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A Non-Coding RNA Network Involved in KSHV Tumorigenesis

Regulatory pathways involving non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA), have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors...

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Autores principales: Naipauer, Julián, García Solá, Martín E., Salyakina, Daria, Rosario, Santas, Williams, Sion, Coso, Omar, Abba, Martín C., Mesri, Enrique A., Lacunza, Ezequiel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242244/
https://www.ncbi.nlm.nih.gov/pubmed/34222014
http://dx.doi.org/10.3389/fonc.2021.687629
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author Naipauer, Julián
García Solá, Martín E.
Salyakina, Daria
Rosario, Santas
Williams, Sion
Coso, Omar
Abba, Martín C.
Mesri, Enrique A.
Lacunza, Ezequiel
author_facet Naipauer, Julián
García Solá, Martín E.
Salyakina, Daria
Rosario, Santas
Williams, Sion
Coso, Omar
Abba, Martín C.
Mesri, Enrique A.
Lacunza, Ezequiel
author_sort Naipauer, Julián
collection PubMed
description Regulatory pathways involving non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA), have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors, we have analyzed the host and viral transcriptome to uncover the role lncRNA-miRNA-mRNA driven networks in KSHV tumorigenesis. The integration of the differentially expressed ncRNAs, with an exhaustive computational analysis of their experimentally supported targets, led us to dissect complex networks integrated by the cancer-related lncRNAs Malat1, Neat1, H19, Meg3, and their associated miRNA-target pairs. These networks would modulate pathways related to KSHV pathogenesis, such as viral carcinogenesis, p53 signaling, RNA surveillance, and cell cycle control. Finally, the ncRNA-mRNA analysis allowed us to develop signatures that can be used to an appropriate identification of druggable gene or networks defining relevant AIDS-KS therapeutic targets.
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spelling pubmed-82422442021-07-01 A Non-Coding RNA Network Involved in KSHV Tumorigenesis Naipauer, Julián García Solá, Martín E. Salyakina, Daria Rosario, Santas Williams, Sion Coso, Omar Abba, Martín C. Mesri, Enrique A. Lacunza, Ezequiel Front Oncol Oncology Regulatory pathways involving non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA), have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors, we have analyzed the host and viral transcriptome to uncover the role lncRNA-miRNA-mRNA driven networks in KSHV tumorigenesis. The integration of the differentially expressed ncRNAs, with an exhaustive computational analysis of their experimentally supported targets, led us to dissect complex networks integrated by the cancer-related lncRNAs Malat1, Neat1, H19, Meg3, and their associated miRNA-target pairs. These networks would modulate pathways related to KSHV pathogenesis, such as viral carcinogenesis, p53 signaling, RNA surveillance, and cell cycle control. Finally, the ncRNA-mRNA analysis allowed us to develop signatures that can be used to an appropriate identification of druggable gene or networks defining relevant AIDS-KS therapeutic targets. Frontiers Media S.A. 2021-06-16 /pmc/articles/PMC8242244/ /pubmed/34222014 http://dx.doi.org/10.3389/fonc.2021.687629 Text en Copyright © 2021 Naipauer, García Solá, Salyakina, Rosario, Williams, Coso, Abba, Mesri and Lacunza https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Naipauer, Julián
García Solá, Martín E.
Salyakina, Daria
Rosario, Santas
Williams, Sion
Coso, Omar
Abba, Martín C.
Mesri, Enrique A.
Lacunza, Ezequiel
A Non-Coding RNA Network Involved in KSHV Tumorigenesis
title A Non-Coding RNA Network Involved in KSHV Tumorigenesis
title_full A Non-Coding RNA Network Involved in KSHV Tumorigenesis
title_fullStr A Non-Coding RNA Network Involved in KSHV Tumorigenesis
title_full_unstemmed A Non-Coding RNA Network Involved in KSHV Tumorigenesis
title_short A Non-Coding RNA Network Involved in KSHV Tumorigenesis
title_sort non-coding rna network involved in kshv tumorigenesis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242244/
https://www.ncbi.nlm.nih.gov/pubmed/34222014
http://dx.doi.org/10.3389/fonc.2021.687629
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