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In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues
The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-...
Autores principales: | Shen, Shensi, Girault, Isabelle, Malka-Mahieu, Hélène, Robert, Caroline, Vagner, Stéphan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243118/ https://www.ncbi.nlm.nih.gov/pubmed/34223196 http://dx.doi.org/10.1016/j.xpro.2021.100621 |
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