Cargando…

Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor

BACKGROUND: The network of intermediate filament proteins underlying the inner nuclear membrane forms the nuclear lamin. A- and B-type lamins are the major components of the nuclear lamina. Lamins function in many nuclear activities. The role of lamin A and transcription factors (NF-kB) as anti-apop...

Descripción completa

Detalles Bibliográficos
Autores principales: Gumina, Stefano, Peruzzi, Barbara, Leopizzi, Martina, Porta, Natale, Di Maio, Valeria, Rocca, Carlo Della, Candela, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243552/
https://www.ncbi.nlm.nih.gov/pubmed/34193225
http://dx.doi.org/10.1186/s13018-021-02569-1
_version_ 1783715772637380608
author Gumina, Stefano
Peruzzi, Barbara
Leopizzi, Martina
Porta, Natale
Di Maio, Valeria
Rocca, Carlo Della
Candela, Vittorio
author_facet Gumina, Stefano
Peruzzi, Barbara
Leopizzi, Martina
Porta, Natale
Di Maio, Valeria
Rocca, Carlo Della
Candela, Vittorio
author_sort Gumina, Stefano
collection PubMed
description BACKGROUND: The network of intermediate filament proteins underlying the inner nuclear membrane forms the nuclear lamin. A- and B-type lamins are the major components of the nuclear lamina. Lamins function in many nuclear activities. The role of lamin A and transcription factors (NF-kB) as anti-apoptotic is well documented. Recently, lamin A has also been considered as a mechanosensor protein that is able to maintain nuclear integrity from mechanical insults. We aimed to verify how lamin A expression varies in healthy cuff cells and in those with different-sized tears where various mechanical stresses are present. METHODS: Forty-three patients with rotator cuff tear (RCT) [23M–20F, mean age (SD): 63.5 (6.1)] were enrolled. Tissue samples excised from the most medial point of tear margins were analyzed for lamin A expression by immunohistochemistry. Controls were represented by samples obtained by normal supraspinatus tendons excised from patients submitted to reverse shoulder prosthesis implant [8M–7F, mean age (SD): 67.9 (7.1)]. The intensity of staining was graded, and an H-score was assigned. Statistical analysis was performed. RESULTS: Our study revealed a moderate intensity of lamin A in the healthy cuff tendons, a higher expression of this protein in the small tears, and a significant decrease of lamin A with increasing tear size (p < 0.0001). CONCLUSIONS: Our study emphasizes the importance of early repair of small RCTs since nuclear stability is maintained, and the cellular function is protected by lamin A overexpression. High re-tear of massive cuff repair could be due to cellular apoptosis and nuclear modifications induced by lamin A lack. LEVEL OF EVIDENCE: III
format Online
Article
Text
id pubmed-8243552
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-82435522021-06-30 Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor Gumina, Stefano Peruzzi, Barbara Leopizzi, Martina Porta, Natale Di Maio, Valeria Rocca, Carlo Della Candela, Vittorio J Orthop Surg Res Research Article BACKGROUND: The network of intermediate filament proteins underlying the inner nuclear membrane forms the nuclear lamin. A- and B-type lamins are the major components of the nuclear lamina. Lamins function in many nuclear activities. The role of lamin A and transcription factors (NF-kB) as anti-apoptotic is well documented. Recently, lamin A has also been considered as a mechanosensor protein that is able to maintain nuclear integrity from mechanical insults. We aimed to verify how lamin A expression varies in healthy cuff cells and in those with different-sized tears where various mechanical stresses are present. METHODS: Forty-three patients with rotator cuff tear (RCT) [23M–20F, mean age (SD): 63.5 (6.1)] were enrolled. Tissue samples excised from the most medial point of tear margins were analyzed for lamin A expression by immunohistochemistry. Controls were represented by samples obtained by normal supraspinatus tendons excised from patients submitted to reverse shoulder prosthesis implant [8M–7F, mean age (SD): 67.9 (7.1)]. The intensity of staining was graded, and an H-score was assigned. Statistical analysis was performed. RESULTS: Our study revealed a moderate intensity of lamin A in the healthy cuff tendons, a higher expression of this protein in the small tears, and a significant decrease of lamin A with increasing tear size (p < 0.0001). CONCLUSIONS: Our study emphasizes the importance of early repair of small RCTs since nuclear stability is maintained, and the cellular function is protected by lamin A overexpression. High re-tear of massive cuff repair could be due to cellular apoptosis and nuclear modifications induced by lamin A lack. LEVEL OF EVIDENCE: III BioMed Central 2021-06-30 /pmc/articles/PMC8243552/ /pubmed/34193225 http://dx.doi.org/10.1186/s13018-021-02569-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Gumina, Stefano
Peruzzi, Barbara
Leopizzi, Martina
Porta, Natale
Di Maio, Valeria
Rocca, Carlo Della
Candela, Vittorio
Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor
title Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor
title_full Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor
title_fullStr Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor
title_full_unstemmed Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor
title_short Nuclear lamin A in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor
title_sort nuclear lamin a in rotator cuff tear margin tenocytes: an antiapoptotic and cell mechanostat factor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243552/
https://www.ncbi.nlm.nih.gov/pubmed/34193225
http://dx.doi.org/10.1186/s13018-021-02569-1
work_keys_str_mv AT guminastefano nuclearlaminainrotatorcufftearmargintenocytesanantiapoptoticandcellmechanostatfactor
AT peruzzibarbara nuclearlaminainrotatorcufftearmargintenocytesanantiapoptoticandcellmechanostatfactor
AT leopizzimartina nuclearlaminainrotatorcufftearmargintenocytesanantiapoptoticandcellmechanostatfactor
AT portanatale nuclearlaminainrotatorcufftearmargintenocytesanantiapoptoticandcellmechanostatfactor
AT dimaiovaleria nuclearlaminainrotatorcufftearmargintenocytesanantiapoptoticandcellmechanostatfactor
AT roccacarlodella nuclearlaminainrotatorcufftearmargintenocytesanantiapoptoticandcellmechanostatfactor
AT candelavittorio nuclearlaminainrotatorcufftearmargintenocytesanantiapoptoticandcellmechanostatfactor