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Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia

BACKGROUND AND AIM: Subclinical mastitis is an udder infection devoid of clinical symptoms, and Staphylococcus aureus is one of the bacteria causing this disease. This study aimed to identify and determine the prevalence and antibiotic susceptibility of methicillin-resistant S. aureus (MRSA)-associa...

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Autores principales: Qolbaini, Evi Nur, Khoeri, Miftahudin Majid, Salsabila, Korrie, Paramaiswari, Wisiva Tofriska, Tafroji, Wisnu, Artika, I. Made, Safari, Dodi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243663/
https://www.ncbi.nlm.nih.gov/pubmed/34220119
http://dx.doi.org/10.14202/vetworld.2021.1180-1184
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author Qolbaini, Evi Nur
Khoeri, Miftahudin Majid
Salsabila, Korrie
Paramaiswari, Wisiva Tofriska
Tafroji, Wisnu
Artika, I. Made
Safari, Dodi
author_facet Qolbaini, Evi Nur
Khoeri, Miftahudin Majid
Salsabila, Korrie
Paramaiswari, Wisiva Tofriska
Tafroji, Wisnu
Artika, I. Made
Safari, Dodi
author_sort Qolbaini, Evi Nur
collection PubMed
description BACKGROUND AND AIM: Subclinical mastitis is an udder infection devoid of clinical symptoms, and Staphylococcus aureus is one of the bacteria causing this disease. This study aimed to identify and determine the prevalence and antibiotic susceptibility of methicillin-resistant S. aureus (MRSA)-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia. MATERIALS AND METHODS: S. aureus was isolated from subclinical mastitis milk specimens. All strains were confirmed by polymerase chain reaction-based detection of staphylococcal nuc, and MRSA was confirmed by the presence of mecA. Antibiotic susceptibility was determined using the disk diffusion method. RESULTS: From 86 milk samples, 49 isolates (57%) were confirmed as S. aureus. All S. aureus isolates were susceptible to tetracycline, gentamicin, chloramphenicol, erythromycin, and trimethoprim/sulfamethoxazole. Nine isolates were identified as MRSA (10.5%). CONCLUSION: In this study, we reported MRSA-associated subclinical mastitis in Bogor, Indonesia.
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spelling pubmed-82436632021-07-02 Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia Qolbaini, Evi Nur Khoeri, Miftahudin Majid Salsabila, Korrie Paramaiswari, Wisiva Tofriska Tafroji, Wisnu Artika, I. Made Safari, Dodi Vet World Research Article BACKGROUND AND AIM: Subclinical mastitis is an udder infection devoid of clinical symptoms, and Staphylococcus aureus is one of the bacteria causing this disease. This study aimed to identify and determine the prevalence and antibiotic susceptibility of methicillin-resistant S. aureus (MRSA)-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia. MATERIALS AND METHODS: S. aureus was isolated from subclinical mastitis milk specimens. All strains were confirmed by polymerase chain reaction-based detection of staphylococcal nuc, and MRSA was confirmed by the presence of mecA. Antibiotic susceptibility was determined using the disk diffusion method. RESULTS: From 86 milk samples, 49 isolates (57%) were confirmed as S. aureus. All S. aureus isolates were susceptible to tetracycline, gentamicin, chloramphenicol, erythromycin, and trimethoprim/sulfamethoxazole. Nine isolates were identified as MRSA (10.5%). CONCLUSION: In this study, we reported MRSA-associated subclinical mastitis in Bogor, Indonesia. Veterinary World 2021-05 2021-05-13 /pmc/articles/PMC8243663/ /pubmed/34220119 http://dx.doi.org/10.14202/vetworld.2021.1180-1184 Text en Copyright: © Qolbaini, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Qolbaini, Evi Nur
Khoeri, Miftahudin Majid
Salsabila, Korrie
Paramaiswari, Wisiva Tofriska
Tafroji, Wisnu
Artika, I. Made
Safari, Dodi
Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia
title Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia
title_full Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia
title_fullStr Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia
title_full_unstemmed Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia
title_short Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia
title_sort identification and antimicrobial susceptibility of methicillin-resistant staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in bogor, indonesia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243663/
https://www.ncbi.nlm.nih.gov/pubmed/34220119
http://dx.doi.org/10.14202/vetworld.2021.1180-1184
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