex networks integrated by the cancer-related lncRNAs Malat1, Neat1, H19, Meg3, and their associated miRNA-target pairs. These networks would modulate pathways related to KSHV pathogenesis, such as viral carcinogenesis, p53 signaling, RNA surveillance, and cell cycle control. Finally, the ncRNA-mRNA analysis allowed us to develop signatures that can be used to an appropriate identification of druggable gene or networks defining relevant AIDS-KS therapeutic targets. Frontiers Media S.A. 2021-06-16 /pmc/articles/PMC8242244/ /pubmed/34222014 http://dx.doi.org/10.3389/fonc.2021.687629 Text en Copyright © 2021 Naipauer, García Solá, Salyakina, Rosario, Williams, Coso, Abba, Mesri and Lacunza https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Naipauer, Julián García Solá, Martín E. Salyakina, Daria Rosario, Santas Williams, Sion Coso, Omar Abba, Martín C. Mesri, Enrique A. Lacunza, Ezequiel A Non-Coding RNA Network Involved in KSHV Tumorigenesis |
title | A Non-Coding RNA Network Involved in KSHV Tumorigenesis |
title_full | A Non-Coding RNA Network Involved in KSHV Tumorigenesis |
title_fullStr | A Non-Coding RNA Network Involved in KSHV Tumorigenesis |
title_full_unstemmed | A Non-Coding RNA Network Involved in KSHV Tumorigenesis |
title_short | A Non-Coding RNA Network Involved in KSHV Tumorigenesis |
title_sort | non-coding rna network involved in kshv tumorigenesis |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242244/ https://www.ncbi.nlm.nih.gov/pubmed/34222014 http://dx.doi.org/10.3389/fonc.2021.687629 |
work_keys_str_mv | AT naipauerjulian anoncodingrnanetworkinvolvedinkshvtumorigenesis AT garciasolamartine anoncodingrnanetworkinvolvedinkshvtumorigenesis AT salyakinadaria anoncodingrnanetworkinvolvedinkshvtumorigenesis AT rosariosantas anoncodingrnanetworkinvolvedinkshvtumorigenesis AT williamssion anoncodingrnanetworkinvolvedinkshvtumorigenesis AT cosoomar anoncodingrnanetworkinvolvedinkshvtumorigenesis AT abbamartinc anoncodingrnanetworkinvolvedinkshvtumorigenesis AT mesrienriquea anoncodingrnanetworkinvolvedinkshvtumorigenesis AT lacunzaezequiel anoncodingrnanetworkinvolvedinkshvtumorigenesis AT naipauerjulian noncodingrnanetworkinvolvedinkshvtumorigenesis AT garciasolamartine noncodingrnanetworkinvolvedinkshvtumorigenesis AT salyakinadaria noncodingrnanetworkinvolvedinkshvtumorigenesis AT rosariosantas noncodingrnanetworkinvolvedinkshvtumorigenesis AT williamssion noncodingrnanetworkinvolvedinkshvtumorigenesis AT cosoomar noncodingrnanetworkinvolvedinkshvtumorigenesis AT abbamartinc noncodingrnanetworkinvolvedinkshvtumorigenesis AT mesrienriquea noncodingrnanetworkinvolvedinkshvtumorigenesis AT lacunzaezequiel noncodingrnanetworkinvolvedinkshvtumorigenesis |