Cargando…

Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice

BACKGROUND AND AIM: Brown adipose tissue’s (BAT) ability to increase energy expenditure has become a new focus in obesity research. The amount and activity of BAT are inversely correlated with body-mass index and body fat percentage. Bone morphogenetic protein 7 (BMP7) plays a role in the differenti...

Descripción completa

Detalles Bibliográficos
Autores principales: Syamsunarno, Mas Rizky A. A., Alia, Fenty, Anggraeni, Neni, Sumirat, Vanessa Ayu, Praptama, Suhendra, Atik, Nur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243698/
https://www.ncbi.nlm.nih.gov/pubmed/34220125
http://dx.doi.org/10.14202/vetworld.2021.1234-1240
_version_ 1783715799673864192
author Syamsunarno, Mas Rizky A. A.
Alia, Fenty
Anggraeni, Neni
Sumirat, Vanessa Ayu
Praptama, Suhendra
Atik, Nur
author_facet Syamsunarno, Mas Rizky A. A.
Alia, Fenty
Anggraeni, Neni
Sumirat, Vanessa Ayu
Praptama, Suhendra
Atik, Nur
author_sort Syamsunarno, Mas Rizky A. A.
collection PubMed
description BACKGROUND AND AIM: Brown adipose tissue’s (BAT) ability to increase energy expenditure has become a new focus in obesity research. The amount and activity of BAT are inversely correlated with body-mass index and body fat percentage. Bone morphogenetic protein 7 (BMP7) plays a role in the differentiation and development of BAT, which can be increased by bioactive compounds from several medicinal plants. Moringa oleifera (MO) leaves are rich with vitamin, minerals, and bioactive compounds and have been used for treating obesity-related diseases in the past. The aim of this study was to explore the potency of MO leaf extract (MOLE) to modulate BAT differentiation in mice fed a high-fat diet (HFD). MATERIALS AND METHODS: Twenty-four, 5-week-old male Deutsche Denken Yoken mice (Mus musculus) were randomly divided into four groups: The normal chow diet group was fed a normal diet, the HFD group was fed a HFD, the HFD+MOLE1, and the HFD+MOLE2 groups were fed HFD and MOLE in a dose of 280 and 560 mg/kg body weight (BW)/day, respectively. The experiment was performed for 7 weeks. At the end of the experiment, histological analysis was performed on the interscapular BAT, and blood was drawn for BMP7 protein levels. RESULTS: After 7 weeks, BAT weight in the HFD group was nearly twice in the weight of the HFD+MOLE1 group (125±13.78 mg vs. 75±13.78 mg; p<0.001). There was also a significant increase in BAT cell density in the HFD+MOLE1 group. BMP7 serum protein levels were significantly higher in the HFD+MOLE1 group compared to the HFD group. CONCLUSIONS: The administration of MOLE in a dose of 280 mg/kg BW/day in HFD-mice induces BAT differentiation and proliferation by upregulating BMP7 protein levels.
format Online
Article
Text
id pubmed-8243698
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-82436982021-07-02 Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice Syamsunarno, Mas Rizky A. A. Alia, Fenty Anggraeni, Neni Sumirat, Vanessa Ayu Praptama, Suhendra Atik, Nur Vet World Research Article BACKGROUND AND AIM: Brown adipose tissue’s (BAT) ability to increase energy expenditure has become a new focus in obesity research. The amount and activity of BAT are inversely correlated with body-mass index and body fat percentage. Bone morphogenetic protein 7 (BMP7) plays a role in the differentiation and development of BAT, which can be increased by bioactive compounds from several medicinal plants. Moringa oleifera (MO) leaves are rich with vitamin, minerals, and bioactive compounds and have been used for treating obesity-related diseases in the past. The aim of this study was to explore the potency of MO leaf extract (MOLE) to modulate BAT differentiation in mice fed a high-fat diet (HFD). MATERIALS AND METHODS: Twenty-four, 5-week-old male Deutsche Denken Yoken mice (Mus musculus) were randomly divided into four groups: The normal chow diet group was fed a normal diet, the HFD group was fed a HFD, the HFD+MOLE1, and the HFD+MOLE2 groups were fed HFD and MOLE in a dose of 280 and 560 mg/kg body weight (BW)/day, respectively. The experiment was performed for 7 weeks. At the end of the experiment, histological analysis was performed on the interscapular BAT, and blood was drawn for BMP7 protein levels. RESULTS: After 7 weeks, BAT weight in the HFD group was nearly twice in the weight of the HFD+MOLE1 group (125±13.78 mg vs. 75±13.78 mg; p<0.001). There was also a significant increase in BAT cell density in the HFD+MOLE1 group. BMP7 serum protein levels were significantly higher in the HFD+MOLE1 group compared to the HFD group. CONCLUSIONS: The administration of MOLE in a dose of 280 mg/kg BW/day in HFD-mice induces BAT differentiation and proliferation by upregulating BMP7 protein levels. Veterinary World 2021-05 2021-05-21 /pmc/articles/PMC8243698/ /pubmed/34220125 http://dx.doi.org/10.14202/vetworld.2021.1234-1240 Text en Copyright: © Syamsunarno, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Syamsunarno, Mas Rizky A. A.
Alia, Fenty
Anggraeni, Neni
Sumirat, Vanessa Ayu
Praptama, Suhendra
Atik, Nur
Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice
title Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice
title_full Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice
title_fullStr Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice
title_full_unstemmed Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice
title_short Ethanol extract from Moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice
title_sort ethanol extract from moringa oleifera leaves modulates brown adipose tissue and bone morphogenetic protein 7 in high-fat diet mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243698/
https://www.ncbi.nlm.nih.gov/pubmed/34220125
http://dx.doi.org/10.14202/vetworld.2021.1234-1240
work_keys_str_mv AT syamsunarnomasrizkyaa ethanolextractfrommoringaoleiferaleavesmodulatesbrownadiposetissueandbonemorphogeneticprotein7inhighfatdietmice
AT aliafenty ethanolextractfrommoringaoleiferaleavesmodulatesbrownadiposetissueandbonemorphogeneticprotein7inhighfatdietmice
AT anggraenineni ethanolextractfrommoringaoleiferaleavesmodulatesbrownadiposetissueandbonemorphogeneticprotein7inhighfatdietmice
AT sumiratvanessaayu ethanolextractfrommoringaoleiferaleavesmodulatesbrownadiposetissueandbonemorphogeneticprotein7inhighfatdietmice
AT praptamasuhendra ethanolextractfrommoringaoleiferaleavesmodulatesbrownadiposetissueandbonemorphogeneticprotein7inhighfatdietmice
AT atiknur ethanolextractfrommoringaoleiferaleavesmodulatesbrownadiposetissueandbonemorphogeneticprotein7inhighfatdietmice