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A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1

MicroRNA (miRNA) is an important regulator for gene expression. Recent studies showed that some heterogenous miRNAs derived from both parasite and plant can regulate expression of mammalian gene in a cross-species or even a cross-kingdom manner. Here, we identified a Schistosoma japonicum miRNA (des...

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Autores principales: Hu, Chao, Li, Yuzhen, Pan, Danting, Wang, Jing, Zhu, Liufang, Lin, Yu, Zhu, Shanli, Pan, Weiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242254/
https://www.ncbi.nlm.nih.gov/pubmed/34221971
http://dx.doi.org/10.3389/fonc.2021.652395
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author Hu, Chao
Li, Yuzhen
Pan, Danting
Wang, Jing
Zhu, Liufang
Lin, Yu
Zhu, Shanli
Pan, Weiqing
author_facet Hu, Chao
Li, Yuzhen
Pan, Danting
Wang, Jing
Zhu, Liufang
Lin, Yu
Zhu, Shanli
Pan, Weiqing
author_sort Hu, Chao
collection PubMed
description MicroRNA (miRNA) is an important regulator for gene expression. Recent studies showed that some heterogenous miRNAs derived from both parasite and plant can regulate expression of mammalian gene in a cross-species or even a cross-kingdom manner. Here, we identified a Schistosoma japonicum miRNA (designated as sja-miR-61) that is present in the hepatocyte of mice infected with the parasite. The sja-miR-61 mimics significantly inhibited the migration of both mouse and human hepatoma cells in vitro. In a xenograft animal model, significant reductions of the tumor volume and weight were observed in mice inoculated with hepatoma cells transfected with sja-miR-61 mimics compared to the controls. We found that the in vivo inhibition of tumor growth was through its anti-angiogenesis activity. Mechanically, we identified the phosphoglycerate mutase 1 (PGAM1) gene as a target of sja-miR-61 and found that the sja-miR-61-mediated suppression of cell migration and anti-angiogenesis by cross-species down-regulation of PGAM1 expression. These data indicated that sja-miR-61 is a tumor suppressor miRNA that may have therapeutic potential for human cancers.
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spelling pubmed-82422542021-07-01 A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1 Hu, Chao Li, Yuzhen Pan, Danting Wang, Jing Zhu, Liufang Lin, Yu Zhu, Shanli Pan, Weiqing Front Oncol Oncology MicroRNA (miRNA) is an important regulator for gene expression. Recent studies showed that some heterogenous miRNAs derived from both parasite and plant can regulate expression of mammalian gene in a cross-species or even a cross-kingdom manner. Here, we identified a Schistosoma japonicum miRNA (designated as sja-miR-61) that is present in the hepatocyte of mice infected with the parasite. The sja-miR-61 mimics significantly inhibited the migration of both mouse and human hepatoma cells in vitro. In a xenograft animal model, significant reductions of the tumor volume and weight were observed in mice inoculated with hepatoma cells transfected with sja-miR-61 mimics compared to the controls. We found that the in vivo inhibition of tumor growth was through its anti-angiogenesis activity. Mechanically, we identified the phosphoglycerate mutase 1 (PGAM1) gene as a target of sja-miR-61 and found that the sja-miR-61-mediated suppression of cell migration and anti-angiogenesis by cross-species down-regulation of PGAM1 expression. These data indicated that sja-miR-61 is a tumor suppressor miRNA that may have therapeutic potential for human cancers. Frontiers Media S.A. 2021-06-16 /pmc/articles/PMC8242254/ /pubmed/34221971 http://dx.doi.org/10.3389/fonc.2021.652395 Text en Copyright © 2021 Hu, Li, Pan, Wang, Zhu, Lin, Zhu and Pan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Hu, Chao
Li, Yuzhen
Pan, Danting
Wang, Jing
Zhu, Liufang
Lin, Yu
Zhu, Shanli
Pan, Weiqing
A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1
title A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1
title_full A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1
title_fullStr A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1
title_full_unstemmed A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1
title_short A Schistosoma japonicum MicroRNA Exerts Antitumor Effects Through Inhibition of Both Cell Migration and Angiogenesis by Targeting PGAM1
title_sort schistosoma japonicum microrna exerts antitumor effects through inhibition of both cell migration and angiogenesis by targeting pgam1
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242254/
https://www.ncbi.nlm.nih.gov/pubmed/34221971
http://dx.doi.org/10.3389/fonc.2021.652395
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