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CGRP induces migraine-like symptoms in mice during both the active and inactive phases
BACKGROUND: Circadian patterns of migraine attacks have been reported by patients but remain understudied. In animal models, circadian phases are generally not taken into consideration. In particular, rodents are nocturnal animals, yet they are most often tested during their inactive phase during th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Milan
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243868/ https://www.ncbi.nlm.nih.gov/pubmed/34193048 http://dx.doi.org/10.1186/s10194-021-01277-9 |
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author | Wattiez, Anne-Sophie Gaul, Olivia J. Kuburas, Adisa Zorrilla, Erik Waite, Jayme S. Mason, Bianca N. Castonguay, William C. Wang, Mengya Robertson, Bennett R. Russo, Andrew F. |
author_facet | Wattiez, Anne-Sophie Gaul, Olivia J. Kuburas, Adisa Zorrilla, Erik Waite, Jayme S. Mason, Bianca N. Castonguay, William C. Wang, Mengya Robertson, Bennett R. Russo, Andrew F. |
author_sort | Wattiez, Anne-Sophie |
collection | PubMed |
description | BACKGROUND: Circadian patterns of migraine attacks have been reported by patients but remain understudied. In animal models, circadian phases are generally not taken into consideration. In particular, rodents are nocturnal animals, yet they are most often tested during their inactive phase during the day. This study aims to test the validity of CGRP-induced behavioral changes in mice by comparing responses during the active and inactive phases. METHODS: Male and female mice of the outbred CD1 strain were administered vehicle (PBS) or CGRP (0.1 mg/kg, i.p.) to induce migraine-like symptoms. Animals were tested for activity (homecage movement and voluntary wheel running), light aversive behavior, and spontaneous pain at different times of the day and night. RESULTS: Peripheral administration of CGRP decreased the activity of mice during the first hour after administration, induced light aversive behavior, and spontaneous pain during that same period of time. Both phenotypes were observed no matter what time of the day or night they were assessed. CONCLUSIONS: A decrease in wheel activity is an additional clinically relevant phenotype observed in this model, which is reminiscent of the reduction in normal physical activity observed in migraine patients. The ability of peripheral CGRP to induce migraine-like symptoms in mice is independent of the phase of the circadian cycle. Therefore, preclinical assessment of migraine-like phenotypes can likely be done during the more convenient inactive phase of mice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s10194-021-01277-9. |
format | Online Article Text |
id | pubmed-8243868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Milan |
record_format | MEDLINE/PubMed |
spelling | pubmed-82438682021-06-30 CGRP induces migraine-like symptoms in mice during both the active and inactive phases Wattiez, Anne-Sophie Gaul, Olivia J. Kuburas, Adisa Zorrilla, Erik Waite, Jayme S. Mason, Bianca N. Castonguay, William C. Wang, Mengya Robertson, Bennett R. Russo, Andrew F. J Headache Pain Research Article BACKGROUND: Circadian patterns of migraine attacks have been reported by patients but remain understudied. In animal models, circadian phases are generally not taken into consideration. In particular, rodents are nocturnal animals, yet they are most often tested during their inactive phase during the day. This study aims to test the validity of CGRP-induced behavioral changes in mice by comparing responses during the active and inactive phases. METHODS: Male and female mice of the outbred CD1 strain were administered vehicle (PBS) or CGRP (0.1 mg/kg, i.p.) to induce migraine-like symptoms. Animals were tested for activity (homecage movement and voluntary wheel running), light aversive behavior, and spontaneous pain at different times of the day and night. RESULTS: Peripheral administration of CGRP decreased the activity of mice during the first hour after administration, induced light aversive behavior, and spontaneous pain during that same period of time. Both phenotypes were observed no matter what time of the day or night they were assessed. CONCLUSIONS: A decrease in wheel activity is an additional clinically relevant phenotype observed in this model, which is reminiscent of the reduction in normal physical activity observed in migraine patients. The ability of peripheral CGRP to induce migraine-like symptoms in mice is independent of the phase of the circadian cycle. Therefore, preclinical assessment of migraine-like phenotypes can likely be done during the more convenient inactive phase of mice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s10194-021-01277-9. Springer Milan 2021-06-30 /pmc/articles/PMC8243868/ /pubmed/34193048 http://dx.doi.org/10.1186/s10194-021-01277-9 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Wattiez, Anne-Sophie Gaul, Olivia J. Kuburas, Adisa Zorrilla, Erik Waite, Jayme S. Mason, Bianca N. Castonguay, William C. Wang, Mengya Robertson, Bennett R. Russo, Andrew F. CGRP induces migraine-like symptoms in mice during both the active and inactive phases |
title | CGRP induces migraine-like symptoms in mice during both the active and inactive phases |
title_full | CGRP induces migraine-like symptoms in mice during both the active and inactive phases |
title_fullStr | CGRP induces migraine-like symptoms in mice during both the active and inactive phases |
title_full_unstemmed | CGRP induces migraine-like symptoms in mice during both the active and inactive phases |
title_short | CGRP induces migraine-like symptoms in mice during both the active and inactive phases |
title_sort | cgrp induces migraine-like symptoms in mice during both the active and inactive phases |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243868/ https://www.ncbi.nlm.nih.gov/pubmed/34193048 http://dx.doi.org/10.1186/s10194-021-01277-9 |
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