3D super-resolution deep-tissue imaging in living mice

Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light sc...

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Autores principales: Velasco, Mary Grace M., Zhang, Mengyang, Antonello, Jacopo, Yuan, Peng, Allgeyer, Edward S., May, Dennis, M’Saad, Ons, Kidd, Phylicia, Barentine, Andrew E. S., Greco, Valentina, Grutzendler, Jaime, Booth, Martin J., Bewersdorf, Joerg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243577/
https://www.ncbi.nlm.nih.gov/pubmed/34239948
http://dx.doi.org/10.1364/OPTICA.416841
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author Velasco, Mary Grace M.
Zhang, Mengyang
Antonello, Jacopo
Yuan, Peng
Allgeyer, Edward S.
May, Dennis
M’Saad, Ons
Kidd, Phylicia
Barentine, Andrew E. S.
Greco, Valentina
Grutzendler, Jaime
Booth, Martin J.
Bewersdorf, Joerg
author_facet Velasco, Mary Grace M.
Zhang, Mengyang
Antonello, Jacopo
Yuan, Peng
Allgeyer, Edward S.
May, Dennis
M’Saad, Ons
Kidd, Phylicia
Barentine, Andrew E. S.
Greco, Valentina
Grutzendler, Jaime
Booth, Martin J.
Bewersdorf, Joerg
author_sort Velasco, Mary Grace M.
collection PubMed
description Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.
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spelling pubmed-82435772021-07-06 3D super-resolution deep-tissue imaging in living mice Velasco, Mary Grace M. Zhang, Mengyang Antonello, Jacopo Yuan, Peng Allgeyer, Edward S. May, Dennis M’Saad, Ons Kidd, Phylicia Barentine, Andrew E. S. Greco, Valentina Grutzendler, Jaime Booth, Martin J. Bewersdorf, Joerg Optica Article Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse. Optical Society of America 2021-03-25 /pmc/articles/PMC8243577/ /pubmed/34239948 http://dx.doi.org/10.1364/OPTICA.416841 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/4.0/ 2334-2536/21/040442-09
spellingShingle Article
Velasco, Mary Grace M.
Zhang, Mengyang
Antonello, Jacopo
Yuan, Peng
Allgeyer, Edward S.
May, Dennis
M’Saad, Ons
Kidd, Phylicia
Barentine, Andrew E. S.
Greco, Valentina
Grutzendler, Jaime
Booth, Martin J.
Bewersdorf, Joerg
3D super-resolution deep-tissue imaging in living mice
title 3D super-resolution deep-tissue imaging in living mice
title_full 3D super-resolution deep-tissue imaging in living mice
title_fullStr 3D super-resolution deep-tissue imaging in living mice
title_full_unstemmed 3D super-resolution deep-tissue imaging in living mice
title_short 3D super-resolution deep-tissue imaging in living mice
title_sort 3d super-resolution deep-tissue imaging in living mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243577/
https://www.ncbi.nlm.nih.gov/pubmed/34239948
http://dx.doi.org/10.1364/OPTICA.416841
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