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In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-...

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Detalles Bibliográficos
Autores principales: Shen, Shensi, Girault, Isabelle, Malka-Mahieu, Hélène, Robert, Caroline, Vagner, Stéphan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243118/
https://www.ncbi.nlm.nih.gov/pubmed/34223196
http://dx.doi.org/10.1016/j.xpro.2021.100621