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Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods
Serological testing for anti‐severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) antibodies is used to detect ongoing or past SARS‐CoV‐2 infections. To study the kinetics of anti‐SARS‐CoV‐2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242665/ https://www.ncbi.nlm.nih.gov/pubmed/34061367 http://dx.doi.org/10.1002/jmv.27113 |
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author | Emmerich, Petra von Possel, Ronald Hemmer, Christoph Josef Fritzsche, Carlos Geerdes‐Fenge, Hilte Menge, Babett Messing, Claudia Borchardt‐Lohölter, Viola Deschermeier, Christina Steinhagen, Katja |
author_facet | Emmerich, Petra von Possel, Ronald Hemmer, Christoph Josef Fritzsche, Carlos Geerdes‐Fenge, Hilte Menge, Babett Messing, Claudia Borchardt‐Lohölter, Viola Deschermeier, Christina Steinhagen, Katja |
author_sort | Emmerich, Petra |
collection | PubMed |
description | Serological testing for anti‐severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) antibodies is used to detect ongoing or past SARS‐CoV‐2 infections. To study the kinetics of anti‐SARS‐CoV‐2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with polymerase chain reaction‐confirmed coronavirus disease 2019 (COVID‐19) and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) antibodies using indirect immunofluorescence testing (IIFT) based on SARS‐CoV‐2‐infected cells. They were further tested for antibodies against the S1 domain of the SARS‐CoV‐2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using enzyme‐linked immunosorbent assays. The assay specificities were 94.4%–100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were the highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed the highest sensitivities due to the use of the whole SARS‐CoV‐2 as substrate and provided information on whether or not the individual has been infected with SARS‐CoV‐2. Enzyme‐linked immunosorbent assays provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS‐CoV‐2. |
format | Online Article Text |
id | pubmed-8242665 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82426652021-07-01 Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods Emmerich, Petra von Possel, Ronald Hemmer, Christoph Josef Fritzsche, Carlos Geerdes‐Fenge, Hilte Menge, Babett Messing, Claudia Borchardt‐Lohölter, Viola Deschermeier, Christina Steinhagen, Katja J Med Virol Research Articles Serological testing for anti‐severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) antibodies is used to detect ongoing or past SARS‐CoV‐2 infections. To study the kinetics of anti‐SARS‐CoV‐2 antibodies and to assess the diagnostic performances of eight serological assays, we used 129 serum samples collected on known days post symptom onset (dpso) from 42 patients with polymerase chain reaction‐confirmed coronavirus disease 2019 (COVID‐19) and 54 serum samples from healthy blood donors, and children infected with seasonal coronaviruses. The sera were analyzed for the presence of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) antibodies using indirect immunofluorescence testing (IIFT) based on SARS‐CoV‐2‐infected cells. They were further tested for antibodies against the S1 domain of the SARS‐CoV‐2 spike protein (IgG, IgA) and against the viral nucleocapsid protein (IgG, IgM) using enzyme‐linked immunosorbent assays. The assay specificities were 94.4%–100%. The sensitivities varied largely between assays, reflecting their respective purposes. The sensitivities of IgA and IgM assays were the highest between 11 and 20 dpso, whereas the sensitivities of IgG assays peaked between 20 and 60 dpso. IIFT showed the highest sensitivities due to the use of the whole SARS‐CoV‐2 as substrate and provided information on whether or not the individual has been infected with SARS‐CoV‐2. Enzyme‐linked immunosorbent assays provided further information about both the prevalence and concentration of specific antibodies against selected antigens of SARS‐CoV‐2. John Wiley and Sons Inc. 2021-06-11 2021-10 /pmc/articles/PMC8242665/ /pubmed/34061367 http://dx.doi.org/10.1002/jmv.27113 Text en © 2021 The Authors. Journal of Medical Virology Published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Emmerich, Petra von Possel, Ronald Hemmer, Christoph Josef Fritzsche, Carlos Geerdes‐Fenge, Hilte Menge, Babett Messing, Claudia Borchardt‐Lohölter, Viola Deschermeier, Christina Steinhagen, Katja Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods |
title | Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods |
title_full | Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods |
title_fullStr | Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods |
title_full_unstemmed | Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods |
title_short | Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods |
title_sort | longitudinal detection of sars‐cov‐2‐specific antibody responses with different serological methods |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242665/ https://www.ncbi.nlm.nih.gov/pubmed/34061367 http://dx.doi.org/10.1002/jmv.27113 |
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